Archive for the ‘Dengue elisa kit’ Category
Dengue ELISA test | Rapidtest.com Blog
Have you gotten sick after traveling abroad to areas like Latin America, Southeast Asia, or the Pacific Islands? If you were stung by a mosquito, there is a possibility that you could have been infected with dengue virus. Dengue infection can be complicated but early recognition and treatments can lower the risks, such as medical complications or death. Diagnostic Automation / Cortez Diagnostics, Inc., offers quality dengue ELISA tests for your needs.
Typical dengue symptoms are usually high fever, joint pain, bone pain, and rashes. It’s important to watch for any warning signs and go to the emergency room if you are having severe stomach pains, vomiting, red spots, or difficulty breathing. Typically, there have been very few cases in the US, but there was an outbreak in 2001 in Hawaii. When traveling, it’s important to use bug spray and other pest control methods to avoid being bitten. No one wants to travel with this troublesome disease!
Take a look at our dengue ELISA tests and make your purchase today. Whether you are an individual who suspects a problem or are working for a testing lab and need to run this test, Diagnostic Automation / Cortez Diagnostics, Inc., offers excellent tests for your needs.
Like other specific Dengue ELISA kits, the Dengue IgM ELISA Kits are enzyme-linked immunosorbent assays for the qualitative identification of antibodies to dengue in human serum.
Serological testing is a common method of identifying dengue antibodies, and Dengue ELISA Kits have been found to be some of the most useful methods for bringing about exact serological diagnosis of dengue infection.
Because of their sensitivity, capability of handling many samples (96 wells), and potential for automation, Dengue IgM ELISA kits have been taking the place of other procedures that detect IgM antibodies. Dengue IgM ELISA Kits are quick, easy-to-use, effective in diagnosing patients, and are reliable. Dengue IgM ELISA kits are highly sensitive assays that detect dengue antibodies. Sensitivity of Dengue IgM ELISA kits is 86% and Specificity is 100%. In addition, results of Dengue IgM ELISA Kits can be obtained is less than 30 minutes.
PRINCIPLE OF DENGUE IgM ELISA KITS
The principle of Dengue IgM ELISA kits involves three incubation steps (10 minutes each). Purified dengue virus antigen from Vero cell cultured type 1-4 dengue is coated on the microwells before adding patient’s sera at the first incubation procedure. During the second incubation step, the added enzyme conjugate will bind to any antibodies present. The third incubation involves the addition of TMB chromogen. ELISA kit readers can be used to obtain results, or the results can be read visually.
NOTABLE FEATURES OF DENGUE IgM ELISA KITS:
- User-friendly directions and explanation of test procedures
- Simple and safe reagent preparation
- Clear instructions on specimen collection
- Comprehensive package of required materials
- Explicit quality control and storage guidelines
- Reliable and easy-to-read test results
See the Dengue IgM Product Page/Insert for more information. Click here.
Other reliable ELISA Kits in the diagnosis of dengue infection are Dengue IgG ELISA Kits, Dengue IgG/IgM ELISA kits, and Dengue NS1 ELISA kits for detection of NS1 antigens in human serum. For more information on any of the Dengue ELISA kits or any other ELISA Kits, Parasitology ELISA kits, Rapid Test, IFA Kit, CLIA Test, or Serology Test, please check our website [ here] or contact our Customer Service Department at 818-591-3030.
Los Angeles-based Diagnostic Automation/Cortez Diagnostics, Inc., a global leader in the field of manufacturing and distributing medical testing equipment, is pleased to offer a top quality Dengue NS1 Rapid Test kit at an affordable price. This inexpensive Dengue NS1 Rapid Test is known for its superior sensitivity and specificity in accurately detecting early Dengue infections. This Dengue NS1 Rapid Test is a qualitative membrane-based immunoassay for the detection of NS1 antigen in human serum. Notable features of this Dengue NS1 Rapid Test are its safe, convenient, and easy-to-use test procedures and accurate, quick results.
