Archive for the ‘Instrumentation’ Category

What is an ELISA reader?


ELISA reader instrument

At Diagnostic Automation we offer an extensive selection of ELISA (enzyme-linked immunosorbent assay) testing kits including, allergy, cancer, diabetes, fertility and infectious disease tests, just to name a few. In short, it is an antibody/antigen test or a test for immune response to things attacking the body such as virus, bacteria and allergens. The test is done in an ELISA plate, also known as a 96-well plate or microplate. Utilizing these tests to detect antibodies/antigens identifies a specific analyte.

ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen/antibody, in a liquid sample or wet sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.

If you are a clinical, biotech or research facility and plan to implement the use of ELISA testing in your organization, you’ll need to invest in an ELISA reader to read the microplates. An ELISA reader measures and quantitates the color differences in the 12 X8 wells of the plate.

ELISA readers or microplate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein.