Archive for the ‘Ferritin ELISA kits’ Category

Ferritin ELISA Kit blog

Test: Ferritin ELISA Kit

Synonyms: Ferritin EIA

Shelf Life: 12-14 months

Total time: ~ 80 minutes

 

Ferritin elisa kit:

Ferritin is an iron storage protein and a key regulator of iron level in the body with each molecule capable of binding up to 4000 iron atoms. Its main function is storing a soluble and nontoxic form of iron.  Ferritin can store iron and release it in a controlled manner to maintain a balanced level of iron in the body.  Thus ferritin is essential for controlling both iron deficiency and iron overload in the body.

Human gene encoding the major iron storage protein ferritin is FTH1 (heavy polypeptide1).  Defect in ferritin proteins are linked to neurodegenerative diseases [1].

The ideal level of ferritin is 40-60 nanogram/milliliter (ng/ml), below 20ng/ml is deficiency and above 80ng/ml is iron overload.

Ferritin is found in the skeletal muscle (meat), liver, spleen and bone marrow.  A small amount of ferritin is in the blood and that amount of ferritin depicts the iron storage in the body.

Diagnostic Automation offers a rapid and reliable tool as an ELISA kit for measuring the serum concentration of ferritin in blood.

 

 

 

 

Molecular Structure of Human Ferritin [2]

What is Iron deficiency?

Iron deficiency with a complex mechanism is one of the most common nutritional disorders, and at its most extreme it can develop into iron deficiency anemia. Anemia occurs when there is a reduction in the number of red blood cells or red blood cells have a very low level of hemoglobin.  Anemia is common in children and menstruating women typically between ages 14 to 52.  Iron has many functions in human body and one of the most important functions is binding to hemoglobin molecule to serve as an oxygen carrier in every cell of the body [3].

Iron plays fundamental roles in human health, thus maintaining a sufficient supply of iron is crucial.  There are multiple processes through which human body loses iron such as bleeding (especially in menstrual periods in women), sweating, and urination leading to iron deficiency.

Normal function of hemoglobin and multiple enzymes in our body depend on iron.  The absorbance of iron is selective, however our body constantly loses small amount of iron.

It is important to diagnose the underlying cause of anemia since there are multiple factors contributing to anemia such as:

Nutritional deficiency (e.g. vitamin B12 and/or folate)

Genetic anemia such as sickle cell, thalassemia

Cancer

Acute blood loss

Although diagnosis of anemia is easy, but identifying the correct cause of it is crucial as in some anemia patients such as in thalassemia iron overload is the major problem not the deficiency.

Iron is a potent oxidative stressor which can oxidize susceptible cells or component of a cell.  The oxidizing process generates free radicals which can cause substantial damage to the cells and increase the risk of heart diseases by damaging the inner lining cells of the blood vessels or can cause cancer by damaging the DNA of the cells.

At Diagnostic Automation, we offer the Ferritin elisa kit (Ferritin elisa test), a fast (total time ~ 80 minutes) and reliable test to measure the ferritin level in the serum.

In contrast to iron deficiency, there are also iron overload conditions which can cause serious damage to multiple organs.  This increase in iron generally appears in patients who have regular blood transfusions, or have a genetic predisposition to iron overload disorders such as Hemochromatosis (absorbance of too much of dietary iron), Hemolytic anemia, and Hemosiderosis. Hemochromatosis treatment consists of removing blood from the patient to reduce the amount of iron [4].  Monitoring and guided treatment for iron overloads disorders such as hemochromatosis and hemosiderosis are easily done using Diagnostic Automation Ferritin elisa kit.  In addition to these clinical conditions, processed foods fortified with iron are another source of iron overload.  Moreover, high level of ferritin in blood can be indicative of inflammatory disease, liver problem, rheumatoid arthritis, hyperthyroidism, or cancer.

Finally, iron builds strong red blood cells, and ferritin is one of the body’s primary iron-storage proteins.  Thus, ferritin screening is one of the most important proactive and preventive health tests.  At Diagnostic Automation we offer ferritin elisa test (ferritin EIA) as a serum ferritin elisa kit for a rapid, sensitive, and reliable assessment of ferritin level in blood for diagnosis of both iron deficiency and iron overload.

 

 

Ferritin ELISA kit

 

 

Indications for ferritin testing:

 

Ferritin elisa test is performed for two purposes, diagnosing a disorder or monitoring a clinical condition:

 

A.    Diagnosing a disorder

1. Low hemoglobin level

2. Low hematocrit

3. Hemochromatosis

4. Liver diseases

5. Cancers

 

In combination with a total iron binding capacity and iron test a low ferritin test result using ferritin elisa kit can confirm the iron deficiency anemia.

 

B.     Monitoring iron status

  1. Hemochromatosis and hemosiderosis
  2. Pregnant women
  3. Blood donors
  4. Blood transfusion
  5. Renal dialysis patients
  6. Inflammation
  7. Diabetes type II
  8. Hyperthyroidism
  9. Leukemia
  10. Rheumatoid arthritis

 

Intended use of Ferritin ELISA kit Cat # 1601Z:

The Diagnostic Automation Ferritin EIA test is for the quantitative determination of Ferritin concentration in human serum valid for either diagnosing a disorder or monitoring iron status of conditions mentioned above.

 

http://www.rapidtest.com/index.php?i=Ferritin-ELISA-kit&id=40&cat=159

 

 

 

 

Materials and Components

 

Materials provided with Ferritin ELISA Test Kit:

• Antibody-coated ELISA Microplate 96 wells

• Ferritin EIA test reference standard set, contains 0, 10, 50, 100, 400, &

800 ng/ml, (NIBSC-WHO 80/602, human liver standard), ready for use

• Ferritin EIA enzyme conjugate reagent, 12 ml

• TMB Substrate, 12 ml

• Stop Solution, 12 ml

• Ferritin ELISA test wash Buffer Concentrate (50X), 15ml

Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader

 

In addition to Ferritin ELISA kit, Diagnostic Automation offers two additional tests for assessment of iron level:

1. Ferritin CLIA kit (Cat # 9019-16); a Chemiluminescence Immuno Assay detection using Microplate luminometers. The Ferritin CLIA test utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact ferritin molecule.  Ferritin CLIA kit provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.

http://www.rapidtest.com/index.php?i=Ferritin-CLIA-kits—(Chemiluminescence-Immuno-Assay)&id=570&cat=47

 

2. Soluble Transferrin Receptor (sTfR) ELISA kit (Cat # 3126-15) has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia (IDA) and anemia of chronic disease (ACD).  Distinguishing between IDA and ACD is a key step for determining whether iron supplementation would be beneficial.  sTfR ELISA test  is similarly useful for monitoring erythropoiesis in malignancy and chronic renal disease.

 

http://www.rapidtest.com/index.php?i=sTfR-Soluble-Transferrin-Soluble-Receptor-&id=710&cat=159

 

 

 

References:

1. Molecular structure of ferritin:  NCBI

http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=65592

2. Ferritin gene: http://www.ncbi.nlm.nih.gov/gene/2495

3. Polin V et al. Iron deficiency: From diagnosis to treatment. Dig Liver Dis. 2013

4. Crownover BK, Covey CJ. Hereditary hemochromatosis. Am Fam Physician. 2013, 1;87(3):183-90

5. Muñoz M, García-Erce JA, Remacha ÁF. Disorders of iron metabolism. Part II: iron deficiency and iron overload. J Clin Pathol. 2011 Apr;64(4):287-96.

FERRITIN ELISA KITS | IRON DEFICIENCY | ANEMIA

FERRITIN ELISA KITS

 

WHAT IS IRON DEFICIENCY?

 

Iron deficiency is one of the most common nutritional disorders today, and at its most extreme, this iron deficiency can develop into iron deficiency anemia.  Anemia usually occurs when a lower than normal number of red blood cells is present, or if the red blood cells do not contain enough hemoglobin.

Iron builds strong red blood cells, and Ferritin is one of the body’s primary iron-storage proteins.  Thus, measuring ferritin in serum is useful in understanding iron deficiency, or in some cases, an abundance of iron.  This increase in iron generally appears in patients who have regular blood transfusions, or have a predisposed genetic disorder.

 

ADVANTAGES OF FERRITIN ELISA KITS

 

Ferritin ELISA kit

 

 

Ferritin ELISA Kits are perfect testing methods for determining the amount of Ferritin concentration in human serum.   Ferritin ELISA Kits are rapid, sensitive, and reliable.  The antibodies developed for the Ferritin ELISA Kits can determine a minimum concentration of human ferritin of 5 ng/ml.  The Ferritin ELISA Kits are also important for laboratory monitoring of the iron levels of pregnant women, blood donors, and renal dialysis patients.

Ferritin ELISA Kits are based on a solid phase enzyme-linked immunosorbent assay. Ferritin ELISA kits utilize one anti-ferritin antibody for solid phase immobilization, and another mouse monoclonal anti-ferritin antibody in the antibody-enzyme conjugate solution.  The Ferritin ELISA Kit sample is allowed to react simultaneously with the antibodies, resulting in the ferritin molecules being sandwiched between the solid phase and enzyme-linked antibodies.

 

Ferritin ELISA Kits have a Sensitivity of 5.0ng/ml and a Specificity of 98.7%.  Total time to obtain results of Ferritin ELISA kits, is approximately 80 minutes.

 

Like other ELISA Kits, Ferritin ELISA Kits have many notable features:

  • User-friendly directions and explanation of test procedures
  • Simple and safe reagent preparation
  • Clear instructions on specimen collection
  • Comprehensive package of required materials
  • Explicit quality control and storage guidelines
  • Reliable and easy-to-read test results

For more information on Ferritin ELISA Kits or any other ELISA Kits, please refer to our main website page [click here].

 

Los-Angeles based Diagnostic Automation/Cortez Diagnostics, Inc. prides itself on offering simple, convenient, and inexpensive ELISA Kits worldwide.   For further information on these ELISA Kits, or any other diagnostic medical testing equipment, including Rapid Tests, IFA Kits, CLIA test kits, Serology, and Instrumentation, please contact our Customer Service Representatives at 818-591-3030.