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Progesterone ELISA kit blog

Test: Progesterone ELISA kit Cat#: 2077Z

Synonyms: Progesterone EIA kit

Shelf Life: 12-14 months

Total time: ~ 110 minutes


Progesterone, a neurosteroid is synthesized from both tissue and circulating cholesterol via pregnenolone through sequential enzymatic actions of desmolase and delta 4-5 isomerase.  Progesterone which regulates a wide range of biological and metabolic functions in the body is the precursor to important steroid hormones such as estrogen, testosterone, and cortisol. It is produced primarily in the corpus luteum of the ovaries in females and testes in males, and in the adrenal glands in both sexes.  Body stores progesterone in adipose tissue.  In blood only 2-10% of the total progesterone circulates as free hormone.   Most of the progesterone in blood is in complex form which is bound to Albumin, Corticosteroid Binding Globulin (CBG), and sex Hormone Binding Globulin (SHBG) [1].

Progesterone’s main function in females is to prepare the endometrium in uterus to accept and sustain the fertilized egg.  It promotes fetal survival and development.  The level of progesterone drops if the egg is not fertilized and menstruation follows. Then progesterone level remains relatively constant throughout the follicular phase of the menstrual cycle. Progesterone concentration increases rapidly following ovulation and remains elevated for 4-6 days and decreases to the initial level 24 hours before the onset of menstruation.  During pregnancy placental progesterone production rises steadily to levels of 10 to 20 times those of the luteal phase peak reaching value as high as 200ng/ml (300-400mg daily) at term [2].



Progesterone Molecular Structure [3]


Progesterone measurements are used to determine ovulation as well as to establish luteal phase defects. Thus progesterone level in blood is useful indicator of ovulation and monitoring luteal function.

At Diagnostic Automation the serum Progesterone ELISA kit offers an accurate prediction of ovulation.

Fetal adrenal gland uses large amounts of progesterone supplied by the placenta to make cortisol.  Cortisol is an essential hormone for stress response.  Stress reduces progesterone production as body redirects it into cortisol production to manage and decrease stress level in the body [4& 5].

Recent steroid profiling study offers valuable data for the diagnostics of diseases related to steroidogenesis changes in the fetal and maternal compartments and placenta.  Result showed higher production of active progesterone in fetus with the approaching term [6].


Moreover, various studies have demonstrated the supportive role of progesterone on normal development of neurons in the brain.   Following traumatic brain injury progesterone is being utilized as a therapeutic mediator to recover neuron loss and improve cognitive dysfunction.  Multiple mechanisms have been proposed for progesterone role as a neuroprotective agent such as reduction of inflammation following brain trauma, and inhibition of calcium signaling [7&8]. A recent study has proposed progesterone as a candidate to be used as a neuroprotective agent for stroke [9].


Progesterone concentration can fluctuate considerably not only from person to person, but also its level varies during day, thus serial measurements are highly recommended.

Measuring progesterone level in blood has been an established method for the detection of ovulation.  Progesterone measurements are used clinically to confirm ovulation and normal function of the corpus luteum in non-pregnant women.

At Diagnostic Automation the serum Progesterone ELISA kit offers an accurate prediction of ovulation.



Indications for progesterone elisa testing:

1. Detecting ovulation

2. Monitoring embryo transfer

3. Monitoring fetal health throughout the high risk pregnancies

4. Identifying high risk patients for abortion early on in pregnancy

5. Checking abnormal bleeding in a non-pregnant woman

6. Diagnosis of ectopic & molar pregnancies

7. Monitoring progesterone replacement therapy

8. Checking normal function of luteum in non-pregnant women

9. Diagnosis of Luteinized unruptured follicle syndrome

10. Detecting ovarian tumors



Intended use of Progesterone ELISA kit Cat#: 2077Z:

The Diagnostic Automation Progesterone EIA test is for the quantitative determination of Progesterone concentration in human serum valid for detection and monitoring of conditions above.



