Posts Tagged ‘Parasitology ELISA kits’

Ferritin ELISA Kit blog

Test: Ferritin ELISA Kit

Synonyms: Ferritin EIA

Shelf Life: 12-14 months

Total time: ~ 80 minutes

 

Ferritin elisa kit:

Ferritin is an iron storage protein and a key regulator of iron level in the body with each molecule capable of binding up to 4000 iron atoms. Its main function is storing a soluble and nontoxic form of iron.  Ferritin can store iron and release it in a controlled manner to maintain a balanced level of iron in the body.  Thus ferritin is essential for controlling both iron deficiency and iron overload in the body.

Human gene encoding the major iron storage protein ferritin is FTH1 (heavy polypeptide1).  Defect in ferritin proteins are linked to neurodegenerative diseases [1].

The ideal level of ferritin is 40-60 nanogram/milliliter (ng/ml), below 20ng/ml is deficiency and above 80ng/ml is iron overload.

Ferritin is found in the skeletal muscle (meat), liver, spleen and bone marrow.  A small amount of ferritin is in the blood and that amount of ferritin depicts the iron storage in the body.

Diagnostic Automation offers a rapid and reliable tool as an ELISA kit for measuring the serum concentration of ferritin in blood.

 

 

 

 

Molecular Structure of Human Ferritin [2]

What is Iron deficiency?

Iron deficiency with a complex mechanism is one of the most common nutritional disorders, and at its most extreme it can develop into iron deficiency anemia. Anemia occurs when there is a reduction in the number of red blood cells or red blood cells have a very low level of hemoglobin.  Anemia is common in children and menstruating women typically between ages 14 to 52.  Iron has many functions in human body and one of the most important functions is binding to hemoglobin molecule to serve as an oxygen carrier in every cell of the body [3].

Iron plays fundamental roles in human health, thus maintaining a sufficient supply of iron is crucial.  There are multiple processes through which human body loses iron such as bleeding (especially in menstrual periods in women), sweating, and urination leading to iron deficiency.

Normal function of hemoglobin and multiple enzymes in our body depend on iron.  The absorbance of iron is selective, however our body constantly loses small amount of iron.

It is important to diagnose the underlying cause of anemia since there are multiple factors contributing to anemia such as:

Nutritional deficiency (e.g. vitamin B12 and/or folate)

Genetic anemia such as sickle cell, thalassemia

Cancer

Acute blood loss

Although diagnosis of anemia is easy, but identifying the correct cause of it is crucial as in some anemia patients such as in thalassemia iron overload is the major problem not the deficiency.

Iron is a potent oxidative stressor which can oxidize susceptible cells or component of a cell.  The oxidizing process generates free radicals which can cause substantial damage to the cells and increase the risk of heart diseases by damaging the inner lining cells of the blood vessels or can cause cancer by damaging the DNA of the cells.

At Diagnostic Automation, we offer the Ferritin elisa kit (Ferritin elisa test), a fast (total time ~ 80 minutes) and reliable test to measure the ferritin level in the serum.

In contrast to iron deficiency, there are also iron overload conditions which can cause serious damage to multiple organs.  This increase in iron generally appears in patients who have regular blood transfusions, or have a genetic predisposition to iron overload disorders such as Hemochromatosis (absorbance of too much of dietary iron), Hemolytic anemia, and Hemosiderosis. Hemochromatosis treatment consists of removing blood from the patient to reduce the amount of iron [4].  Monitoring and guided treatment for iron overloads disorders such as hemochromatosis and hemosiderosis are easily done using Diagnostic Automation Ferritin elisa kit.  In addition to these clinical conditions, processed foods fortified with iron are another source of iron overload.  Moreover, high level of ferritin in blood can be indicative of inflammatory disease, liver problem, rheumatoid arthritis, hyperthyroidism, or cancer.

Finally, iron builds strong red blood cells, and ferritin is one of the body’s primary iron-storage proteins.  Thus, ferritin screening is one of the most important proactive and preventive health tests.  At Diagnostic Automation we offer ferritin elisa test (ferritin EIA) as a serum ferritin elisa kit for a rapid, sensitive, and reliable assessment of ferritin level in blood for diagnosis of both iron deficiency and iron overload.

 

 

Ferritin ELISA kit

 

 

Indications for ferritin testing:

 

Ferritin elisa test is performed for two purposes, diagnosing a disorder or monitoring a clinical condition:

 

A.    Diagnosing a disorder

1. Low hemoglobin level

2. Low hematocrit

3. Hemochromatosis

4. Liver diseases

5. Cancers

 

In combination with a total iron binding capacity and iron test a low ferritin test result using ferritin elisa kit can confirm the iron deficiency anemia.

 

B.     Monitoring iron status

  1. Hemochromatosis and hemosiderosis
  2. Pregnant women
  3. Blood donors
  4. Blood transfusion
  5. Renal dialysis patients
  6. Inflammation
  7. Diabetes type II
  8. Hyperthyroidism
  9. Leukemia
  10. Rheumatoid arthritis

 

Intended use of Ferritin ELISA kit Cat # 1601Z:

The Diagnostic Automation Ferritin EIA test is for the quantitative determination of Ferritin concentration in human serum valid for either diagnosing a disorder or monitoring iron status of conditions mentioned above.

 

http://www.rapidtest.com/index.php?i=Ferritin-ELISA-kit&id=40&cat=159

 

 

 

 

Materials and Components

 

Materials provided with Ferritin ELISA Test Kit:

• Antibody-coated ELISA Microplate 96 wells

• Ferritin EIA test reference standard set, contains 0, 10, 50, 100, 400, &

800 ng/ml, (NIBSC-WHO 80/602, human liver standard), ready for use

• Ferritin EIA enzyme conjugate reagent, 12 ml

• TMB Substrate, 12 ml

• Stop Solution, 12 ml

• Ferritin ELISA test wash Buffer Concentrate (50X), 15ml

Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader

 

In addition to Ferritin ELISA kit, Diagnostic Automation offers two additional tests for assessment of iron level:

1. Ferritin CLIA kit (Cat # 9019-16); a Chemiluminescence Immuno Assay detection using Microplate luminometers. The Ferritin CLIA test utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact ferritin molecule.  Ferritin CLIA kit provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.

http://www.rapidtest.com/index.php?i=Ferritin-CLIA-kits—(Chemiluminescence-Immuno-Assay)&id=570&cat=47

 

2. Soluble Transferrin Receptor (sTfR) ELISA kit (Cat # 3126-15) has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia (IDA) and anemia of chronic disease (ACD).  Distinguishing between IDA and ACD is a key step for determining whether iron supplementation would be beneficial.  sTfR ELISA test  is similarly useful for monitoring erythropoiesis in malignancy and chronic renal disease.

 

http://www.rapidtest.com/index.php?i=sTfR-Soluble-Transferrin-Soluble-Receptor-&id=710&cat=159

 

 

 

References:

1. Molecular structure of ferritin:  NCBI

http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=65592

2. Ferritin gene: http://www.ncbi.nlm.nih.gov/gene/2495

3. Polin V et al. Iron deficiency: From diagnosis to treatment. Dig Liver Dis. 2013

4. Crownover BK, Covey CJ. Hereditary hemochromatosis. Am Fam Physician. 2013, 1;87(3):183-90

5. Muñoz M, García-Erce JA, Remacha ÁF. Disorders of iron metabolism. Part II: iron deficiency and iron overload. J Clin Pathol. 2011 Apr;64(4):287-96.

DENGUE IgM ELISA KITS | ELISA KITS

DENGUE IgM ELISA KITS

 

Like other specific Dengue ELISA kits, the Dengue IgM ELISA Kits are enzyme-linked immunosorbent assays for the qualitative identification of antibodies to dengue in human serum.

 

Serological testing is a common method of identifying dengue antibodies, and Dengue ELISA Kits have been found to be some of the most useful methods for bringing about exact serological diagnosis of dengue infection.

