Archive for the ‘Parasitology ELISA kits’ Category

Monsters inside Me – Parasite Test Kits Have the Answer

It is a terrifying thought. The television studio that thought up the show by which this article borrows its name had it right. They have cultivated a rather dedicated following and inspired new levels of fear entertainment. The show also begs the question: Could it happen to…

Parasitic infections can happen to anyone. Patients who want—need—to know deserve a fast, accurate answer, and our parasite test kits are here to help. Our toxoplasma test kits, including our immunofluorescence assay variety, are available as ten by eight wells, boast a shelf life of one year, and produce accurate results in approximately 80 minutes. This allows your office to combine affordability and usability to provide your patients with the peace of mind they deserve.

Those who choose our Immunofluorescence Assay kits, give their patients enhanced peace of mind because they incorporate more advanced technology. These kits also arm doctors, PAs, and nurses with images they can show patients. It is used in combination with fluorescence microscopes to identify biomolecules within a cell. With it, dyed target cells are easier to see and reliably identify. This leads to better understanding, more confidence in the treatment, and improved doctor–patient relationships.

Dengue ELISA test

Dengue ELISA test | Rapidtest.com Blog

Have you gotten sick after traveling abroad to areas like Latin America, Southeast Asia, or the Pacific Islands? If you were stung by a mosquito, there is a possibility that you could have been infected with dengue virusDengue infection can be complicated but early recognition and treatments can lower the risks, such as medical complications or death.  Diagnostic Automation / Cortez Diagnostics, Inc., offers quality dengue ELISA tests for your needs. 

Dengue IgG ELISA Test

Typical dengue symptoms are usually high fever, joint pain, bone pain, and rashes. It’s important to watch for any warning signs and go to the emergency room if you are having severe stomach pains, vomiting, red spots, or difficulty breathing. Typically, there have been very few cases in the US, but there was an outbreak in 2001 in Hawaii. When traveling, it’s important to use bug spray and other pest control methods to avoid being bitten. No one wants to travel with this troublesome disease!

Take a look at our dengue ELISA tests and make your purchase today. Whether you are an individual who suspects a problem or are working for a testing lab and need to run this test, Diagnostic Automation / Cortez Diagnostics, Inc., offers excellent tests for your needs. 

Ferritin ELISA Kit blog

Test: Ferritin ELISA Kit

Synonyms: Ferritin EIA

Shelf Life: 12-14 months

Total time: ~ 80 minutes

 

Ferritin elisa kit:

Ferritin is an iron storage protein and a key regulator of iron level in the body with each molecule capable of binding up to 4000 iron atoms. Its main function is storing a soluble and nontoxic form of iron.  Ferritin can store iron and release it in a controlled manner to maintain a balanced level of iron in the body.  Thus ferritin is essential for controlling both iron deficiency and iron overload in the body.

Human gene encoding the major iron storage protein ferritin is FTH1 (heavy polypeptide1).  Defect in ferritin proteins are linked to neurodegenerative diseases [1].

The ideal level of ferritin is 40-60 nanogram/milliliter (ng/ml), below 20ng/ml is deficiency and above 80ng/ml is iron overload.

Ferritin is found in the skeletal muscle (meat), liver, spleen and bone marrow.  A small amount of ferritin is in the blood and that amount of ferritin depicts the iron storage in the body.

Diagnostic Automation offers a rapid and reliable tool as an ELISA kit for measuring the serum concentration of ferritin in blood.

 

 

 

 

Molecular Structure of Human Ferritin [2]

What is Iron deficiency?

Iron deficiency with a complex mechanism is one of the most common nutritional disorders, and at its most extreme it can develop into iron deficiency anemia. Anemia occurs when there is a reduction in the number of red blood cells or red blood cells have a very low level of hemoglobin.  Anemia is common in children and menstruating women typically between ages 14 to 52.  Iron has many functions in human body and one of the most important functions is binding to hemoglobin molecule to serve as an oxygen carrier in every cell of the body [3].

Iron plays fundamental roles in human health, thus maintaining a sufficient supply of iron is crucial.  There are multiple processes through which human body loses iron such as bleeding (especially in menstrual periods in women), sweating, and urination leading to iron deficiency.

Normal function of hemoglobin and multiple enzymes in our body depend on iron.  The absorbance of iron is selective, however our body constantly loses small amount of iron.