DENGUE NS1 RAPID TEST KIT – TIMELY AND SENSITIVE
Los Angeles based Diagnostic Automation/Cortez Diagnostics, Inc. has been a leader in the development of infectious disease rapid tests for more than 20 years. In the past, some available tools for dengue virus detection have been time-consuming. However, this particular Dengue NS1 Rapid Test now being offered, is proven to be timely, sensitive, and specific when dealing with early detection of the Dengue NS1 virus. The use of tests like the Dengue NS1 Rapid Test as a first-line test for the screening of dengue-infected patients, could help accelerate the diagnosis of dengue fever in clinical diagnostic laboratories. Rapid and easy diagnosis of dengue using Dengue NS1 Rapid Test, can assist patient triage and care management. For example, during an epidemic outbreak, the ability to triage those patients suspected of having dengue fever and to quickly establish an anti-viral therapy with use of a Dengue NS1 Rapid Test, might improve the rates of disease control drastically. Thus, the Dengue NS1 Rapid Test presents the ideal method of testing as it is rapid, easily applicable, sensitive, and highly specific. Available to customers worldwide, the Dengue NS1 Rapid Test Kit is offered in a strip format only.
This One-Step Dengue NS1 RapiDip Insta Test (strip format) is a qualitative, membrane based immunoassay for the detection of non-structural protein 1 (NS1) antigen in human serum. The Dengue NS1 Rapid Test membrane is pre-coated with a NS1 specific antibody on the Dengue NS1 Rapid Test line region and utilizes a separate control to assure assay flow and performance. If NS1 antigen is present, a red line will appear on the Dengue NS1 Rapid Test line. The entire procedure using Dengue NS1 Rapid Test-strip is approximately 30 minutes. Each Dengue NS1 Rapid Test Kit contains 25 Dengue NS1 Rapid Test dipsticks, sixty-four (64) individual sample reservoir wells, and one vial of Chase Buffer, Type A. Multiple Dengue NS1 Rapid Test Kits (strip format) can be shipped in packages of 25 or 50.
RELATED DENGUE RAPID TEST KIT – NOTABLE FEATURES
Closely related to the Dengue NS1 Rapid Test Kit is another product in the field of Dengue IgG/IgM Antibody virus detection — the Dengue Rapid Test Kit (cassette format.) Specifically labeled as the One-Step Dengue RapiCard Insta Test, this test is an immunochromatographic assay for the simultaneous detection of IgG and IgM antibodies to Dengue virus in human whole blood, serum, or plasma. This Dengue Rapid Test-cassette is used as a screening test for Dengue viral infection and as an aid for differential diagnosis of primary and secondary infections in conjunction with other criteria. This Dengue Rapid Test-cassette is a new generation immunochromatographic test using recombinant dengue viral antigens of all four serotypes to detect specific antibody response. The Dengue Rapid Test Kit – cassette utilizes the principle of immunochromatography where mouse anti-human IgM and human IgG antibodies are immobilized as two individual test lines. As the Dengue Rapid Test sample flows through the membrane assembly, a colored band appears which confirms a positive test result. Absence of the colored band in the Dengue Rapid Test window indicates a negative test result. A notable feature of this Dengue Rapid Test-cassette is that a built-in control line will always appear in the Dengue Rapid Test window when the test has been performed properly, regardless of the presence or absence of anti-Dengue virus antibodies in the specimen on the Dengue Rapid Test. Each Dengue Rapid Test Kit-cassette contains OneStep Dengue RapiCard Insta Test in a foil pouch, a sample buffer, and the product insert. These Dengue Rapid Test Kits-cassette can be shipped in bulk or boxed in 25 or 50 tests.
Besides the Dengue NS1 Rapid Test Kit-strip and the Dengue Rapid Test Kit-cassette, other Dengue Test Kits offered by Diagnostic Automation/Cortez Diagnostics, Inc. include several Dengue ELISA Test Kits. One is a Dengue NS1 ELISA Test, which is a highly sensitive, rapid, and reliable assay that uses a two-step sandwich type immunoassay to detect low levels of NS1 in serum. The other three Dengue ELISA Tests include: Dengue ELISA Test for IgG antibodies, the Dengue ELISA Test for IgM antibodies, and the Dengue ELISA Test for IgG/IgM antibodies in human serum. All above-listed Dengue NS1 Rapid Test, Dengue Rapid Test Kit, Dengue NS1 ELISA Test Kit, and Dengue ELISA Test Kits are readily available for shipment worldwide. Please contact our Customer Service Department for further information on any Dengue NS1 Rapid Test Kit, Dengue Rapid Test, Dengue NS1 ELISA Test Kit, or Dengue ELISA Test Kits.
OTHER RELATED PRODUCTS
USA-based Diagnostic Automation/Cortez Diagnostics, Inc. manufactures and distributes top quality, accurate, safe, and convenient medical testing equipment. Included in this array of medical testing products are various Rapid Tests (HIV Rapid Test, Infectious Disease Rapid Tests, Hepatitis Rapid Tests, Drug Tests, Cancer Rapid Tests, Pregnancy Rapid Tests, and many more). Many of the ELISA Test Kits offered include: Food Allergy ELISA Test Kits, HIV ELISA Tests, Hepatitis ELISA Tests, Parasitology ELISA tests, Blood bank ELISA Tests, and several other ELISA Test Kits). Additional products include: IFA Kits, CIA Test Kits, and Serology Test Kits. Please contact our Customer Service Department for more information on any of these Rapid Tests, ELISA Test Kits, IFA Tests, CIA Test Kits, or Serology Test Kits.
DENGUE ELISA KITS – DENGUE RAPID TESTS – FREQUENTLY ASKED QUESTIONS
Los Angeles-based Diagnostic Automation/Cortez Diagnostics, Inc., a leading manufacturer and distributor of medical test kits in the U.S. and throughout the world, offers a wide variety of Dengue ELISA Kits and Dengue Rapid Tests to its customers. Contained on our web site are frequently asked questions from our customers about Dengue ELISA Kits and Dengue Rapid Tests. Below is a sample of some of those questions that we feel will assist any hospital, clinic, or health organization in making a decision about the proper medical testing equipment for your particular population. Please see the product inserts for more specific information on each product, or contact our Customer Service Department for any further information on these Dengue ELISA Kits, Dengue Rapid Test Kits, or any other medical testing products.
POPULAR DENGUE ELISA KITS – FAQ
Available Dengue ELISA Test Kits:
Dengue Antibody IgG/IgM ELISA Kit (#8115-3)
Dengue Antibody – IgG ELISA Kit (# 8116-3)
Dengue Antibody IgM ELISA Kit (#8117-3)
What serotypes does the Dengue ELISA Kit use?
The Dengue ELISA kits contain micro wells that are coated with purified dengue virus antigen from vero cell cultured type 1-4 dengue.
What is the sensitivity and specificity of the Dengue ELISA Kits?
Dengue IgG/IgM ELISA test is 100% and 78%; Dengue IgG ELISA test is 100% and 96%; Dengue IgM ELISA test is 100% and 86%. See package inserts for details on Dengue ELISA test kits.
Why are 2 wells per sample needed on the Dengue ELISA Kits?
In addition to Dengue recombinant antigen, samples on the Dengue ELISA tests are screened with a normal cell antigen. This step on the Dengue ELISA test optimizes the specificity by helping eliminate any false positives that may occur in the Dengue ELISA test because of non-specific interactions of the antigen with the sample.
How many unknown samples can be tested with the Dengue ELISA Kit?
Up to as many as 44 samples can be tested on the Dengue ELISA Kit.
What is the storage of the Dengue ELISA test Kit?
Storage of Dengue ELISA Kits range from 2 – 8 degrees celsius.