Materials and Components 

Materials provided with Progesterone ELISA Test Kit:

• Goat Anti-Rabbit IgG-coated Microplate 96 wells

• Progesterone ELISA test Reference Standards: 0, 0.5, 3.0, 10, 25, and 50 ng/ml

Liquids, 0.5 ml each, ready to use

• Rabbit Anti-Progesterone Reagent (pink color), 7 ml

• Progesterone elisa-HRP Conjugate Concentrate (11x), 1.3 ml

• Progesterone elisa-HRP Conjugate Diluent, 13 ml

• Progesterone EIA Control 1, Liquid, 0.5 ml, Ready to use

• Progesterone EIA Control 2, Liquid, 0.5 ml, Ready to use

• TMB Reagent (One-Step), 11 ml

• Stop Solution (1N HCl), 11 ml



Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled or deionized water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader


In addition to progesterone ELISA kit, Diagnostic Automation offers two additional tests for assessment of progesterone level in blood:

17-alpha hydroxy Progesterone ELISA kit (Cat # 1292Z)

Diagnostic Automation 17-Hydroxyprogesterone (17-OH progesterone or 17OHP) elisa kit is expedient for the measurements of levels of 17-OHP which are useful in the evaluation of patients suspected with congenital adrenal hyperplasia.  Since defective enzymes, 21-hydroxylase and 11ß-hydroxilase lead to a buildup of 17OHP level.

Progesterone CLIA (Chemiluminescence Immuno Assay) kit (Cat # 9025-16)

Progesterone CLIA detection using Microplate luminometers provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.—(Chemiluminescence-Immuno-Assay)&id=535&cat=39




Related kits to progesterone elisa:


Beta HCG (Total) ELISA kit (Cat # 4201-16)

Studies have shown that HCG levels can be useful in predicting spontaneous abortions, aiding in the detection of ectopic pregnancy and multiple gestation. Elevated levels of HCG have also been detected in serum from patients with abnormal physiological conditions not related to pregnancy.


Luteinizing hormone (LH) ELISA Kit (Cat #4225Z)

This test may be used in conjunction with progesterone elisa kit.  Patients suffering from hypogonadism show increased concentrations of serum LH.  A decrease in steroid hormone production in females is a result of immature ovaries, primary ovarian failure, polycystic ovary disease, or menopause; in these cases, LH secretion is not regulated.







1.Jeremy M Berg, John L Tymoczko, and Lubert Stryer. Biochemistry, 5th edition.2002, New York.  WH. Freeman; ISBN-10: 0-7167-3051-0

2. Hoehn K, Marieb EN. Human Anatomy & Physiology, 2010, San Francisco: Benjamin Cummings; ISBN 0-321-60261-7


5. Mastorako G, Ilas I. Maternal and fetal hypothalamic-pituitary-adrenal axes during pregnancy and postpartum. Ann N Y Acad Sci. 2003, 997:136-49

6. Hill M, et al. Steroid profiling in pregnancy: A focus on the human fetus, 2013 Apr 10.  J. Steroid Biochem Mol Biol.

7. Pan DS, Liu WG, Yang XF, Cao F (October 2007). Inhibitory effect of progesterone on inflammatory factors after experimental traumatic brain injury. 2007, Biomed. Environ. Sci. 20 (5): 432–8

8. Luoma JI, Stern CM, Mermelstein PG (August 2012). Progesterone inhibition of neuronal calcium signaling underlies aspects of progesterone-mediated neuroprotection. 2012,  J. Steroid Biochem. Mol. Biol. 131 (1-2): 30–6

9. Wong R, Bath PM et al. Progesterone and cerebral ischaemia: the relevance of ageing, 2013 Apr 30. J Neuroendocrinol.





Ferritin ELISA Kit blog

Test: Ferritin ELISA Kit

Synonyms: Ferritin EIA

Shelf Life: 12-14 months

Total time: ~ 80 minutes


Ferritin elisa kit:

Ferritin is an iron storage protein and a key regulator of iron level in the body with each molecule capable of binding up to 4000 iron atoms. Its main function is storing a soluble and nontoxic form of iron.  Ferritin can store iron and release it in a controlled manner to maintain a balanced level of iron in the body.  Thus ferritin is essential for controlling both iron deficiency and iron overload in the body.

Human gene encoding the major iron storage protein ferritin is FTH1 (heavy polypeptide1).  Defect in ferritin proteins are linked to neurodegenerative diseases [1].

The ideal level of ferritin is 40-60 nanogram/milliliter (ng/ml), below 20ng/ml is deficiency and above 80ng/ml is iron overload.