 

Because of their sensitivity, capability of handling many samples (96 wells), and potential for automation, Dengue IgM ELISA kits have been taking the place of other procedures that detect IgM antibodies.   Dengue IgM ELISA Kits are quick, easy-to-use, effective in diagnosing patients, and are reliable.  Dengue IgM ELISA kits are highly sensitive assays that detect dengue antibodies.  Sensitivity of Dengue IgM ELISA kits is 86% and Specificity is 100%.  In addition, results of Dengue IgM ELISA Kits can be obtained is less than 30 minutes.

 

PRINCIPLE OF DENGUE IgM ELISA KITS

The principle of Dengue IgM ELISA kits involves three incubation steps (10 minutes each). Purified dengue virus antigen from Vero cell cultured type 1-4 dengue is coated on the microwells before adding patient’s sera at the first incubation procedure.  During the second incubation step, the added enzyme conjugate will bind to any antibodies present.  The third incubation involves the addition of TMB chromogen.  ELISA kit readers can be used to obtain results, or the results can be read visually.

 

NOTABLE FEATURES OF DENGUE IgM ELISA KITS:

  • User-friendly directions and explanation of test procedures
  • Simple and safe reagent preparation
  • Clear instructions on specimen collection
  • Comprehensive package of required materials
  • Explicit quality control and storage guidelines
  • Reliable and easy-to-read test results

See the Dengue IgM Product Page/Insert for more information.  Click here.

Other reliable ELISA Kits in the diagnosis of dengue infection are Dengue IgG ELISA Kits, Dengue IgG/IgM ELISA kits, and Dengue NS1 ELISA kits for detection of NS1 antigens in human serum.  For more information on any of the Dengue ELISA kits or any other ELISA Kits, Parasitology ELISA kits, Rapid Test, IFA Kit, CLIA Test, or Serology Test, please check our website [ here] or contact our Customer Service Department at 818-591-3030.

 

 

Dengue IgM ELISA test kit

FASCIOLA IgG ELISA KIT | ELISA KITS

FASCIOLA IgG ELISA KIT

 

One of the most recognized tests for diagnosis of human fascioliasis is the Fasciola IgG ELISA Test. ** The Fasciola IgG ELISA Kit is the perfect testing instrument for early detection of Fasciolasis infection in humans.  The ELISA test principle is based on the Indirect method where microwells are coated with Fasciola antigen.  Results can be obtained in less than 30 minutes.

The Fasciola IgG ELISA kit is one of the most sensitive assays — reporting a sensitivity of 100% and specificity of 100%.  Additional features of the Fasciola IgG ELISA kit is its simple and safe guidelines for reagent preparation, clear instructions on specimen collection, and reliable quality control and storage directions.  Added to these notable features, is the diagnostic importance of these ELISA kits which can be used at medical facilities around the world.

 

Because Fasciolasis is considered a re-emerging disease in various parts of the world, these ELISA kits are valuable tools in early diagnosis of human fasciolasis in order to establish controls and quickly determine how widespread the disease is in any particular region.

What is Fasciolasis?

Fasciolasis is caused by parasites of the genus Fasciola.  There are two Fasciola species:  Fasciola hepatica and Fasciola gigantic – both can infect humans.

 

Who are most likely to get infected?  Generally, people who live in agriculture regions where livestock, like sheep and cattle, are farmed.  However, infections have historically been found in many parts of the world.  People become infected by eating uncooked watercress or other aquatic vegetation that has been contaminated with the parasite larvae.  Contaminated water is the usual source of transmission also.

What’s the good news?

Fasciolasis is treatable!  Early in the infection, some people show signs of sickness with fever and mild abdominal pain.  Whereas, in the chronic phase these symptoms become more acute, and can include bile duct problems, and an enlarged liver.  The Fasciola IgG ELISA Test can directly aid patients in either stage.   Examination of serum sample by using a Fasciola IgG ELISA kit is one of the fastest and safest ways to aid in diagnosis and subsequent treatment.  Most people respond well to treatment.

 

The Fasciola IgG ELISA kit, like many parasitology ELISA kits, is a test for in-vitro diagnostic use only.  If your health organization is interested in learning more about the Fasciola IgG ELISA kit, or other ELISA test kits, or Rapid Test, CLIA kit, IFA Kit, or Serology Test, please see our full product list on our website home page [click here] or contact our Customer Service Representatives at 818-591-3030.

