T3 Uptake ELISA Kit
| Name |
T Uptake ELISA Test |
|---|---|
| Full name |
Human T3 Uptake ELISA Test Kit |
| Category Name | Thyroid ELISA kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | Competitive ELISA |
| Detection Range | 18 - 47% Uptake |
| Sample | 50 uL |
| Specificity | 96.80% |
| Sensitivity | 0.4 ug/dl |
| Total Time | ~75 min |
| Shelf Life | 12 Months from the manufacturing date |
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T3 Uptake ELISA Kit description:
The Diagnostic Automation T3 Uptake ELISA is intended for the Measurement of the Total Amount of Binding Sites Available for the Thyroid Hormones in Human Serum or Plasma by a Microplate Enzyme Immunoassay.
Material Provided with T3 Uptake ELISA Kit:
1. Human Serum References: Four human serum based reference standards
2. T3 Uptake Enzyme Reagent: triiodothyronine -HRP conjugate
3. Microplate: sheep anti-thyroxine serum coated wells
4. T3-Uptake Conjugate Buffer
5. Wash Solution Concentrate
6. Substrate A: TMB in buffer
7. Substrate B: H2O2 in buffer
8. Stop Solution: 1N HCl
9. Product Instructions
Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm
T3 Uptake ELISA Kit Background Information:
Thyroid gland under the regulatory control of thyrotropin hormone secretes thyroxine (T4) and triiodothyronine (T3) into the general circulation. The released hormones do not circulate as free molecules but are almost entirely (99.9%) bound to specific serum proteins. Since the metabolic processes are regulated entirely by the concentration of the free thyroid hormones, which are inversely related to the levels of the binding proteins, an assessment of the binding capacity
of human serum was developed.
T3 Uptake ELISA Test Principle:
The T3 Uptake EIA test is based on the principle of a solid phase enzyme-linked Competitive Enzyme Immunoassay. The required components for assessing the binding capacity of human serum are enzyme-T3 conjugate, thyroxine, binding protein (P), and immobilized thyroxine antibody (Ab). Upon mixing the enzyme-conjugate and thyroxine with the specimen, a binding reaction results between the patient's binding proteins and the added thyroxine but not with the enzyme conjugate. For additional details please refer to the instructions for use.
Diagnostic Automation Inc. also provides other Thyroid ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .