Archive for the ‘Pregnancy test’ Category

Detecting Pregnancy with HCG Tests

embryo-159691__180A human chorionic gonadotropin (HCG) test is done to check for the presence of HCG in blood or urine. The hormone is produced in the early stages of pregnancy (as early as ten days after conception). Generally, blood tests are more sensitive than urine tests and can therefore be done earlier with the same level of accuracy. If the first test result is negative but a pregnancy is still suspected, the test should be retaken about a week later. Because HCG rises rapidly, a negative result can turn positive within this time period.

Results from the test can also help determine and monitor an abnormality of the pregnancy. For example, low HCG levels during a pregnancy can suggest an ectopic pregnancy or even miscarriage. High HCG levels can indicate twins or triplets.

An HCG test is often used to screen for pregnancy if a woman is about to undergo a medical treatment or test that can potentially harm a fetus, such as an operation or taking certain drugs. HCG tests are designed for rapid determination, so doctors and surgeons can detect a pregnancy even before performing any emergency treatments.

At RapidTest, we offer a range of accurate, fast pregnancy tests in cassette and strip formats.

Future Mothers Need to Know

iStock_000011940368SmallAre you pregnant? Whether it is an unexpected surprise or a sought-after result that begs the question, woman who suspect they are pregnant need to know. They also want to know as soon as possible. After all, this is a huge, life-changing event we all would want to know about sooner rather than later.

To help woman and men answer this question, RapidTest offers safe, reliable, accurate pregnancy tests. These are available in Cassette and Strip options, as well as in-office or at-home varieties. Size options include:

  • 5.0 mm
  • 3.5 mm
  • 2.5 mm

These tests look for the hormone hGC (human chorionic gonadotropin). This is produced by the placenta after it and it’s swimming partner find each other and implant. After about 11 days, often earlier, our kits can dedicate elevated levels of this pregnancy hormone. Most importantly, results with our kits are fast and reliable.

In addition to testing for suspecting women, pregnancy testing is critical essential to providing medical care. Pregnancy affects everything from medication options to surgical procedures and dietary choices. Doctors need to give their patients the best treatment possible, and that starts with one or two of our rapid pregnancy test kits for many women.

Our High-Quality HCG Tests| HCG Rapid Tests

HCG Tests | RapidTest.comPregnancy HCG tests detect the presence of human chorionic gonadotropin (hCG) in either urine or serum. Production of this hormone increases steadily through the first five months of pregnancy, and urine is an easy way to perform the test; however, certain conditions are required.

The test can be performed at any time of day, but a urine sample from first thing in the morning is preferable due to a higher concentration of the hormone. Women should not drink an excessive amount of water to induce urination because it will dilute the sample and make it more difficult to detect the hCG. Additionally, the test should not be performed too early in a woman’s cycle or there is a risk of false negatives.

The hormone hCG is present in in women blood or urine shortly after conception, but it’s more easily detected after embryo implantation. This occurs, on average, within 10 days after conception. Levels of hCG can first be detected by a blood test about 11 days after conception and about 12 – 14 days after conception by a urine test. An hCG level of less than 5mIU/ml is considered negative for pregnancy, and anything above 25mIU/ml is considered positive for pregnancy.

If a pregnancy test shows negative results, repeating it a week later, especially with the morning’s first urine, is likely to be more accurate. Come to Diagnostic Automation / Cortez Diagnostics, Inc. to see the selection of HCG Rapid Tests we have available.

Progesterone ELISA kit blog

Test: Progesterone ELISA kit Cat#: 2077Z

Synonyms: Progesterone EIA kit

Shelf Life: 12-14 months

Total time: ~ 110 minutes

 

Progesterone, a neurosteroid is synthesized from both tissue and circulating cholesterol via pregnenolone through sequential enzymatic actions of desmolase and delta 4-5 isomerase.  Progesterone which regulates a wide range of biological and metabolic functions in the body is the precursor to important steroid hormones such as estrogen, testosterone, and cortisol. It is produced primarily in the corpus luteum of the ovaries in females and testes in males, and in the adrenal glands in both sexes.  Body stores progesterone in adipose tissue.  In blood only 2-10% of the total progesterone circulates as free hormone.   Most of the progesterone in blood is in complex form which is bound to Albumin, Corticosteroid Binding Globulin (CBG), and sex Hormone Binding Globulin (SHBG) [1].

Progesterone’s main function in females is to prepare the endometrium in uterus to accept and sustain the fertilized egg.  It promotes fetal survival and development.  The level of progesterone drops if the egg is not fertilized and menstruation follows. Then progesterone level remains relatively constant throughout the follicular phase of the menstrual cycle. Progesterone concentration increases rapidly following ovulation and remains elevated for 4-6 days and decreases to the initial level 24 hours before the onset of menstruation.  During pregnancy placental progesterone production rises steadily to levels of 10 to 20 times those of the luteal phase peak reaching value as high as 200ng/ml (300-400mg daily) at term [2].