It is important to diagnose the underlying cause of anemia since there are multiple factors contributing to anemia such as:

Nutritional deficiency (e.g. vitamin B12 and/or folate)

Genetic anemia such as sickle cell, thalassemia

Cancer

Acute blood loss

Although diagnosis of anemia is easy, but identifying the correct cause of it is crucial as in some anemia patients such as in thalassemia iron overload is the major problem not the deficiency.

Iron is a potent oxidative stressor which can oxidize susceptible cells or component of a cell.  The oxidizing process generates free radicals which can cause substantial damage to the cells and increase the risk of heart diseases by damaging the inner lining cells of the blood vessels or can cause cancer by damaging the DNA of the cells.

At Diagnostic Automation, we offer the Ferritin elisa kit (Ferritin elisa test), a fast (total time ~ 80 minutes) and reliable test to measure the ferritin level in the serum.

In contrast to iron deficiency, there are also iron overload conditions which can cause serious damage to multiple organs.  This increase in iron generally appears in patients who have regular blood transfusions, or have a genetic predisposition to iron overload disorders such as Hemochromatosis (absorbance of too much of dietary iron), Hemolytic anemia, and Hemosiderosis. Hemochromatosis treatment consists of removing blood from the patient to reduce the amount of iron [4].  Monitoring and guided treatment for iron overloads disorders such as hemochromatosis and hemosiderosis are easily done using Diagnostic Automation Ferritin elisa kit.  In addition to these clinical conditions, processed foods fortified with iron are another source of iron overload.  Moreover, high level of ferritin in blood can be indicative of inflammatory disease, liver problem, rheumatoid arthritis, hyperthyroidism, or cancer.

Finally, iron builds strong red blood cells, and ferritin is one of the body’s primary iron-storage proteins.  Thus, ferritin screening is one of the most important proactive and preventive health tests.  At Diagnostic Automation we offer ferritin elisa test (ferritin EIA) as a serum ferritin elisa kit for a rapid, sensitive, and reliable assessment of ferritin level in blood for diagnosis of both iron deficiency and iron overload.

 

 

Ferritin ELISA kit

 

 

Indications for ferritin testing:

 

Ferritin elisa test is performed for two purposes, diagnosing a disorder or monitoring a clinical condition:

 

A.    Diagnosing a disorder

1. Low hemoglobin level

2. Low hematocrit

3. Hemochromatosis

4. Liver diseases

5. Cancers

 

In combination with a total iron binding capacity and iron test a low ferritin test result using ferritin elisa kit can confirm the iron deficiency anemia.

 

B.     Monitoring iron status

  1. Hemochromatosis and hemosiderosis
  2. Pregnant women
  3. Blood donors
  4. Blood transfusion
  5. Renal dialysis patients
  6. Inflammation
  7. Diabetes type II
  8. Hyperthyroidism
  9. Leukemia
  10. Rheumatoid arthritis

 

Intended use of Ferritin ELISA kit Cat # 1601Z:

The Diagnostic Automation Ferritin EIA test is for the quantitative determination of Ferritin concentration in human serum valid for either diagnosing a disorder or monitoring iron status of conditions mentioned above.

 

http://www.rapidtest.com/index.php?i=Ferritin-ELISA-kit&id=40&cat=159

 

 

 

 

Materials and Components

 

Materials provided with Ferritin ELISA Test Kit:

• Antibody-coated ELISA Microplate 96 wells

• Ferritin EIA test reference standard set, contains 0, 10, 50, 100, 400, &

800 ng/ml, (NIBSC-WHO 80/602, human liver standard), ready for use

• Ferritin EIA enzyme conjugate reagent, 12 ml

• TMB Substrate, 12 ml

• Stop Solution, 12 ml

• Ferritin ELISA test wash Buffer Concentrate (50X), 15ml

Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader

 

In addition to Ferritin ELISA kit, Diagnostic Automation offers two additional tests for assessment of iron level:

1. Ferritin CLIA kit (Cat # 9019-16); a Chemiluminescence Immuno Assay detection using Microplate luminometers. The Ferritin CLIA test utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact ferritin molecule.  Ferritin CLIA kit provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.

http://www.rapidtest.com/index.php?i=Ferritin-CLIA-kits—(Chemiluminescence-Immuno-Assay)&id=570&cat=47

 

2. Soluble Transferrin Receptor (sTfR) ELISA kit (Cat # 3126-15) has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia (IDA) and anemia of chronic disease (ACD).  Distinguishing between IDA and ACD is a key step for determining whether iron supplementation would be beneficial.  sTfR ELISA test  is similarly useful for monitoring erythropoiesis in malignancy and chronic renal disease.

