Archive for June, 2013

Progesterone ELISA kit blog

Test: Progesterone ELISA kit Cat#: 2077Z

Synonyms: Progesterone EIA kit

Shelf Life: 12-14 months

Total time: ~ 110 minutes

 

Progesterone, a neurosteroid is synthesized from both tissue and circulating cholesterol via pregnenolone through sequential enzymatic actions of desmolase and delta 4-5 isomerase.  Progesterone which regulates a wide range of biological and metabolic functions in the body is the precursor to important steroid hormones such as estrogen, testosterone, and cortisol. It is produced primarily in the corpus luteum of the ovaries in females and testes in males, and in the adrenal glands in both sexes.  Body stores progesterone in adipose tissue.  In blood only 2-10% of the total progesterone circulates as free hormone.   Most of the progesterone in blood is in complex form which is bound to Albumin, Corticosteroid Binding Globulin (CBG), and sex Hormone Binding Globulin (SHBG) [1].

Progesterone’s main function in females is to prepare the endometrium in uterus to accept and sustain the fertilized egg.  It promotes fetal survival and development.  The level of progesterone drops if the egg is not fertilized and menstruation follows. Then progesterone level remains relatively constant throughout the follicular phase of the menstrual cycle. Progesterone concentration increases rapidly following ovulation and remains elevated for 4-6 days and decreases to the initial level 24 hours before the onset of menstruation.  During pregnancy placental progesterone production rises steadily to levels of 10 to 20 times those of the luteal phase peak reaching value as high as 200ng/ml (300-400mg daily) at term [2].

 

 

Progesterone Molecular Structure [3]

 

Progesterone measurements are used to determine ovulation as well as to establish luteal phase defects. Thus progesterone level in blood is useful indicator of ovulation and monitoring luteal function.

At Diagnostic Automation the serum Progesterone ELISA kit offers an accurate prediction of ovulation.

http://www.rapidtest.com/index.php?i=Progesterone-ELISA-kit&id=67&cat=14

Fetal adrenal gland uses large amounts of progesterone supplied by the placenta to make cortisol.  Cortisol is an essential hormone for stress response.  Stress reduces progesterone production as body redirects it into cortisol production to manage and decrease stress level in the body [4& 5].

Recent steroid profiling study offers valuable data for the diagnostics of diseases related to steroidogenesis changes in the fetal and maternal compartments and placenta.  Result showed higher production of active progesterone in fetus with the approaching term [6].

 

Moreover, various studies have demonstrated the supportive role of progesterone on normal development of neurons in the brain.   Following traumatic brain injury progesterone is being utilized as a therapeutic mediator to recover neuron loss and improve cognitive dysfunction.  Multiple mechanisms have been proposed for progesterone role as a neuroprotective agent such as reduction of inflammation following brain trauma, and inhibition of calcium signaling [7&8]. A recent study has proposed progesterone as a candidate to be used as a neuroprotective agent for stroke [9].

 

Progesterone concentration can fluctuate considerably not only from person to person, but also its level varies during day, thus serial measurements are highly recommended.

Measuring progesterone level in blood has been an established method for the detection of ovulation.  Progesterone measurements are used clinically to confirm ovulation and normal function of the corpus luteum in non-pregnant women.

At Diagnostic Automation the serum Progesterone ELISA kit offers an accurate prediction of ovulation.

http://www.rapidtest.com/index.php?i=Progesterone-ELISA-kit&id=67&cat=14

 

 

Indications for progesterone elisa testing:

1. Detecting ovulation

2. Monitoring embryo transfer

3. Monitoring fetal health throughout the high risk pregnancies

4. Identifying high risk patients for abortion early on in pregnancy

5. Checking abnormal bleeding in a non-pregnant woman

6. Diagnosis of ectopic & molar pregnancies

7. Monitoring progesterone replacement therapy

8. Checking normal function of luteum in non-pregnant women

9. Diagnosis of Luteinized unruptured follicle syndrome

10. Detecting ovarian tumors

 

 

Intended use of Progesterone ELISA kit Cat#: 2077Z:

The Diagnostic Automation Progesterone EIA test is for the quantitative determination of Progesterone concentration in human serum valid for detection and monitoring of conditions above.

http://www.rapidtest.com/index.php?i=Progesterone-ELISA-kit&id=67&cat=14

 

 