 

 

Progesterone Molecular Structure [3]

 

Progesterone measurements are used to determine ovulation as well as to establish luteal phase defects. Thus progesterone level in blood is useful indicator of ovulation and monitoring luteal function.

At Diagnostic Automation the serum Progesterone ELISA kit offers an accurate prediction of ovulation.

http://www.rapidtest.com/index.php?i=Progesterone-ELISA-kit&id=67&cat=14

Fetal adrenal gland uses large amounts of progesterone supplied by the placenta to make cortisol.  Cortisol is an essential hormone for stress response.  Stress reduces progesterone production as body redirects it into cortisol production to manage and decrease stress level in the body [4& 5].

Recent steroid profiling study offers valuable data for the diagnostics of diseases related to steroidogenesis changes in the fetal and maternal compartments and placenta.  Result showed higher production of active progesterone in fetus with the approaching term [6].

 

Moreover, various studies have demonstrated the supportive role of progesterone on normal development of neurons in the brain.   Following traumatic brain injury progesterone is being utilized as a therapeutic mediator to recover neuron loss and improve cognitive dysfunction.  Multiple mechanisms have been proposed for progesterone role as a neuroprotective agent such as reduction of inflammation following brain trauma, and inhibition of calcium signaling [7&8]. A recent study has proposed progesterone as a candidate to be used as a neuroprotective agent for stroke [9].

 

Progesterone concentration can fluctuate considerably not only from person to person, but also its level varies during day, thus serial measurements are highly recommended.

Measuring progesterone level in blood has been an established method for the detection of ovulation.  Progesterone measurements are used clinically to confirm ovulation and normal function of the corpus luteum in non-pregnant women.

At Diagnostic Automation the serum Progesterone ELISA kit offers an accurate prediction of ovulation.

http://www.rapidtest.com/index.php?i=Progesterone-ELISA-kit&id=67&cat=14

 

 

Indications for progesterone elisa testing:

1. Detecting ovulation

2. Monitoring embryo transfer

3. Monitoring fetal health throughout the high risk pregnancies

4. Identifying high risk patients for abortion early on in pregnancy

5. Checking abnormal bleeding in a non-pregnant woman

6. Diagnosis of ectopic & molar pregnancies

7. Monitoring progesterone replacement therapy

8. Checking normal function of luteum in non-pregnant women

9. Diagnosis of Luteinized unruptured follicle syndrome

10. Detecting ovarian tumors

 

 

Intended use of Progesterone ELISA kit Cat#: 2077Z:

The Diagnostic Automation Progesterone EIA test is for the quantitative determination of Progesterone concentration in human serum valid for detection and monitoring of conditions above.

http://www.rapidtest.com/index.php?i=Progesterone-ELISA-kit&id=67&cat=14

 

 

Materials and Components 

Materials provided with Progesterone ELISA Test Kit:

• Goat Anti-Rabbit IgG-coated Microplate 96 wells

• Progesterone ELISA test Reference Standards: 0, 0.5, 3.0, 10, 25, and 50 ng/ml

Liquids, 0.5 ml each, ready to use

• Rabbit Anti-Progesterone Reagent (pink color), 7 ml

• Progesterone elisa-HRP Conjugate Concentrate (11x), 1.3 ml

• Progesterone elisa-HRP Conjugate Diluent, 13 ml

• Progesterone EIA Control 1, Liquid, 0.5 ml, Ready to use

• Progesterone EIA Control 2, Liquid, 0.5 ml, Ready to use

• TMB Reagent (One-Step), 11 ml

• Stop Solution (1N HCl), 11 ml

 

 

Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled or deionized water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader

 

In addition to progesterone ELISA kit, Diagnostic Automation offers two additional tests for assessment of progesterone level in blood:

17-alpha hydroxy Progesterone ELISA kit (Cat # 1292Z)

Diagnostic Automation 17-Hydroxyprogesterone (17-OH progesterone or 17OHP) elisa kit is expedient for the measurements of levels of 17-OHP which are useful in the evaluation of patients suspected with congenital adrenal hyperplasia.  Since defective enzymes, 21-hydroxylase and 11ß-hydroxilase lead to a buildup of 17OHP level.

http://www.rapidtest.com/index.php?i=17-alpha-hydroxy-Progesterone-ELISA-kit&id=72&cat=14

Progesterone CLIA (Chemiluminescence Immuno Assay) kit (Cat # 9025-16)

Progesterone CLIA detection using Microplate luminometers provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.

http://www.rapidtest.com/index.php?i=Progesterone-CLIA-kit—(Chemiluminescence-Immuno-Assay)&id=535&cat=39

 

 

 

Related kits to progesterone elisa:

 

Beta HCG (Total) ELISA kit (Cat # 4201-16)

Studies have shown that HCG levels can be useful in predicting spontaneous abortions, aiding in the detection of ectopic pregnancy and multiple gestation. Elevated levels of HCG have also been detected in serum from patients with abnormal physiological conditions not related to pregnancy.