 

http://www.rapidtest.com/index.php?i=sTfR-Soluble-Transferrin-Soluble-Receptor-&id=710&cat=159

 

 

 

References:

1. Molecular structure of ferritin:  NCBI

http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=65592

2. Ferritin gene: http://www.ncbi.nlm.nih.gov/gene/2495

3. Polin V et al. Iron deficiency: From diagnosis to treatment. Dig Liver Dis. 2013

4. Crownover BK, Covey CJ. Hereditary hemochromatosis. Am Fam Physician. 2013, 1;87(3):183-90

5. Muñoz M, García-Erce JA, Remacha ÁF. Disorders of iron metabolism. Part II: iron deficiency and iron overload. J Clin Pathol. 2011 Apr;64(4):287-96.

DENGUE IgM ELISA KITS | ELISA KITS

DENGUE IgM ELISA KITS

 

Like other specific Dengue ELISA kits, the Dengue IgM ELISA Kits are enzyme-linked immunosorbent assays for the qualitative identification of antibodies to dengue in human serum.

 

Serological testing is a common method of identifying dengue antibodies, and Dengue ELISA Kits have been found to be some of the most useful methods for bringing about exact serological diagnosis of dengue infection.

 

Because of their sensitivity, capability of handling many samples (96 wells), and potential for automation, Dengue IgM ELISA kits have been taking the place of other procedures that detect IgM antibodies.   Dengue IgM ELISA Kits are quick, easy-to-use, effective in diagnosing patients, and are reliable.  Dengue IgM ELISA kits are highly sensitive assays that detect dengue antibodies.  Sensitivity of Dengue IgM ELISA kits is 86% and Specificity is 100%.  In addition, results of Dengue IgM ELISA Kits can be obtained is less than 30 minutes.

 

PRINCIPLE OF DENGUE IgM ELISA KITS

The principle of Dengue IgM ELISA kits involves three incubation steps (10 minutes each). Purified dengue virus antigen from Vero cell cultured type 1-4 dengue is coated on the microwells before adding patient’s sera at the first incubation procedure.  During the second incubation step, the added enzyme conjugate will bind to any antibodies present.  The third incubation involves the addition of TMB chromogen.  ELISA kit readers can be used to obtain results, or the results can be read visually.

 

NOTABLE FEATURES OF DENGUE IgM ELISA KITS:

  • User-friendly directions and explanation of test procedures
  • Simple and safe reagent preparation
  • Clear instructions on specimen collection
  • Comprehensive package of required materials
  • Explicit quality control and storage guidelines
  • Reliable and easy-to-read test results

See the Dengue IgM Product Page/Insert for more information.  Click here.

Other reliable ELISA Kits in the diagnosis of dengue infection are Dengue IgG ELISA Kits, Dengue IgG/IgM ELISA kits, and Dengue NS1 ELISA kits for detection of NS1 antigens in human serum.  For more information on any of the Dengue ELISA kits or any other ELISA Kits, Parasitology ELISA kits, Rapid Test, IFA Kit, CLIA Test, or Serology Test, please check our website [ here] or contact our Customer Service Department at 818-591-3030.

 

 

Dengue IgM ELISA test kit

FASCIOLA IgG ELISA KIT | ELISA KITS

FASCIOLA IgG ELISA KIT

 

One of the most recognized tests for diagnosis of human fascioliasis is the Fasciola IgG ELISA Test. ** The Fasciola IgG ELISA Kit is the perfect testing instrument for early detection of Fasciolasis infection in humans.  The ELISA test principle is based on the Indirect method where microwells are coated with Fasciola antigen.  Results can be obtained in less than 30 minutes.

The Fasciola IgG ELISA kit is one of the most sensitive assays — reporting a sensitivity of 100% and specificity of 100%.  Additional features of the Fasciola IgG ELISA kit is its simple and safe guidelines for reagent preparation, clear instructions on specimen collection, and reliable quality control and storage directions.  Added to these notable features, is the diagnostic importance of these ELISA kits which can be used at medical facilities around the world.