Ferritin is found in the skeletal muscle (meat), liver, spleen and bone marrow.  A small amount of ferritin is in the blood and that amount of ferritin depicts the iron storage in the body.

Diagnostic Automation offers a rapid and reliable tool as an ELISA kit for measuring the serum concentration of ferritin in blood.





Molecular Structure of Human Ferritin [2]

What is Iron deficiency?

Iron deficiency with a complex mechanism is one of the most common nutritional disorders, and at its most extreme it can develop into iron deficiency anemia. Anemia occurs when there is a reduction in the number of red blood cells or red blood cells have a very low level of hemoglobin.  Anemia is common in children and menstruating women typically between ages 14 to 52.  Iron has many functions in human body and one of the most important functions is binding to hemoglobin molecule to serve as an oxygen carrier in every cell of the body [3].

Iron plays fundamental roles in human health, thus maintaining a sufficient supply of iron is crucial.  There are multiple processes through which human body loses iron such as bleeding (especially in menstrual periods in women), sweating, and urination leading to iron deficiency.

Normal function of hemoglobin and multiple enzymes in our body depend on iron.  The absorbance of iron is selective, however our body constantly loses small amount of iron.

It is important to diagnose the underlying cause of anemia since there are multiple factors contributing to anemia such as:

Nutritional deficiency (e.g. vitamin B12 and/or folate)

Genetic anemia such as sickle cell, thalassemia


Acute blood loss

Although diagnosis of anemia is easy, but identifying the correct cause of it is crucial as in some anemia patients such as in thalassemia iron overload is the major problem not the deficiency.

Iron is a potent oxidative stressor which can oxidize susceptible cells or component of a cell.  The oxidizing process generates free radicals which can cause substantial damage to the cells and increase the risk of heart diseases by damaging the inner lining cells of the blood vessels or can cause cancer by damaging the DNA of the cells.

At Diagnostic Automation, we offer the Ferritin elisa kit (Ferritin elisa test), a fast (total time ~ 80 minutes) and reliable test to measure the ferritin level in the serum.

In contrast to iron deficiency, there are also iron overload conditions which can cause serious damage to multiple organs.  This increase in iron generally appears in patients who have regular blood transfusions, or have a genetic predisposition to iron overload disorders such as Hemochromatosis (absorbance of too much of dietary iron), Hemolytic anemia, and Hemosiderosis. Hemochromatosis treatment consists of removing blood from the patient to reduce the amount of iron [4].  Monitoring and guided treatment for iron overloads disorders such as hemochromatosis and hemosiderosis are easily done using Diagnostic Automation Ferritin elisa kit.  In addition to these clinical conditions, processed foods fortified with iron are another source of iron overload.  Moreover, high level of ferritin in blood can be indicative of inflammatory disease, liver problem, rheumatoid arthritis, hyperthyroidism, or cancer.

Finally, iron builds strong red blood cells, and ferritin is one of the body’s primary iron-storage proteins.  Thus, ferritin screening is one of the most important proactive and preventive health tests.  At Diagnostic Automation we offer ferritin elisa test (ferritin EIA) as a serum ferritin elisa kit for a rapid, sensitive, and reliable assessment of ferritin level in blood for diagnosis of both iron deficiency and iron overload.



Ferritin ELISA kit



Indications for ferritin testing:


Ferritin elisa test is performed for two purposes, diagnosing a disorder or monitoring a clinical condition:


A.    Diagnosing a disorder

1. Low hemoglobin level

2. Low hematocrit

3. Hemochromatosis

4. Liver diseases

5. Cancers


In combination with a total iron binding capacity and iron test a low ferritin test result using ferritin elisa kit can confirm the iron deficiency anemia.


B.     Monitoring iron status

  1. Hemochromatosis and hemosiderosis
  2. Pregnant women
  3. Blood donors
  4. Blood transfusion
  5. Renal dialysis patients
  6. Inflammation
  7. Diabetes type II
  8. Hyperthyroidism
  9. Leukemia
  10. Rheumatoid arthritis


Intended use of Ferritin ELISA kit Cat # 1601Z:

The Diagnostic Automation Ferritin EIA test is for the quantitative determination of Ferritin concentration in human serum valid for either diagnosing a disorder or monitoring iron status of conditions mentioned above.





