Mycobacterium Tuberculosis (TB) IgG ELISA Test description:

Diagnostic Automation Inc. Mycobacterium Tuberculosis ELISA Kit is an Enzyme immunoassay (EIA) for the detection and quantitative determination of human IgG antibodies against Mycobacterium Tuberculosis in human serum and plasma. Further applications of this elisa kit in other body fluids are possible and can be requested from our Technical Service at 818-591-3030.


Materials Provided with Mycobacterium Tuberculosis ELISA Test Kit:

1. Mycobacterium Tuberculosis EIA Kit Microtiter Strips: 12 strips with 8 breakable wells, coated with a M. tuberculosis antigen mixture(recombinant Mycobacterium tuberculosis antigens: 18, 36 & 40 kDa)
2. Mycobacterium Tuberculosis IgG ELISA Kit Calibrator A (Negative Control), 2 ml
no IgG antibodies
3. Mycobacterium Tuberculosis IgG ELISA Kit Calibrator B (Cut-Off Standard), 2 ml
low concentration of IgG antibodies against Mycobacterium tuberculosis
4. Mycobacterium Tuberculosis IgG ELISA Kit Calibrator C (Weak Positive Control), 2 ml, medium concentration of IgG antibodies against Mycobacterium tuberculosis
5. Mycobacterium Tuberculosis IgG ELISA Test Kit Calibrator D (Positive Control), 2 ml
high concentration of IgG antibodies against Mycobacterium tuberculosis
6. Influenza A IgG Eia Test Enzyme Conjugate, 15 ml
anti-human-IgG-HRP (rabbit), in protein-containing buffer solution
7. Mycobacterium Tuberculosis IgG Eia Test Kit Substrate, 15 ml
8. Stop Solution, 15 ml
9. Sample Diluent, 60 ml
10. Mycobacterium Tuberculosis IgG Test Washing Buffer Washing Buffer, 60 ml
11. Plastic Foils, 2 pieces to cover the microtiter strips during incubation
12. Plastic Bag, Resealable for the dry storage of non-used strips



Materials & Instrumentations required not included:

1. Reagent tubes for the serum dilution
2. Bidistilled water
3. Precision pipettes, 5-100 & 500 ul
4. EIA Kit Microplate Washer
5. EIA Kit Microplate Reader at 450 nm



Mycobacterium Tuberculosis IgG ELISA (EIA) Kit Background Information
Mycobacterioses (tuberculosis, leprosy, atypical mycobacterioses, paratuberculosis, and perhaps Crohnas Disease) are the infectious diseases of men and animals with the largest diffusion on earth. The infectious agents of tuberculosis are acid-resistant rod-like formed bacteria of the family Mycobacteriaceae, genus Mycobacterium. The germ was detected by Robert Koch in 1882. Owing to the very high infectious power of pathogenic mycobacteria, early diagnosis is essential to prevent spreading of the disease. Convergence of various approaches is necessary to control the mycobacterioses, immune reactions and bacterial shedding being variable during the diseases. However, usual diagnostic procedures were up to now unsatisfactory and did not allow distinguishing among different mycobacterial species. The illness is normally transferred by droplets of saliva from infected persons. This ELISA test kit for the determination of IgG antibodies uses a cocktail of highly pure proteins in order to determine an immune response against the bacteria in human serum. A fresh or chronically active infection can be diagnosed by IgA and IgM tests, which are also available.


Mycobacterium Tuberculosis IgG ELISA (EIA) Test Principle
Mycobacterium tuberculosis antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Mycobacterium tuberculosis antigen takes place. After one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of IgG antibodies is directly proportional to the intensity of the color.


Mycobacterium Tuberculosis IgG ELISA Performance Characteristics
Intra-Assay Precision 7.6 %
Inter-Assay Precision 9.4 %
Inter-Lot-Precision 3.1 � 9.9 %
Analytical Sensitivity 1.09 U/ml
Recovery 86 � 95 %
Linearity 82 � 113 %
Cross-Reactivity: No cross-reactivity to Helicobacter pylori and Bordetella pertussis
Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/ml & tryglycerides up to 5.0 mg/ml


Precautions on Mycobacterium Tuberculosis IgG ELISA (EIA) Kit
All sera and plasma or buffers based upon, have been tested respective to HBsAg, HIV and HCV with recognized methods and were found negative. Nevertheless precautions like the use of latex gloves have to be taken.



Mycobacterium Tuberculosis IgG ELISA Related Kits:

Mycobacterium Tuberculosis IgA ELISA Kit

http://www.rapidtest.com/index.php?i=Mycobacterium-Tuberculosis-IgA-(TB)-ELISA-Test&id=99&cat=16

Mycobacterium Tuberculosis IgM ELISA Kit

http://www.rapidtest.com/index.php?i=Human-Mycobacterium-Tuberculosis-(TB)-IgM-ELISA-Test&id=101&cat=16


Diagnostic Automation Inc. also provides other Test Kits:

http://www.rapidtest.com/index.php?product=Rapid-Tests


For more information about ELISA Kits, Rapid Tests, CLIA tests, or IFA Tests, please contact our Customer Service Representatives at 818-591-3030.