HIV 1,2 Ab/Ag ELISA kit description:

"Export Use Only"
Diagnostic Automation offers an HIV 1, 2 Ag/Ab ELISA or EIA kit, which is an enzyme-linked immunosorbent assay for the qualitative detection of antigens or antibodies to Human Immunodeficiency Virus (HIV) type 1 and/or type 2 in human serum or plasma. The HIV 1, 2 Ag/Ab ELISA kit is also known as 4th generation ELISA for HIV detection. The HIV 1, 2 Ag/Ab ELISA assay kit as in other Blood Bank ELISA Kits is designed as an aid for screening of blood donors, and diagnosis of clinical conditions related to infection with HIV-1 and/or HIV-2. The Sensitivity of the HIV 1, 2 Ag/Ab elisa assay Kit has high sensitivity and specificity, and involves a simple operation procedure.




Materials Provided with HIV 1, 2 Ag/Ab Elisa kit:
1. Microwell strips: wells contain recombinant HIV 1/2 antigens & anti-p24 antibodies
2. HIV 1/2 Elisa test Negative Control
3. HIV 1/2 Elisa test Positive Control-1 (HIV 1)
4. HIV 1/2 Elisa test Positive Control-1 (HIV 2)
5. Antigen Positive Control:HIV p24 recombinant antigen
6. HIV 1/2 antigen HRP-Conjugate Reagent: HRP-conjugated avidin
7. Biotin-Conjugate Reagent:Biotinylated anti-HIV p24 antibodies
8. Stock Wash Buffer
9. Diluent concentrates 20x
10. Chromogen Solution A
11. Chromogen Solution B
12. Stop Solution
13. Plastic sealable Bag, 1unit
14. Cardboard Plate Cover, 2sheets
15. Package Inserts

Materials & Instrumentations Required not provided
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




HIV 1, 2 Ag/Ab Elisa kit Background Information:
HIV-1 was thought to be the only cause of these syndromes until 1986, when a second type of Human Immunodeficiency Virus (HIV-2) was isolated and also reported to cause AIDS (acquired immunodeficiency syndrome). Both viruses have the same morphology and lymphotropism, and the modes of transmission appear to be identical. In addition, HIV-1 and HIV-2 genomes exhibit about 60% homology in conserved genes such as gag and pol. Serologic studies have also shown that the core proteins of HIV-1 and HIV-2 display frequent cross-reactivity whereas the envelope proteins are more type-specific. Despite this immunologic cross-reactivity, detection of antibodies to HIV-2 with any of the licensed HIV-1 enzyme immunoassays is highly variable. This HIV-1/HIV-2 ELISA kit was developed to detect antibodies to HIV-1 and /or HIV-2, for blood screening and diagnostic purposes. The first HIV ELISA tests were commercially available in 1985 and since then three more generations of tests have been established. First and second generation HIV ELISA kits were based on indirect ELISA. Only through an enzyme-labeled anti-human IgG antibody, could they identify IgG antibodies however, third generation HIV ELISA kits which uses a different method which can identify all antibodies, for example IgG and IgM. Third generation HIV ELISA kits greatly increased the ELISA test's sensitivity compared to previous first and second generation. These HIV elisa test kits used a double antigen sandwich method where antibodies were identified because the solid phase polystyrene plates were coated with antigens, and with the aid of another enzyme-labeled antigen. The third generation HIV ELISA kits would identify antibodies in a much shortened window period. Detection was 11 days sooner than second generation for detection of IgM antibodies which are only present during the early stages of infection. Fourth generation HIV ELISA kits further reduced the window period of antibody identification. Since becoming commercially available in 1998, fourth generation HIV ELISAs can detect HIV antigens (p24) and antibodies simultaneously. Thus, with the identification of HIV antigens (p24), these new HIV ELISA kits shortened the window period to 16 days. As a result, HIV infection can be detected 8 days earlier than the previous third generation ELISA tests, making HIV 1, 2 Ab/Ag Elisa Assay kit an important blood screening tool and clinical diagnosis worldwide.





HIV 1, 2 Ab/Ag Elisa Assay Principle
The principle of the HIV 1 & 2 Ab/Ag ELISA kit is a two-step incubation, sandwich enzyme immunoassay. In this assay, anti-HIV (p24) antibodies are pre-coated on the polystyrene microwell strips along with recombinant HIV antigens. Before the first incubation stage, biotinylated anti-HIV (p24) antibodies are added to the wells, together with the patient's serum/plasma sample. If any specific HIV 1/2 antibodies are present in the sample, they will be captured inside the wells at the time of first incubation. At the same time, HIV p24 antigen (if present) will be captured. This capture is considered a double antibody sandwich complex which is comprised of the coated antibodies-p24 bioitinylated antibodies. After this, unbound serum proteinsare washed and during the second incubation stage, enzyme Horseradish Perioxidase (HRP) is added in order to detect the captured HIV p24 antigen-biotinylated antibody complex (or HIV 1/2 antibodies). The HRP has been conjugated to second HIV 1 & 2 recombinant antigens and to avidin.
Detection of p24: After p24 has been captured inside the wells, avidin will react with the biotin and attach the HRP to the Ab-p24-Ab complex. Detection of HIV 1/2 antibody: After HIV 1/2 antibodies have been captured inside the wells, these captured antibodies will bind to the HRP-conjugated antigens, forming Ag-Ab-Ag(HRP) sandwich immunocmplex. After these stages, the unbound conjugate is removed by washing the microwells. Added to the wells at this point are the chromogen solutions. A blue-colored product is produced when the chromogens are hydrolyzed by the bound HRP in wells containing the Ag-Ab-Ag (HRP) and/or Ab-per-Ab(HRP) sandwich immunocomplexes. After stopping the reaction, the blue color turns yellow. The color intensity is proportional to the amount of antibodies or p24.




Notable features of HIV 1, 2 Ag/Ab elisa assay kit:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results


HIV 1, 2 Ag/Ab elisa Test Results Interpretations:
(S = the individual absorbance (OD) of each specimen)
Negative Results (S/C.O. <1): Samples giving absorbance less than the Cut-off value are negative for this assay, which indicates that no HIV 1/2 antibodies or p24 antigen have been detected with this HIV (1+2) Ag&Ab ELISA kit
Positive Results (S/C.O. 1): Samples giving an absorbance equal to or greater than the Cut-off
value are considered initially reactive, which indicates that HIV 1/2 antibodies and/or p24 antigen have probably been detected using this HIV (1+2) Ag&Ab ELISA kit. For additional details please refer to the instructions for use.


HIV 1,2 Ab/Ag ELISA test assay Related kits:


HIV 1/2 Elisa Related Kits: Cat # 1516-12

http://www.rapidtest.com/index.php?i=HIV-1,2-ELISA-Test-(CE-certified)&id=165&cat=122

HIV 1/2 Rapid Test (Serum, Plasma,WB) Cat # 177575-1-12

http://www.rapidtest.com/index.php?i=HIV-1/2-Rapid-Test-(Serum,WB,Plasma)-&id=385&cat=124


Diagnostic Automation Inc. also provides other Test Kits:

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.


* Not to be sold, shipped or distributed in The United States.