USING HEPATITIS ELISA TEST KITS (HBsAg ELISA KITS) to detect HEPATITIS B VIRUS

HBsAg ELISA KITS

Los Angeles based Diagnostic Automation/Cortez Diagnostics, Inc., a leading provider of Hepatitis Test Kits, offers top quality HBsAg ELISA kits at competitive prices and in a timely manner.  Our company is committed to offering the best quality HBsAg Elisa Kits worldwideThese convenient and easy-to-use HBsAg Elisa Kits (Hepatitis B ELISA Kits) can immediately detect any Hepatitis B surface antigen infection.

 

HBsAg ELISA kits and other Hepatitis ELISA test kits, also known as HBsAg EIA kits and HBV EIA kits, are Enzyme-Linked Immunosorbent Assays for qualitative detection of Hepatitis B viruses in human serum or plasma samples. Hepatitis B ELISA kits (HBsAg Elisa Kits) are intended for screening test of blood donors and as an aid for diagnosis.

 

Hepatitis B virus (HBV) is transmitted through infected body fluids, including blood, semen, and vaginal fluids (including menstrual blood).  It can also be transmitted from a pregnant woman to her child at or near the time of birth.  There are several different Hepatitis ELISA tests kits to detect the hepatitis B virus.  One of the most common Hepatitis ELISA test kits is the Hepatitis B surface antigen (HBsAg) ELISA Test Kit.

The Hepatitis B Test checks for substances in the blood that show whether a hepatitis B infection is active or has occurred in the past.  The Hepatitis ELISA Test Kit (HBsAg ELISA Kit) looks for different signs of infection (markers).  For example, Antigens are markers (HBsAg) made by bacteria or viruses, so the presence of hepatitis B antigens means that the virus is in the body.  Hepatitis B surface antigen (HBsAg) is the earliest sign of an active hepatitis B infection.  This antigen may be present before symptoms of an HBV infection are present.  If this antigen is present for more than 6 months, then there is probably a chronic long-term HBV infection.  This means the Hepatitis B virus can be spread to others through a person’s life.  On the other hand, antibodies (HBsAb) are proteins produced by the body to fight infection.  The presence of Hepatitis B antibodies means that there has been an exposure to the hepatitis B virus, whether from a long past exposure or a current infection.  Genetic material (DNA) of the hepatitis B virus shows that the virus is in the body.  The amount of DNA can help determine how severe the hepatitis infection is and how easily the HBV infection can be spread.  It is important to identify the type of hepatitis virus causing the infection to prevent its spread and choose the proper treatment.

RESULTS OF HBsAg ELISA TESTS

 

For detection of HBsAg, this quick and accurate Hepatitis ELISA test kit uses antibody “sandwich” ELISA method in which, polystyrene microwell strips are pre-coated with monoclonal antibodies specific to HBsAg virus.   Patient’s serum or plasma sample is added to the microwell together with a second antibody conjugated the enzyme horseradish peroxidase (the HRP-Conjugate) and directed against a different epitope of HBsAg.  During incubation period of this HBsAg Elisa Test, the specific immunocomplex formed in case of presence of HBsAg (Hepatitis B virus) in the sample, is captured on the solid phase. After washing to remove sample serum proteins and unbound HRPconjugate, chromogen solutions containing tetramethyl-benzidine (TMB) and urea peroxide are added to the wells. In presence of the antibody-antigen-antibody(HRP) “sandwich” immunocomplex, the colorless chromogens are hydrolyzed by the bound HRP-conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and it is proportional to the amount of antigen captured in the wells, and to its amount in the sample respectively. Wells containing samples negative for the HBsAg virus remain colorless.

Hepatitis B virus (HBV) is an enveloped, double-stranded DNA virus belonging to the Hepadnaviridae family and is recognized as the major cause of blood transmitted hepatitis together with hepatitis C virus (HCV).   HBV virus infection induces a spectrum of clinical manifestations ranging from mild, inapparent disease to fulminant hepatitis, severe chronic liver diseases, which in some cases can lead to cirrhosis and carcinoma of the liver. Classification of a hepatitis B virus infection on this Hepatitis ELISA test requires the identification of several serological markers expressed during three phases (incubation, acute and convalescent) of the infection.

 

EFFECTIVE HEPATITIS TEST KITS

 

Diagnostic Automation/Cortez Diagnostics, Inc. also offers the best quality HbsAg Rapid Test (Strip) RapiDip™ InstaTest to detect Hepatitis B virus.  The One Step Strip Style HBsAg Test (Hepatitis Rapid Test) is a rapid direct binding test for the visual detection of hepatitis B surface antigen (HBsAg) in serum as an aid in the diagnosis of hepatitis B infection.  This One Step HBsAg Test (Hepatitis Rapid Test) is based on the principle of sandwich immunoassay for determination of the HBsAg virus in serum.  Monoclonal and polyclonal antibodies are employed to identify the HBsAg (Hepatitis B virus) specifically. This One Step Hepatitis B test is very sensitive and only takes about 10-20 minutes for the result to be read. Test results are read visually without any instrument.

Customers who use the HBsAg ELISA KITS and HBsAg Rapid Tests also inquire about our HCV ELISA KITS and HCV Rapid Tests that test for the Hepatitis C virus in human serum or plasma.  These widely-used HCV ELISA Test Kits and HCV Rapid Tests are the most convenient and inexpensive HCV ELISA Kits and HCV Rapid Tests on the market today.  For more information about the various Hepatitis ELISA Test Kits or Hepatitis Rapid Test Kits, please contact our Customer Service department.

 

Also available at Diagnostic Automation/Cortez Diagnostics, Inc. are HAV ELISA Test Kits, HCV ELISA Test Kits, HDV ELISA Test Kits, HEV ELISA Test Kits, HGV ELISA Test Kits, and HTLV ELISA Test Kits. 

 

RELATED PRODUCTS

 

Diagnostic Automation/Cortez Diagnostics, Inc. is a USA based company manufacturing and distributing ELISA Test Kits (Autoimmune diseases ELISA Kit, Cardiac Markers, Allergy ELISA kits, HIV ELISA kits, etc.) and Rapid Tests (HIV Rapid Test, Drug Tests or Drugs of Abuse Rapid Tests, Cardiac Rapid Tests, Allergy Rapid Tests, Cancer Rapid Tests, Pregnancy Rapid Tests, Urine Reagent Strips, etc.) and IFA Kits (Autoimmune diseases IFA Kits, Autoimmune Disease Kits, Bacterial Disease IFA Kits, etc.) and Chemilluminescence immuno assays (Infectious Disease kits, Fertility Assays/CLIA kits, Diabetes Assays/CLIA kits, Cardio-Vascular/CLIA kits, Tumor Marker Assays kits, etc.) and ELISA readers – ELISA washers and Serology test kits.

 

Please contact our Customer Service Department for information about any of these ELISA Test Kits, CLIA Kits, IFA Kits, or any other drug testing products of interest.

 

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