 

Because Fasciolasis is considered a re-emerging disease in various parts of the world, these ELISA kits are valuable tools in early diagnosis of human fasciolasis in order to establish controls and quickly determine how widespread the disease is in any particular region.

What is Fasciolasis?

Fasciolasis is caused by parasites of the genus Fasciola.  There are two Fasciola species:  Fasciola hepatica and Fasciola gigantic – both can infect humans.

 

Who are most likely to get infected?  Generally, people who live in agriculture regions where livestock, like sheep and cattle, are farmed.  However, infections have historically been found in many parts of the world.  People become infected by eating uncooked watercress or other aquatic vegetation that has been contaminated with the parasite larvae.  Contaminated water is the usual source of transmission also.

What’s the good news?

Fasciolasis is treatable!  Early in the infection, some people show signs of sickness with fever and mild abdominal pain.  Whereas, in the chronic phase these symptoms become more acute, and can include bile duct problems, and an enlarged liver.  The Fasciola IgG ELISA Test can directly aid patients in either stage.   Examination of serum sample by using a Fasciola IgG ELISA kit is one of the fastest and safest ways to aid in diagnosis and subsequent treatment.  Most people respond well to treatment.

 

The Fasciola IgG ELISA kit, like many parasitology ELISA kits, is a test for in-vitro diagnostic use only.  If your health organization is interested in learning more about the Fasciola IgG ELISA kit, or other ELISA test kits, or Rapid Test, CLIA kit, IFA Kit, or Serology Test, please see our full product list on our website home page [click here] or contact our Customer Service Representatives at 818-591-3030.

**Not Sold in the USA.

West Nile Virus ELISA Test | FAQ

WEST NILE ELISA TESTS

Frequently Asked Questions

 

What kinds of West Nile Virus tests are there?

A West Nile ELISA Test is recommended as the best way to confirm presence of the West Nile virus in human serum.  The West Nile ELISA tests are intended for qualitative detection of IgG and IgM antibodies to West Nile virus in blood.

What’s the difference between West Nile IgG ELISA test and West Nile IgM ELISA test?

Early in the infection period virtually all infected persons show signs of IgG antibodies in blood.   The West Nile IgM ELISA Test for IgM antibodies in serum is consistent with acute-phase infection with the West Nile virus.   The West Nile IgM ELISA test may be ordered along with IgG ELISA test, and both may be re-ordered 2-4 weeks later for follow-up analysis.

How does a West Nile ELISA test work?

A West Nile Virus ELISA Test consists of one enzymatically amplified two-step sandwich-type immunoassay.  The West Nile ELISA test kit employes a recombinant antigen called WNRA which can be used as a rapid serological marker for West Nile infection.  A test kit contains sufficient reagents for one plate of 96 wells (12 x 8 strips) each.

What are the notable features of these tests?

West Nile ELISA tests are safe, accurate, and convenient to use.  They have high sensitivity (96%) and specificity (98%) and are cost effective.   West Nile ELISA tests contain easy-to-follow instructions, sufficient materials for specimen collection, and user-friendly test preparation procedures.  Reliable results will be obtained by using precise laboratory techniques and accurately following the package insert.

Can anyone use the West Nile Virus ELISA Test?

If an infection is suspected, a physician can request an analysis of blood from a clinical laboratory. The West Nile ELISA tests are intended for qualitative detection of IgG and IgM antibodies, respectively, to each corresponding kit.  They are not intended to screen blood or blood components.  The West Nile ELISA tests are available for export throughout the world.  In the U.S. the tests are approved for research only.

How long does the test take?

Laboratory testing time for the West Nile ELISA test is approximately 3 hours.

How can I learn more about your West Nile Virus ELISA tests?

Please check our product page for either ELISA test:

Click here for West Nile IgG ELISA test (#8400-25)
Click here for West Nile IgM ELISA test (#8401-25)

 

Our top quality West Nile Virus ELISA kits are available at competitive prices and are shipped quickly to any place around the world.  Customers may also inquire about our other ELISA Tests, or Rapid Tests, Serology Tests, IFA Kits, or medical testing Instrumentation.  Please visit our  Home page, or contact our Customer Service Representatives at 818-591-3030 for more information about these tests, or the convenient and inexpensive West Nile ELISA test kits.