Myoglobin ELISA Test description:

Diagnostic Automation, Inc. offers a Myoglobin ELISA test kit which is an Enzyme Immunoassay for the quantitative determination of Myoglobin concentration in human serum. The Myoglobin ELISA kit provides a rapid, sensitive, and reliable assay for the quantitative measurement of myoglobin in serum. The antibodies developed for the test will determine a minimal concentration of 5.0ng/ml with no cross-reactivity with related cardiac or skeletal enzymes.





Materials provided with Myoglobin ELISA test kits:
1. Anti-myoglobin murine monoclonal coated Microplate
2. Myoglobin ELISA Test Reference Standard Set:
0, 25, 100, 250, 500& 1000 ng/ml
3. Myoglobin EIA Kit cTnI Enzyme HRP-Conjugate Reagent
4. TMB Reagent
5. Myoglobin ELISA Test Kit Stop Solution

Materials & Instrumentations required, not included:
1. Distilled water
2. Precision pipettes
3. EIA Kit Microplate Washer
4. EIA Kit Microplate Reader at 450nm





Myoglobin ELISA Kit Background Information
Myoglobin, a heme protein with a molecular weight of approximately 17,500 Daltons is found in both cardiac and skeletal muscle. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum. Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours.
In the absence of skeletal muscle trauma or other factors associated with a non-cardiac related increase in circulating myoglobin, its levels have been used as an early marker for myocardial infarct. A number of reports suggest using the measurement of myoglobin as a diagnostic aid in ruling out myocardial infarction with negative predictive values of up to 100% reported at certain time periods after the onset of symptoms. Unlike the other cardiac enzymes such as creatine kinase and the MB isoform (i.e., CK and CK/MB) which do not reach serum levels until several hours post-infarction (approx. 19 hours), myoglobin levels can be expected to peak within 6 to 9 hours.



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Myoglobin Elisa Test Principle
The Myoglobin ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the myoglobin molecule. Mouse monoclonal anti-myoglobin antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-myoglobin antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the two antibodies, resulting in the myoglobin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After 45 minute incubation and then washing, TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of myoglobin is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.





Myoglobin elisa kit Performance Characteristics:
Clinical Performance
A clinical investigation was conducted to determine the accuracy of the Myoglobin ELISA kit as compared to the Abbott AxSym Myoglobin MEIA. The data is presented below:
A statistical study using 150 clinical patient serum samples, ranging in myoglobin concentration from 3.7 ng/ml to 919.8 ng/ml as analyzed using the Myoglobin ELISA kit (13.0 ng/ml to 1011.0 ng/ml Abbott Myoglobin MEIA), demonstrated equivalent correlation with the AxSym Myoglobin kit as shown in data insert/clinical performance.
Correlation coefficient = 0.9392
Slope = 0.8871
Intercept = 55.051
Mean = 287.9 ng/ml
Abbott Myoglobin Mean = 262.5 ng/ml
Hook Effect
No high-dose hook effect is observed in this test with patient sample concentrations up to
10,000ng/ml.

Recovery
Various patient serum samples of known myoglobin levels were combined and assayed in
duplicate. The mean recovery was 102.8%.

LinearityThree patient samples were serially diluted to determine linearity. The mean recovery was 105.8%.




Related cardiac ELISA test kits:

Troponin ELISA kit
http://www.rapidtest.com/index.php?i=Troponin-ELISA-kit&id=46&cat=10

CK-MB ELISA kit
http://www.rapidtest.com/index.php?i=CK-MB-ELISA-kit&id=44&cat=10

Lp(a) ELISA kit
http://www.rapidtest.com/index.php?i=Lp(a)-ELISA-test-kit&id=198&cat=10

CRP ELISA kit
http://www.rapidtest.com/index.php?i=C-Reactive-Protein-(CRP)-ELISA-kit&id=47&cat=10

Digoxin ELISA kit
http://www.rapidtest.com/index.php?i=Digoxin-ELISA-kit&id=43&cat=10

Diagnostic Automation Inc. also provides other Cardiac markers ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.