What is the shelf life of the Dengue ELISA test kits?
Storage time of the Dengue ELISA test kits is 12 months from manufacture date.
What is the cpt code of Dengue ELISA kit?
Generally there is a common generic code available for these Dengue ELISA tests– Dengue IgG/IgM ELISA test, Dengue IgG ELISA test, and Dengue IgM ELISA test. For the latest specific code number, please contact our Customer Service Department.
Do you also have Dengue NS1 ELISA kits?
Yes, we offer Dengue NS1 ELISA kit (#8404-25) and Dengue NS1 Rapid Test (#173110-25). Contact our Customer Service Department for more information.
Do you distribute Dengue NS1 Rapid Tests?
We offer a fast and easy-to-use Dengue NS1 Antigen Rapid Test (#173110-25). See our Customer Service Department or product inserts for more information.
CONVENIENT AND EASY-TO-USE DENGUE RAPID TEST – FAQ
Dengue IgG/IgM Antibody Rapid Test (#173106L-25)
What serotypes does the Dengue Rapid Test detect?
This Dengue Rapid Test is a new generation immunochromatographic test using recombinant dengue viral antigens of all four serotypes (DENV 1-4) to detect specific antibody response. By using a mixture of highly purified dengue proteins, the Dengue Rapid Test is able to detect all 4 Dengue serotypes.
What principle is used in the Dengue Rapid Test?
This Dengue Fever Rapid Test is a qualitative immunochromatographic test for the simultaneous detection of IgM and IgG antibodies to dengue virus in human serum, plasma, or whole blood.
How effective is the Dengue Rapid Test in detecting Dengue fever in patients?
A high proportion of dengue virus infections can be detected swiftly and easily using a Dengue Rapid Test. The Dengue Rapid Test can help to diagnose active dengue infections from the very first day fever appears. In facilitating early diagnosis by using this Dengue Rapid Test, therapy and monitoring can begin much earlier, reducing the risk of severe complications.
What is the storage of the Dengue Rapid Test Kit?
Serum/Plasma of the Dengue Rapid Test Kit may be stored at 2 – 8 degrees celsius up to 3 days. For long-term storage, freeze the specimen at -20 degrees celsius for 3 months, or -70 degrees celsius for longer periods. See product insert of Dengue Rapid Test Kit for more details.
What is the total time it takes to process a sample on the Dengue Rapid Test?
Time to process samples on Dengue Rapid Test Kit is approximately 30 minutes. Once the Dengue Rapid Test sample is applied, results can be obtained within 20 minutes.
DENGUE NS1 ELISA KIT and DENGUE NS1 RAPID TEST – FREQUENTLY ASKED QUESTIONS
Dengue fever is a worldwide health problem, and in epidemic proportions in some countries. The World Health Organization estimates that annually, 50-100 million people are infected worldwide with the dengue virus. Studies have shown that early detection of Dengue NS1 virus using tests like the Dengue NS1 ELISA test and Dengue NS1 Rapid test can help in early confirmation, clinical treatment, and control of dengue viral infections. To address this problem, Los Angeles-based Diagnostic Automation/Cortez Diagnostics, Inc., a leading manufacturer and distributor of medical test kits in the U.S. and throughout the world, offers two very popular Dengue NS1 test kits — Dengue NS1 ELISA Kit and Dengue NS1 Rapid Test that detect Dengue NS1 (non-structural protein 1) of Dengue virus. The Dengue NS1 ELISA test and the Dengue NS1 Rapid Test are effective new diagnostic tools for early diagnosis of dengue virus infection. Below are some of the frequently asked questions surrounding these products.
DENGUE NS1 ANTIGEN ELISA KIT – FAQ
Dengue NS1 Antigen ELISA KIT (#8404-25)
Is the Dengue NS1 ELISA kit easy to use?
