Posts Tagged ‘dengue test kit’

DENGUE NS1 ELISA KIT |DENGUE NS1 RAPID TEST -FREQUENTLY ASKED QUESTIONS

DENGUE NS1 ELISA KIT and DENGUE NS1 RAPID TEST –  FREQUENTLY ASKED QUESTIONS


Dengue fever is a worldwide health problem, and in epidemic proportions in some countries.  The World Health Organization estimates that annually, 50-100 million people are infected worldwide with the dengue virus.   Studies have shown that early detection of Dengue NS1 virus using tests like the Dengue NS1 ELISA test and Dengue NS1 Rapid test can help in early confirmation, clinical treatment, and control of dengue viral infections.  To address this problem,  Los Angeles-based Diagnostic Automation/Cortez Diagnostics, Inc., a leading manufacturer and distributor of medical test kits in the U.S. and throughout the world, offers two very popular Dengue NS1 test kits — Dengue NS1 ELISA Kit and Dengue NS1 Rapid Test that detect Dengue NS1 (non-structural protein 1) of Dengue virus.  The Dengue NS1 ELISA test and the Dengue NS1 Rapid Test are effective new diagnostic tools for early diagnosis of dengue virus infection.  Below are some of the frequently asked questions surrounding these products.

 

DENGUE NS1 ANTIGEN ELISA KIT – FAQ

 

Dengue NS1 Antigen ELISA KIT  (#8404-25)

Is the Dengue NS1 ELISA kit easy to use?

The Dengue NS1 ELISA Kit is an excellent assay that contains a high level of sensitivity and specificity in detecting the NS1 antigen of Dengue virus.  The Dengue NS1 ELISA Kit is a rapid and reliable Dengue ELISA kit that contains sufficient reagents for one plate of 96 wells.  The user-friendly Dengue NS1 ELISA Kit contains components that are highly effective and the Dengue NS1 ELISA Kit has easy-to-read controls to determine positive or negative results.

 

How long is the incubation time of the Dengue NS1 ELISA Kit?

Total incubation time of the Dengue NS1 ELiSA test is approximately 2 hours (60-30-20).  Incubation procedures of the Dengue NS1 ELISA test kit are easy to follow.  Clear instructions for specific Dengue NS1 ELISA test procedures in specimen collection and preparation are found in the Dengue NS1 ELISA kit product insert which should help with your specific needs.

 

What is the total time it takes to process a sample on the Dengue NS1 ELISA kit?

Total time for the Dengue NS1 ELISA Test to be performed is approximately 250 minutes.

 

What principle is used in the Dengue NS1 ELISA test kit?

Dengue NS1 Antigen ELISA test uses the two-step sandwich type immunoassay to detect low levels of NS1 in serum.

 

What is the storage of the Dengue NS1 ELISA kit?

The samples of the Dengue NS1 ELISA kit may be stored at 2-8 degrees celsius for up to 7 days, or frozen at -20 degrees celsius or lower for up to 30 days.  To maintain long-term longevity of the serum on the Dengue NS1 ELISA kit, store at -70 degrees celsius.  Avoid repeated freezing and thawing of samples on Dengue NS1 ELISA kit.

 

What is the shelf life of the Dengue NS1 ELISA kit?

Shelf life of the Dengue NS1 ELISA test is 12 months from the manufacture date.

 

Do you also distribute Dengue ELISA Kits for IgG and IgM antibodies?

Yes, we offer three Dengue ELISA kitsDengue ELISA IgG/IgM kit, Dengue ELISA IgG kit, and Dengue ELISA IgM kit.  In addition, an easy-to-use and reliable Dengue Rapid Kit is available.

 

DENGUE NS1 ANTIGEN RAPID TEST – FAQ

 

Dengue NS1 Antigen Rapid Test  (#173110-25)

 

What is the total time it takes to process a sample on the Dengue NS1 Rapid Test?

Depending on how many samples are collected, testing of Dengue NS1 Antigen Rapid Test should be done immediately after collection of serum.  Serum collection and reading results on Dengue NS1 Antigen Rapid Test can be performed in approximately 30 minutes.  Caution: do not interpret results of the Dengue NS1 Rapid Test more than 45 minutes after serum collection as this may lead to erroneous results.

 

What is the storage of the Dengue NS1 Rapid Test?

The sealed pouch and Chase Buffer of the Dengue NS1 Rapid Test are designed to be stored at room temperature (22 – 30 degrees celsius) for the duration of their shelf life.  Exposure to temperatures over 30 degrees celsius can impact the performance of the Dengue NS1 Rapid Test and should be minimized.

 

What principle is used in the Dengue NS1 Antigen Rapid Test?

The Dengue NS1 Antigen Rapid Test is a qualitative, membrane based immunoassay for the detection of NS1 antigen in human serum.  The Dengue NS1 Rapid Test membrane is pre-coated with a NS1 specific antibody on the Dengue NS1 Rapid Test line region and utilizes a separate control to assure assay flow and performance.  During testing, the Dengue NS1 Antigen Rapid Test sample is added directly to the sample region on the Dengue NS1 Rapid Test and the test is placed into a well containing 3 drops of buffer. The buffer and serum mix and interact with NS1-specific monoclonal antibodies conjugated to gold nano particles.  The solution in the Dengue NS1 Rapid Test migrates upward on the membrane to react with the anti-NS1 antibody on the membrane.  If NS1 antigen is present, a red line will appear at the Dengue NS1 Rapid Test line.

 

What is the sensitivity and specificity of the Dengue NS1 Antigen Rapid Test?

