Posts Tagged ‘Vitamin B12 anemia deficiency’

Ferritin ELISA Kit blog

Test: Ferritin ELISA Kit

Synonyms: Ferritin EIA

Shelf Life: 12-14 months

Total time: ~ 80 minutes

 

Ferritin elisa kit:

Ferritin is an iron storage protein and a key regulator of iron level in the body with each molecule capable of binding up to 4000 iron atoms. Its main function is storing a soluble and nontoxic form of iron.  Ferritin can store iron and release it in a controlled manner to maintain a balanced level of iron in the body.  Thus ferritin is essential for controlling both iron deficiency and iron overload in the body.

Human gene encoding the major iron storage protein ferritin is FTH1 (heavy polypeptide1).  Defect in ferritin proteins are linked to neurodegenerative diseases [1].

The ideal level of ferritin is 40-60 nanogram/milliliter (ng/ml), below 20ng/ml is deficiency and above 80ng/ml is iron overload.

Ferritin is found in the skeletal muscle (meat), liver, spleen and bone marrow.  A small amount of ferritin is in the blood and that amount of ferritin depicts the iron storage in the body.

Diagnostic Automation offers a rapid and reliable tool as an ELISA kit for measuring the serum concentration of ferritin in blood.

 

 

 

 

Molecular Structure of Human Ferritin [2]

What is Iron deficiency?

Iron deficiency with a complex mechanism is one of the most common nutritional disorders, and at its most extreme it can develop into iron deficiency anemia. Anemia occurs when there is a reduction in the number of red blood cells or red blood cells have a very low level of hemoglobin.  Anemia is common in children and menstruating women typically between ages 14 to 52.  Iron has many functions in human body and one of the most important functions is binding to hemoglobin molecule to serve as an oxygen carrier in every cell of the body [3].

Iron plays fundamental roles in human health, thus maintaining a sufficient supply of iron is crucial.  There are multiple processes through which human body loses iron such as bleeding (especially in menstrual periods in women), sweating, and urination leading to iron deficiency.

Normal function of hemoglobin and multiple enzymes in our body depend on iron.  The absorbance of iron is selective, however our body constantly loses small amount of iron.

It is important to diagnose the underlying cause of anemia since there are multiple factors contributing to anemia such as:

Nutritional deficiency (e.g. vitamin B12 and/or folate)

Genetic anemia such as sickle cell, thalassemia

Cancer

Acute blood loss

Although diagnosis of anemia is easy, but identifying the correct cause of it is crucial as in some anemia patients such as in thalassemia iron overload is the major problem not the deficiency.

Iron is a potent oxidative stressor which can oxidize susceptible cells or component of a cell.  The oxidizing process generates free radicals which can cause substantial damage to the cells and increase the risk of heart diseases by damaging the inner lining cells of the blood vessels or can cause cancer by damaging the DNA of the cells.

At Diagnostic Automation, we offer the Ferritin elisa kit (Ferritin elisa test), a fast (total time ~ 80 minutes) and reliable test to measure the ferritin level in the serum.

In contrast to iron deficiency, there are also iron overload conditions which can cause serious damage to multiple organs.  This increase in iron generally appears in patients who have regular blood transfusions, or have a genetic predisposition to iron overload disorders such as Hemochromatosis (absorbance of too much of dietary iron), Hemolytic anemia, and Hemosiderosis. Hemochromatosis treatment consists of removing blood from the patient to reduce the amount of iron [4].  Monitoring and guided treatment for iron overloads disorders such as hemochromatosis and hemosiderosis are easily done using Diagnostic Automation Ferritin elisa kit.  In addition to these clinical conditions, processed foods fortified with iron are another source of iron overload.  Moreover, high level of ferritin in blood can be indicative of inflammatory disease, liver problem, rheumatoid arthritis, hyperthyroidism, or cancer.

Finally, iron builds strong red blood cells, and ferritin is one of the body’s primary iron-storage proteins.  Thus, ferritin screening is one of the most important proactive and preventive health tests.  At Diagnostic Automation we offer ferritin elisa test (ferritin EIA) as a serum ferritin elisa kit for a rapid, sensitive, and reliable assessment of ferritin level in blood for diagnosis of both iron deficiency and iron overload.

 

 

Ferritin ELISA kit

 

 

Indications for ferritin testing:

 

Ferritin elisa test is performed for two purposes, diagnosing a disorder or monitoring a clinical condition:

 

A.    Diagnosing a disorder

1. Low hemoglobin level

2. Low hematocrit

3. Hemochromatosis

4. Liver diseases

5. Cancers

 

In combination with a total iron binding capacity and iron test a low ferritin test result using ferritin elisa kit can confirm the iron deficiency anemia.

 

B.     Monitoring iron status

  1. Hemochromatosis and hemosiderosis
  2. Pregnant women
  3. Blood donors
  4. Blood transfusion
  5. Renal dialysis patients
  6. Inflammation
  7. Diabetes type II
  8. Hyperthyroidism
  9. Leukemia
  10. Rheumatoid arthritis

 

Intended use of Ferritin ELISA kit Cat # 1601Z:

The Diagnostic Automation Ferritin EIA test is for the quantitative determination of Ferritin concentration in human serum valid for either diagnosing a disorder or monitoring iron status of conditions mentioned above.

 

http://www.rapidtest.com/index.php?i=Ferritin-ELISA-kit&id=40&cat=159

 

 

 

 

Materials and Components

 

Materials provided with Ferritin ELISA Test Kit:

• Antibody-coated ELISA Microplate 96 wells

• Ferritin EIA test reference standard set, contains 0, 10, 50, 100, 400, &

800 ng/ml, (NIBSC-WHO 80/602, human liver standard), ready for use

• Ferritin EIA enzyme conjugate reagent, 12 ml

• TMB Substrate, 12 ml

• Stop Solution, 12 ml

• Ferritin ELISA test wash Buffer Concentrate (50X), 15ml

Materials required but not provided:

• Precision pipettes: 5~40μl, and 0.05~0.2ml

• Disposable pipette tips

• Distilled water

• Vortex mixer or equivalent

• Absorbent paper or paper towel

• Graph paper

• ELISA kit Microplate Washer

• ELISA kit Microplate Reader

 

In addition to Ferritin ELISA kit, Diagnostic Automation offers two additional tests for assessment of iron level:

1. Ferritin CLIA kit (Cat # 9019-16); a Chemiluminescence Immuno Assay detection using Microplate luminometers. The Ferritin CLIA test utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact ferritin molecule.  Ferritin CLIA kit provides a sensitive, high throughput, and economical alternative to conventional colorimetric methodologies.

http://www.rapidtest.com/index.php?i=Ferritin-CLIA-kits—(Chemiluminescence-Immuno-Assay)&id=570&cat=47

 

2. Soluble Transferrin Receptor (sTfR) ELISA kit (Cat # 3126-15) has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia (IDA) and anemia of chronic disease (ACD).  Distinguishing between IDA and ACD is a key step for determining whether iron supplementation would be beneficial.  sTfR ELISA test  is similarly useful for monitoring erythropoiesis in malignancy and chronic renal disease.

 

http://www.rapidtest.com/index.php?i=sTfR-Soluble-Transferrin-Soluble-Receptor-&id=710&cat=159

 

 

 

References:

1. Molecular structure of ferritin:  NCBI

http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=65592

2. Ferritin gene: http://www.ncbi.nlm.nih.gov/gene/2495

3. Polin V et al. Iron deficiency: From diagnosis to treatment. Dig Liver Dis. 2013

4. Crownover BK, Covey CJ. Hereditary hemochromatosis. Am Fam Physician. 2013, 1;87(3):183-90

5. Muñoz M, García-Erce JA, Remacha ÁF. Disorders of iron metabolism. Part II: iron deficiency and iron overload. J Clin Pathol. 2011 Apr;64(4):287-96.

VITAMIN B12 ELISA KITS | ANEMIA ELISA KITS | VITAMIN B12 DEFICIENCY

VITAMIN B12 ELISA KITS

 

VITAMIN B12 DEFICIENCY

Vitamin B12 plays an important role in the functioning of a healthy body.  It is essential to the production of red blood cells that carry oxygen through the body’s tissue, including a healthy nervous system.

Two groups of individuals that are most prone to Vitamin B12 deficiency are the elderly and vegans. The ability of older adults to absorb and digest water soluble vitamins (like Vitamin B12) decreases with age, and the diet of vegans lacks B12 found in animal meat.

BENEFITS OF VITAMIN B12 ELISA KITS

Common Vitamin B12 deficiency is pernicious anemia, which is caused by poor Vitamin B12 absorption – when the stomach makes less of the substance the body needs to properly absorb vitamin B12.  Vitamin B12 ELISA kits have been shown to be the best assay in measuring B12 in serum.  Vitamin B12 ELISA kits are delayed competitive enzyme immunoassays for the in vitro quantitative detection of vitamin B12 in human serum or plasma.

Categorized as Anemia ELISA kits, these Vitamin B12 ELISA tests have high sensitivity and excellent specificity for detection of human Vitamin B12 in serum.  The sensitivity of the Vitamin B12 ELISA kit is 70.13 pg/ml.  Vitamin B12 ELISA kits are user-friendly, simple, and safe.  Results are quick, reliable, and easy to read.  Total time for test procedure is approximately 95 minutes.  For more information on the Vitamin B12 ELISA kit, click [here].

 

Vitamin B12 ELISA kit

 

RELATED ELISA KIT PRODUCTS

Another ANEMIA ELISA kit is one that detects Iron deficiency.  The Ferritin ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative determination of ferritin in human serum.  The Ferritin ELISA kit, like other ELISA kits, is for in-vitro diagnostic use only. The sensitivity of the Ferritin ELISA kit is 5.0 ng/ml and the specificity is 98.7%.  Click [here] for Ferritin ELISA kit product details.

Other related ELISA kits are Vitamin B12 ELISA kits (in Food) which are enzyme immunoassays for the quantitative determination of Vitamin B12 in Food.  The sensitivity is 0.3 ng/ml and the specificity is 98%.  Click [here] for product details.

Product information on the Vitamin B12 ELISA Kits, Ferritin ELISA kits, or any other ELISA Kits, can be obtained from our main website product page [click here].

LosAngeles-based Diagnostic Automation/Cortez Diagnostics, Inc. prides itself on offering simple, convenient, and inexpensive ELISA Kits worldwide.   For more information on these ELISA Kits, or any other diagnostic medical testing equipment, including Rapid Tests, IFA Kits, CLIA test kits, Serology, and Instrumentation, please contact our Customer Service Representatives at 818-591-3030.