**Not Sold in the USA.

QUALITY ELISA KITS | INEXPENSIVE

TOP QUALITY ELISA KITS – COMPETITIVE PRICES

 

Customers need the most effective and least expensive ELISA Kits in order to properly serve clients in their own community and around the globe.

Inexpensive ELISA kits are always in demand.  Worldwide, customers request various ELISA kits that specifically suit their geographical area and unique population.  In addition, the requested ELISA Kits need to be simple to use, safe, convenient, and superior in sensitivity and specificity.  ELISA Kits are invaluable tools in treating a vast complex of infections and diseases that ravage a country, city, or small rural area.  Laboratories, doctors’ offices, hospitals, health organizations, forensic labs, and various other health-related institutions, gain by having the best quality ELISA kits at their disposal.   See the partial list of ELISA KITS below.

 

PARTIAL LIST OF MOST POPULARLY-REQUESTED ELISA KITS

AUTOIMMUNE DISEASE  ELISA Kits:
ANA Screen ELISA kit,  Cardiolipin IgG and IgM ELISA kits, and  dsDNA ELISA kit

STEROIDS ELISA Kits:
Cortisol ELISA kit, Estradiol ELISA kit, Progesterone ELISA kit, Free Testosterone ELISA kit, Testosterone ELISA kit, and 17-Alpha Hydroxy Progesterone ELISA kit

THYROID ELISA Kits:
Free T4 and Free T3 ELISA kits, TSH ELISA kit, and T3, and T4 ELISA kits

INFECTIOUS DISEASE ELISA Kits:
Rubella IgG , IgM, and IgA ELISA kits
Chlamydia Trachomatis IgG and IgM ELISA kits
Mycoplasma pneumonia IgG and IgM ELISA kits
Varicella-Zoster IgG and IgM ELISA kits
Brucella IgA, IgG, and IgM ELISA kits
Toxoplasma IgG and IgM ELISA kits
Herpes Simplex 1, IgG and IgM ELISA kits
H. Pylori IgG and IgM ELISA kits

HEPATITIS ELISA Kits:
HIV ELISA kit, HBcAb ELISA kit, HBsAg ELISA kit, HAV IgG and IgM ELISA kits, HCV Ab ELISA kit, and HTLV ELISA kit

PARASITOLOGY ELISA Kits:
Dengue IgG, IgM, and IgG/IgM ELISA kits, Dengue NS1 Antigen ELISA kit, Toxocara ELISA kit, Giardia ELISA kit,  and Cysticercosis (T. Solium) ELISA kit

DIABETES ELISA Kits:
Insulin ELISA kit

FOOD ELISA Kits:
Vitamin H (Biotin) and Vitamin B12 ELISA kits

CARDIAC MARKERS ELISA Kits:
Troponin I ELISA kit,  and C-Reactive Protein (CRP) ELISA kit

FERTILITY ELISA Kits:
FSH ELISA kit, LH ELISA kit, and Prolactin (PRL) ELISA kit

FOR A COMPLETE LIST OF ELISA KITS, VISIT OUR HOME PAGE – CLICK HERE

 

LOW-COST ELISA KITS – RELATED PRODUCTS

If you are shopping around for inexpensive ELISA kits, we ask that you consider Diagnostic Automation/Cortez Diagnostics, Inc. as your main supplier for ELISA Tests, or any other diagnostic medical testing product.  Our company is committed to selling medical diagnostic testing equipment, like ELISA Kits, at fair and competitive prices worldwide.  We will match, or beat, the price you’ve been paying for ELISA Kits elsewhere.   Browse our online catalog of ELISA Kits and see for yourself.   Let us know if you are under any budget constraints.  Our team of ELISA Kit sales specialists will be more than happy to work with you to assemble a low-cost package for your ELISA Kits order.  Check with our Customer Service Department (818) 591-3030 regarding any diagnostic medical testing equipment, including inexpensive Drug Tests, Rapid Tests, IFA Tests, CLIA Tests, Serology Tests, or ELISA Kit Instrumentation.