Posts Tagged ‘antibodies to hcv’

HCV ELISA kit – HCV ab ELISA Kit – HCV igM ELISA Kit

USING HEPATITIS ELISA TEST KITS

(HCV ELISA KITS) to detect HEPATITIS C VIRUS

 

Los Angeles based Diagnostic Automation/Cortez Diagnostics, Inc., a leading provider of Hepatitis ELISA Test Kits, offers top quality HCV ELISA kits at competitive prices and in a timely manner.  Our company is committed to offering the best quality HCV Elisa kits worldwide.  These convenient and easy-to-use HCV Elisa Kits (Hepatitis C ELISA Test Kits) can immediately detect any Hepatitis C infection.

 

Hepatitis C virus (HCV) is an envelope, single stranded positive sense RNA (9.5 kb) virus belonging to the family of Flaviviridae. Six major genotypes and series of subtypes of HCV have been identified. Isolated in 1989, HCV is now recognized as the major cause for transfusion associated non-A, non-B hepatitis. The disease is characterized with acute and chronic form although more than 50% of the infected individuals develop severe, life threatening chronic hepatitis with liver cirrhosis and hepatocellular carcinomas. Since the introduction in 1990 of anti-HCV screening of blood donations, the incidence of this hepatitis C infection in transfusion recipients has been significantly reduced.

 

There are two kinds of HCV ELISA kits available through Diagnostic Automation/Cortez Diagnostics, Inc.  One easy-to-use HCV ELISA Kit, also known as HCV ab ELISA Kit, is an enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies to hepatitis C virus in human serum or plasma. It is intended for screening blood donors and diagnosing patients related to infection with hepatitis C virus.  The other convenient HCV ELISA Kit is an enzyme-linked immunosorbent assay for qualitative detection of IgM-class antibodies to hepatitis C virus in human serum or plasma. It is intended for clinical diagnosis, management and follow-up of patients related to infection with hepatitis C virus.

 

RESULTS OF HCV ELISA TESTS

 

 

HCV ELISA kit (HCV ab ELISA Kit)

This anti-HCV employs solid phase, indirect ELISA method for detection of antibodies to HCV in two-step incubation procedure. Polystyrene microwell strips are pre-coated with recombinant, highly immunoreactive antigens corresponding to the core and the non-structural regions of HCV (third generation HCV ELISA). During the first incubation step, anti-HCV specific antibodies, if present, will be bound to the solid phase pre-coated HCV antigens. The wells are washed to remove unbound serum proteins, and rabbit anti-human IgG antibodies (anti-IgG) conjugated to the enzyme horseradish peroxidase (HRP-Conjugate) is added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody(IgG) complexes previously formed and the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the antigen-antibody-anti-IgG (HRP) immunocomplex, the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and it is proportional to the amount of antibody captured in the wells, and to the amount of antibody in the sample respectively. Wells containing samples negative for anti-HCV remain colorless.

 

HCV ELISA Kit (HCV IgM ELISA Kit)

This kit employs indirect ELISA assay for detection of IgM antibodies to HCV in two-step incubation procedure. Polystyrene microwell strips are pre-coated with recombinant, highly immunoreactive antigens corresponding to the core and non-structural regions of HCV. During the first incubation step, HCV-IgM specific antibodies, if present, will be bound to the solid phase pre-coated HCV antigens. The wells are washed to remove unbound materials, and then, rabbit anti-human IgM antibodies (anti-IgM) conjugated to horseradish peroxidase (HRP-Conjugate) are added. During the second incubation step, these labeled antibodies will be bound to any antigen-IgM complexes previously formed and the unbound HRP-Conjugat is removed by washing.  Chromogen solutions  containing tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the antigen- antibody-anti-IgM (HRP) immunocomplex; the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for HCV-IgM remain colorless.

 

EFFECTIVE HEPATITIS TEST KITS

 

Diagnostic Automation/Cortez Diagnostics, Inc. also offers the best quality HCV Rapid Tests to detect the Hepatitis C virus.  This One Step Strip Style Anti-HCV Test (Hepatitis C Rapid Test) is a rapid direct binding test for the visual detection of hepatitis C antibodies (anti-HCV).  This Hepatitis C Rapid Test is used as an aid in the diagnosis of hepatitis C infection.   The One Step Hepatitis C Rapid Test is based on the principle of double antigen sandwich immunoassay for determination of anti-HCV in serum.  Purified recombinant antigens are employed to identify anti-HCV specifically.  This One Step Hepatitis C test is very sensitive and only takes 10-20 minutes for the result to be read.  Test results are read visually without any instrument.

Customers who use the HCV ELISA KITS and HCV Rapid Tests also inquire about our Hepatitis B ELISA Test Kits and Hepatitis B Rapid Tests that test for the Hepatitis B virus in human serum or plasma.  These widely-used Hepatitis ELISA Test Kits and Hepatitis Rapid Tests are the most convenient and inexpensive Hepatitis ELISA Test Kits and Hepatitis Rapid Tests on the market today.  For more information about the various Hepatitis ELISA Test Kits or Hepatitis Rapid Test Kits, please contact our Customer Service department.

 

Also available at Diagnostic Automation/Cortez Diagnostics, Inc. are HAV ELISA Test Kits, HBV ELISA Test Kits, HDV ELISA Test Kits, HEV ELISA Test Kits, HGV ELISA Test Kits, and HTLV ELISA Test Kits.

 

RELATED PRODUCTS

 

Diagnostic Automation/Cortez Diagnostics, Inc. is a USA based company manufacturing and distributing ELISA Test Kits (Autoimmune diseases ELISA Kit, Cardiac Markers, Allergy ELISA kits, HIV ELISA kits, etc.) and Rapid Tests (HIV Rapid Test, Drug Tests or Drugs of Abuse Rapid Tests, Cardiac Rapid Tests, Allergy Rapid Tests, Cancer Rapid Tests, Pregnancy Rapid Tests, Urine Reagent Strips, etc.) and IFA Kits (Autoimmune diseases IFA Kits, Autoimmune Disease Kits, Bacterial Disease IFA Kits, etc.) and Chemilluminescence immuno assays (Infectious Disease kits, Fertility Assays/CLIA kits, Diabetes Assays/CLIA kits, Cardio-Vascular/CLIA kits, Tumor Marker Assays kits, etc.) and ELISA readers – ELISA washers and Serology test kits.

 

Please contact our Customer Service Department for information about any of these ELISA Test Kits, CLIA Kits, IFA Kits, or any other drug testing products of interest.