The relative high sensitivity in detecting patients with primary infections and the overall high specificity of the Dengue NS1 Antigen Rapid Test make it well suited for the early detection of dengue virus.  Recent studies have shown the Dengue NS1 Antigen Rapid Test as having a sensitivity of 85% to 89% and a specificity of nearly 98%.  The diagnostic sensitivity of the Dengue NS1 Antigen Rapid Test is significantly enhanced if used in a combination antigen and antibody testing approach.

Dengue Rapid Test, Dengue Fever Rapid Test

Dengue Rapid Tests

 

Los Angeles-based Diagnostic Automation/Cortez Diagnostics, Inc., a leading manufacturer and distributor of Dengue Fever Rapid Tests, offers an easy-to-use Dengue Rapid Test to identify the dengue virus.  This Dengue Fever Rapid Test is a qualitative test for the detection of IgM and IgG antibodies to dengue virus in human serum, plasma or whole blood. The Dengue rapid test provides a differential detection of anti-dengue IgM and anti-dengue-IgG antibodies.  The Dengue Rapid Test can be used for the presumptive distinction between a primary and secondary dengue infection.   A high proportion of dengue virus infections can be detected swiftly and easily using a Dengue Rapid Test.  The Dengue Rapid Test can help to diagnose active dengue infections from the very first day fever appears.  In facilitating early diagnosis by using this Dengue Rapid Test, therapy and monitoring can begin much earlier, reducing the risk of severe complications.  Early and prompt diagnosis with the Dengue Rapid Test can facilitate vector control activities in the community so as to mitigate further transmission.  Also, the expanded use of accurate Dengue Rapid Tests provides important data on the epidemiology and health burden of dengue and in doing so can inform and guide public health policy.

 

According to the World Health Organization’s 2009 Report, “Dengue is the most rapidly spreading mosquito-borne viral disease in the world.  In the last 50 years, incidence has increased 30-fold with increasing geographic expansion to new countries. . . . An estimated 50 million dengue infections occur annually and approximately 2.5 billion people live in dengue endemic countries.”  Because of the vast implications of these findings, it is essential that early detection of dengue fever, like those offered using Dengue Rapid Tests, is vitally important for the prompt care and treatment of infected individuals. Timely, sensitive, and easy to use, the Dengue Rapid Tests are essential for early detection and identification of the infection so that treatment may be started without delay.

 

DENGUE RAPID TEST – NOTABLE FEATURES

 

The Dengue Rapid Test, or OneStep Dengue RapiCardTM InstaTest, is a immunochromatographic assay for the simultaneous detection of IgG and IgM antibodies to Dengue virus in human whole blood, serum or plasma. The assay is used as a screening test for Dengue viral infection and as an aid for differential diagnosis of primary and secondary infections in conjunction with other criteria.  This Dengue Rapid Test is a new generation immunochromatographic test using recombinant dengue viral antigens of all four serotypes to detect specific antibody response. This convenient and easy-to-use Dengue Rapid Test is for in-vitro diagnostic use only.  The Dengue Fever Rapid Test provides an excellent methodology for specifically detecting anti-dengue IgG and IgM antibodies. The presence of high titers of IgG antibodies on the Dengue rapid test does not interfere with the detection of IgM antibodies in the sample.  By using a mixture of highly purified dengue proteins, the Dengue rapid test is able to detect all 4 Dengue serotypes.

Serum, plasma or whole blood samples may be used with this Dengue rapid test. When a specimen is added to the Dengue rapid test, IgG and IgM antibodies in the specimen sample react with blue particles coated with dengue envelope proteins. As this specimen/particle mixture migrates along the length of the Dengue rapid test, the anti-dengue IgG or IgM antibody particle complex is captured by the relevant IgG and/or IgM test bands located in the Dengue rapid test device window causing a pale to dark blue band to form at the IgG or IgM region of the test device window. The intensity of the bands on the Dengue rapid test will vary depending upon the amount of antibody present in the sample. The appearance of any color in a specific Dengue rapid test region (IgG or IgM) should be considered as positive for that particular antibody type (IgG or IgM).  A red procedural control line should always develop in the Dengue rapid test device window to indicate that the Dengue rapid test has been performed properly.

 

DENGUE RAPID TEST – RELATED PRODUCTS

 

Diagnostic Automation/Cortez Diagnostics, Inc. has been a leader in the development of infectious disease rapid tests for more than 20 years.  The use of tests like this Dengue Rapid Test, as a first-line test for the screening and treatment of dengue-infected patients, could help to accelerate the diagnosis of dengue fever in clinical diagnostic laboratories.  Rapid and easy diagnosis of dengue infection using the Dengue Rapid Test can assist patient triage and care management.  This Dengue Fever Rapid Test is available in cassette format only.  For more information on this Dengue Rapid Test, please contact Customer Service.

 

 

Other products are also available in the fight to diagnosis and treat the dengue virus.  One highly effective Dengue Rapid Test is the fairly new Dengue NS1 Rapid Test.  The Dengue NS1 Rapid Test is a chromatographic immunoassay for the qualitative detection of non-structural protein 1 (NS1) antigens in whole blood, serum, or plasma.  Along with this Dengue Rapid Test is the newly arrived Dengue NS1 Antigen ELISA Kit which is an Enzyme Linked Immunosorbent Assay for the detection of dengue virus in human serum even prior to the presence of IgM or IgG antibodies.   Lastly, three Dengue ELISA Kits are available that specifically detect and identify IgG, IgM, and combination IgG/IgM antibodies respectively in human serum.  For more information on any of these Dengue Rapid Tests or Dengue ELISA Kits, please contact our Customer Service Department.