Materials and Components 

Materials provided with Progesterone ELISA Test Kit:

• Goat Anti-Rabbit IgG-coated Microplate 96 wells

• Progesterone ELISA test Reference Standards: 0, 0.5, 3.0, 10, 25, and 50 ng/ml

Liquids, 0.5 ml each, ready to use

• Rabbit Anti-Progesterone Reagent (pink color), 7 ml

• Progesterone elisa-HRP Conjugate Concentrate (11x), 1.3 ml

• Progesterone elisa-HRP Conjugate Diluent, 13 ml

• Progesterone EIA Control 1, Liquid, 0.5 ml, Ready to use

• Progesterone EIA Control 2, Liquid, 0.5 ml, Ready to use

• TMB Reagent (One-Step), 11 ml

• Stop Solution (1N HCl), 11 ml

 

 

Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled or deionized water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader

 

In addition to progesterone ELISA kit, Diagnostic Automation offers two additional tests for assessment of progesterone level in blood:

17-alpha hydroxy Progesterone ELISA kit (Cat # 1292Z)

Diagnostic Automation 17-Hydroxyprogesterone (17-OH progesterone or 17OHP) elisa kit is expedient for the measurements of levels of 17-OHP which are useful in the evaluation of patients suspected with congenital adrenal hyperplasia.  Since defective enzymes, 21-hydroxylase and 11ß-hydroxilase lead to a buildup of 17OHP level.

http://www.rapidtest.com/index.php?i=17-alpha-hydroxy-Progesterone-ELISA-kit&id=72&cat=14

Progesterone CLIA (Chemiluminescence Immuno Assay) kit (Cat # 9025-16)

Progesterone CLIA detection using Microplate luminometers provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.

http://www.rapidtest.com/index.php?i=Progesterone-CLIA-kit—(Chemiluminescence-Immuno-Assay)&id=535&cat=39

 

 

 

Related kits to progesterone elisa:

 

Beta HCG (Total) ELISA kit (Cat # 4201-16)

Studies have shown that HCG levels can be useful in predicting spontaneous abortions, aiding in the detection of ectopic pregnancy and multiple gestation. Elevated levels of HCG have also been detected in serum from patients with abnormal physiological conditions not related to pregnancy.

http://www.rapidtest.com/index.php?i=Beta-HCG-(Total)-ELISA-kit&id=58&cat=13

 

Luteinizing hormone (LH) ELISA Kit (Cat #4225Z)

This test may be used in conjunction with progesterone elisa kit.  Patients suffering from hypogonadism show increased concentrations of serum LH.  A decrease in steroid hormone production in females is a result of immature ovaries, primary ovarian failure, polycystic ovary disease, or menopause; in these cases, LH secretion is not regulated.

http://www.rapidtest.com/index.php?i=LH-ELISA-kit&id=61&cat=13

 

 

 

 

 

References:

1.Jeremy M Berg, John L Tymoczko, and Lubert Stryer. Biochemistry, 5th edition.2002, New York.  WH. Freeman; ISBN-10: 0-7167-3051-0

2. Hoehn K, Marieb EN. Human Anatomy & Physiology, 2010, San Francisco: Benjamin Cummings; ISBN 0-321-60261-7

3. NCBI

http://pubchem.ncbi.nlm.nih.gov/image/structurefly.cgi?cid=6238

5. Mastorako G, Ilas I. Maternal and fetal hypothalamic-pituitary-adrenal axes during pregnancy and postpartum. Ann N Y Acad Sci. 2003, 997:136-49

6. Hill M, et al. Steroid profiling in pregnancy: A focus on the human fetus, 2013 Apr 10.  J. Steroid Biochem Mol Biol.

7. Pan DS, Liu WG, Yang XF, Cao F (October 2007). Inhibitory effect of progesterone on inflammatory factors after experimental traumatic brain injury. 2007, Biomed. Environ. Sci. 20 (5): 432–8

8. Luoma JI, Stern CM, Mermelstein PG (August 2012). Progesterone inhibition of neuronal calcium signaling underlies aspects of progesterone-mediated neuroprotection. 2012,  J. Steroid Biochem. Mol. Biol. 131 (1-2): 30–6

9. Wong R, Bath PM et al. Progesterone and cerebral ischaemia: the relevance of ageing, 2013 Apr 30. J Neuroendocrinol.