The Dengue NS1 ELISA Kit is an excellent assay that contains a high level of sensitivity and specificity in detecting the NS1 antigen of Dengue virus. The Dengue NS1 ELISA Kit is a rapid and reliable Dengue ELISA kit that contains sufficient reagents for one plate of 96 wells. The user-friendly Dengue NS1 ELISA Kit contains components that are highly effective and the Dengue NS1 ELISA Kit has easy-to-read controls to determine positive or negative results.
How long is the incubation time of the Dengue NS1 ELISA Kit?
Total incubation time of the Dengue NS1 ELiSA test is approximately 2 hours (60-30-20). Incubation procedures of the Dengue NS1 ELISA test kit are easy to follow. Clear instructions for specific Dengue NS1 ELISA test procedures in specimen collection and preparation are found in the Dengue NS1 ELISA kit product insert which should help with your specific needs.
What is the total time it takes to process a sample on the Dengue NS1 ELISA kit?
Total time for the Dengue NS1 ELISA Test to be performed is approximately 250 minutes.
What principle is used in the Dengue NS1 ELISA test kit?
Dengue NS1 Antigen ELISA test uses the two-step sandwich type immunoassay to detect low levels of NS1 in serum.
What is the storage of the Dengue NS1 ELISA kit?
The samples of the Dengue NS1 ELISA kit may be stored at 2-8 degrees celsius for up to 7 days, or frozen at -20 degrees celsius or lower for up to 30 days. To maintain long-term longevity of the serum on the Dengue NS1 ELISA kit, store at -70 degrees celsius. Avoid repeated freezing and thawing of samples on Dengue NS1 ELISA kit.
What is the shelf life of the Dengue NS1 ELISA kit?
Shelf life of the Dengue NS1 ELISA test is 12 months from the manufacture date.
Do you also distribute Dengue ELISA Kits for IgG and IgM antibodies?
Yes, we offer three Dengue ELISA kits – Dengue ELISA IgG/IgM kit, Dengue ELISA IgG kit, and Dengue ELISA IgM kit. In addition, an easy-to-use and reliable Dengue Rapid Kit is available.
DENGUE NS1 ANTIGEN RAPID TEST – FAQ
Dengue NS1 Antigen Rapid Test (#173110-25)
What is the total time it takes to process a sample on the Dengue NS1 Rapid Test?
Depending on how many samples are collected, testing of Dengue NS1 Antigen Rapid Test should be done immediately after collection of serum. Serum collection and reading results on Dengue NS1 Antigen Rapid Test can be performed in approximately 30 minutes. Caution: do not interpret results of the Dengue NS1 Rapid Test more than 45 minutes after serum collection as this may lead to erroneous results.
What is the storage of the Dengue NS1 Rapid Test?
The sealed pouch and Chase Buffer of the Dengue NS1 Rapid Test are designed to be stored at room temperature (22 – 30 degrees celsius) for the duration of their shelf life. Exposure to temperatures over 30 degrees celsius can impact the performance of the Dengue NS1 Rapid Test and should be minimized.
What principle is used in the Dengue NS1 Antigen Rapid Test?
The Dengue NS1 Antigen Rapid Test is a qualitative, membrane based immunoassay for the detection of NS1 antigen in human serum. The Dengue NS1 Rapid Test membrane is pre-coated with a NS1 specific antibody on the Dengue NS1 Rapid Test line region and utilizes a separate control to assure assay flow and performance. During testing, the Dengue NS1 Antigen Rapid Test sample is added directly to the sample region on the Dengue NS1 Rapid Test and the test is placed into a well containing 3 drops of buffer. The buffer and serum mix and interact with NS1-specific monoclonal antibodies conjugated to gold nano particles. The solution in the Dengue NS1 Rapid Test migrates upward on the membrane to react with the anti-NS1 antibody on the membrane. If NS1 antigen is present, a red line will appear at the Dengue NS1 Rapid Test line.
What is the sensitivity and specificity of the Dengue NS1 Antigen Rapid Test?
The relative high sensitivity in detecting patients with primary infections and the overall high specificity of the Dengue NS1 Antigen Rapid Test make it well suited for the early detection of dengue virus. Recent studies have shown the Dengue NS1 Antigen Rapid Test as having a sensitivity of 85% to 89% and a specificity of nearly 98%. The diagnostic sensitivity of the Dengue NS1 Antigen Rapid Test is significantly enhanced if used in a combination antigen and antibody testing approach.
In addition to its Dengue ELISA Kit for lgG, lgM, and lgG/M antibody detection, Los Angeles based Diagnostic Automation/Cortez Diagnostics, Inc. is pleased to announce a newly arrived Dengue ELISA Kit – Dengue NS1 Antigen ELISA kit which is an Enzyme Linked Immunosorbent Assay for the detection of Dengue virus in human serum even prior to the presence of lgM or lgG antibodies. Dengue NS1 ELISA Kit provides an excellent methodology for specifically detecting NS1 antigen in human serum.
DENGUE NS1 ELISA KIT – PRODUCT FEATURES
The Dengue NS1 ELISA Kit is an excellent assay that contains a high level of sensitivity and specificity in detecting the NS1 antigen of Dengue virus. The Dengue NS1 ELISA Kit is a rapid and reliable ELISA test that contains sufficient reagents for one plate of 96 wells (12 x 8 strips) each. The user friendly Dengue NS1 ELISA Kit contains components that are convenient and easy to use. The Dengue NS1 ELISA Kit has easy-to-read controls to determine positive or negative results. Because of the complications associated with dengue infection, it would be beneficial to have a Dengue NS1 ELISA kit diagnosis early in the illness. Consequently, the Dengue NS1 ELISA Kit is a useful tool for detecting dengue infection during the first few days of fever.
The Dengue NS1 ELISA kit uses one enzymatically amplified, two-step sandwich-type immunoassay to detect low levels of NS1 in serum. In this Dengue NS1 ELISA kit, controls and unknown serum samples are diluted in sample dilution buffer, containing secondary antibody, and incubated in microtitration wells. These wells on the Dengue NS1 ELISA kit have been coated with a highly effective NS1 antibody and then blocked. NS1 antigens present in the samples on this Dengue NS1 ELISA kit are then sandwiched between the capture and secondary antibodies. The presence of NS1 antigen on this Dengue NS1 ELISA kit is confirmed by the colorimetric response obtained using an enzyme-conjugate-HRP and liquid TMB substrate. Once the reaction is stopped on the Dengue NS1 ELISA kit, the enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. The values obtained, on this Dengue NS1 ELISA kit, for the negative and positive sera serve as guidelines as to determining if a sample contains NS1 antigen.
DENGUE NS1 ELISA KIT – TEST RESULTS
Recent studies have confirmed the diagnostic accuracy of Dengue NS1 ELISA Kits for their overall sensitivity to detecting dengue antigens. The sensitivity and specificity of Dengue NS1 ELISA Kits were determined to be good, and the Dengue NS1 ELISA Kit was judged an effective tool for diagnosis of dengue infection, especially within the first four days of illness. Early detection of dengue virus by a Dengue NS1 ELISA test can help in early confirmation and management of this virus. The Dengue NS1 ELISA Kit has the ability to improve the diagnostic algorithm contributing significantly to the clinical treatment and control of dengue viral infections. This Dengue NS1 ELISA Kit would be applicable for both dengue diagnosis and epidemiological studies. Collectively, various studies indicate that tests like this Dengue NS1 ELISA kit deserve inclusion in the diagnostic approach to detection of dengue virus in patients.
The Dengue ELISA Kits are available in two formats: Dengue ELISA Kit for detection of lgG, lgM and lgG/lM antibodies, and Dengue NS1 ELISA Kit for detection of NS1 antigens in human serum. For more information on either the Dengue ELISA Kit or Dengue NS1 ELISA Kit, please contact Customer Service.