http://www.rapidtest.com/index.php?i=Beta-HCG-(Total)-ELISA-kit&id=58&cat=13

 

Luteinizing hormone (LH) ELISA Kit (Cat #4225Z)

This test may be used in conjunction with progesterone elisa kit.  Patients suffering from hypogonadism show increased concentrations of serum LH.  A decrease in steroid hormone production in females is a result of immature ovaries, primary ovarian failure, polycystic ovary disease, or menopause; in these cases, LH secretion is not regulated.

http://www.rapidtest.com/index.php?i=LH-ELISA-kit&id=61&cat=13

 

 

 

 

 

References:

1.Jeremy M Berg, John L Tymoczko, and Lubert Stryer. Biochemistry, 5th edition.2002, New York.  WH. Freeman; ISBN-10: 0-7167-3051-0

2. Hoehn K, Marieb EN. Human Anatomy & Physiology, 2010, San Francisco: Benjamin Cummings; ISBN 0-321-60261-7

3. NCBI

http://pubchem.ncbi.nlm.nih.gov/image/structurefly.cgi?cid=6238

5. Mastorako G, Ilas I. Maternal and fetal hypothalamic-pituitary-adrenal axes during pregnancy and postpartum. Ann N Y Acad Sci. 2003, 997:136-49

6. Hill M, et al. Steroid profiling in pregnancy: A focus on the human fetus, 2013 Apr 10.  J. Steroid Biochem Mol Biol.

7. Pan DS, Liu WG, Yang XF, Cao F (October 2007). Inhibitory effect of progesterone on inflammatory factors after experimental traumatic brain injury. 2007, Biomed. Environ. Sci. 20 (5): 432–8

8. Luoma JI, Stern CM, Mermelstein PG (August 2012). Progesterone inhibition of neuronal calcium signaling underlies aspects of progesterone-mediated neuroprotection. 2012,  J. Steroid Biochem. Mol. Biol. 131 (1-2): 30–6

9. Wong R, Bath PM et al. Progesterone and cerebral ischaemia: the relevance of ageing, 2013 Apr 30. J Neuroendocrinol.

 

 

 

 

Sign of Pregnancy and Pregnancy Symptoms

Diagnostic automation Cortez Diagnostics Inc. offers Home Pregnancy Test (HPT),

OneStep hCG Midstream Urine RapiCard Pregnancy Test

This Pregnancy Test kit is for the determination of hCG (Human Chorionic Gonadotropin) in urine specimens. There is an appearance of hCG in urine soon after conception and its subsequent rapid rise in concentration during early gestational growth.  Pregnancy test is used to obtain a visual, qualitative result for the early detection of pregnancy.

We offer pregnancy tests with two different sensitivities 25mIU/mL and 10mIU/mL:

 The pregnancy tests on the market today range widely in their sensitivity in picking up the pregnancy hormone, hCG.  As a general rule, hCG levels in pregnant women are between 5 – 50mIU a week before your period is due. The hCG levels in your system should double every two to three days.

 The pregnancy test with 10mIU/mL sensitivity – Early Pregnancy test allows one to know earlier if their attempts to conceive were successful and to get medical care as soon as possible. There is a down side to extra sensitive pregnancy tests though. Some non-pregnant women may have low levels of hCG and when they use an extra sensitive pregnancy test the result might show false positive.

 

Warnings: Unites States Surgeon General

Pregnant women should not drink alcohol, may cause birth defect.

 Sign of pregnancy and Pregnancy Symptoms!

Looking for home testing test or for any point of care market place that can give you accurate and fast results?

Visit our website at www.rapidtest.com you will find all different types of pregnancy test we can offer you for:

  • Self testing

  • Over the counter

  • Low price

  • Good sensitivity

  • Blood pregnancy test to detect HCG

Our pregnancy test are CE and FDA approved.

How to test pregnancy?

Our hCG pregnancy tests are easy to use! Click on the below links to view the insert of the pregnancy test you are looking for.

 

OneStep HCG Midstream Urine RapiCard™ InstaTest (HPT) Home Pregnancy Test

OneStep HCG Urine RapiCard™ InstaTest  (Cassette) for Pregnancy Test

OneStep HCG  Urine RapiDip™  InstaTest (2.5mm Strip) for Pregnancy Test

OneStep HCG  Urine RapiDip™  InstaTest (3.5mm Strip) for Pregnancy Test

OneStep HCG Urine RapiDip™  InstaTest (5mm Strip) for Pregnancy Test

OneStep HCG Combo (Urine/Serum) RapiCard™ InstaTest   for Pregnancy Test

OneStep HCG Combo (Urine/Serum) RapiDip™  InstaTest, (3.5mm Strip) for Pregnancy Test

OneStep HCG Combo (Urine/Serum) RapiDip™  InstaTest ,(5mm Strip) for Pregnancy Test