Testosterone Saliva Elisa kit description:

Testosterone Saliva ELISA kit is for the quantitative determination of Testosterone by enzyme immunoassay in human saliva. For in vitro diagnostic use only.





Materials Provided with Testosterone Saliva ELISA Kit:
1. Testosterone EIA Plate: coated Rabbit Anti-Testosterone Antibody
2. Testosterone-Biotin Conjugate
3. Streptavidin-HRP Conjugate
4. Testosterone Saliva Calibrators
5. Controls: testosterone in a protein-based buffer
6. Biotin Conjugate Assay Buffer
Contents: containing a protein-based buffer
7. HRP Conjugate Assay Buffer
8. Wash Buffer 10x
9. TMB Substrate
10. Stopping Solution

Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Testosterone Saliva ELISA Assay Kit Background Information:
Testosterone is a C-19 steroid secreted from the testis and the adrenal cortex in men and from the adrenal cortex and ovary in women. Testosterone measurements are used mainly for clinical evaluation of hypogonadism in males and hyperandrogenic states in females. Most of the circulating testosterone is bound to three proteins: sex hormone binding globulin (44-78%), albumin (20-54%) and cortisol binding globulin (small amount). Only about 2-3% of the total circulating testosterone remains unbound or in the free form. Only the free portion (or the non-SHBG bound fraction) of the circulating testosterone is thought to be available to tissues where it exerts its biological actions. The salivary hormone assays are advocated for their noninvasive, easy sample collection method. Salivary testosterone is of great clinical value for it represents a filtered fraction of plasma testosterone and is independent of flow rate. Many studies have suggested that salivary testosterone correlates well with either free or non-SHBG bound testosterone.




Testosterone Saliva ELISA Test Principle:
The principle of the following enzyme immunoassay test follows a two-step competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and a biotinlabelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. For additional details please refer to the instructions for use.



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