Materials and Components


Materials provided with Ferritin ELISA Test Kit:

• Antibody-coated ELISA Microplate 96 wells

• Ferritin EIA test reference standard set, contains 0, 10, 50, 100, 400, &

800 ng/ml, (NIBSC-WHO 80/602, human liver standard), ready for use

• Ferritin EIA enzyme conjugate reagent, 12 ml

• TMB Substrate, 12 ml

• Stop Solution, 12 ml

• Ferritin ELISA test wash Buffer Concentrate (50X), 15ml

Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader


In addition to Ferritin ELISA kit, Diagnostic Automation offers two additional tests for assessment of iron level:

1. Ferritin CLIA kit (Cat # 9019-16); a Chemiluminescence Immuno Assay detection using Microplate luminometers. The Ferritin CLIA test utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact ferritin molecule.  Ferritin CLIA kit provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.—(Chemiluminescence-Immuno-Assay)&id=570&cat=47


2. Soluble Transferrin Receptor (sTfR) ELISA kit (Cat # 3126-15) has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia (IDA) and anemia of chronic disease (ACD).  Distinguishing between IDA and ACD is a key step for determining whether iron supplementation would be beneficial.  sTfR ELISA test  is similarly useful for monitoring erythropoiesis in malignancy and chronic renal disease.





1. Molecular structure of ferritin:  NCBI

2. Ferritin gene:

3. Polin V et al. Iron deficiency: From diagnosis to treatment. Dig Liver Dis. 2013

4. Crownover BK, Covey CJ. Hereditary hemochromatosis. Am Fam Physician. 2013, 1;87(3):183-90

5. Muñoz M, García-Erce JA, Remacha ÁF. Disorders of iron metabolism. Part II: iron deficiency and iron overload. J Clin Pathol. 2011 Apr;64(4):287-96.






Iron deficiency is one of the most common nutritional disorders today, and at its most extreme, this iron deficiency can develop into iron deficiency anemia.  Anemia usually occurs when a lower than normal number of red blood cells is present, or if the red blood cells do not contain enough hemoglobin.

Iron builds strong red blood cells, and Ferritin is one of the body’s primary iron-storage proteins.  Thus, measuring ferritin in serum is useful in understanding iron deficiency, or in some cases, an abundance of iron.  This increase in iron generally appears in patients who have regular blood transfusions, or have a predisposed genetic disorder.




Ferritin ELISA kit



Ferritin ELISA Kits are perfect testing methods for determining the amount of Ferritin concentration in human serum.   Ferritin ELISA Kits are rapid, sensitive, and reliable.  The antibodies developed for the Ferritin ELISA Kits can determine a minimum concentration of human ferritin of 5 ng/ml.  The Ferritin ELISA Kits are also important for laboratory monitoring of the iron levels of pregnant women, blood donors, and renal dialysis patients.

Ferritin ELISA Kits are based on a solid phase enzyme-linked immunosorbent assay. Ferritin ELISA kits utilize one anti-ferritin antibody for solid phase immobilization, and another mouse monoclonal anti-ferritin antibody in the antibody-enzyme conjugate solution.  The Ferritin ELISA Kit sample is allowed to react simultaneously with the antibodies, resulting in the ferritin molecules being sandwiched between the solid phase and enzyme-linked antibodies.


Ferritin ELISA Kits have a Sensitivity of 5.0ng/ml and a Specificity of 98.7%.  Total time to obtain results of Ferritin ELISA kits, is approximately 80 minutes.


Like other ELISA Kits, Ferritin ELISA Kits have many notable features:

  • User-friendly directions and explanation of test procedures
  • Simple and safe reagent preparation
  • Clear instructions on specimen collection
  • Comprehensive package of required materials
  • Explicit quality control and storage guidelines
  • Reliable and easy-to-read test results

For more information on Ferritin ELISA Kits or any other ELISA Kits, please refer to our main website page [click here].


Los-Angeles based Diagnostic Automation/Cortez Diagnostics, Inc. prides itself on offering simple, convenient, and inexpensive ELISA Kits worldwide.   For further information on these ELISA Kits, or any other diagnostic medical testing equipment, including Rapid Tests, IFA Kits, CLIA test kits, Serology, and Instrumentation, please contact our Customer Service Representatives at 818-591-3030.