The Diagnostic Automation SHBG ELISA test kit is an enzyme immunoassay for measurement of Sex-hormone-binding globulin (SHBG) in human serum and heparin plasma.





Material Provided with SHBG ELISA Kit:
1. Microplate: wells coated with anti-SHBG monoclonal antibody
2. Standard: STD0-STD4
3. Control
4. Assay Buffer
5. Enzyme Conjugate: Anti-SHBG antibody HRP-conjugate
6. Substrate Solution: TMB
7. Stop Solution: 0.5M H2SO4
8. Wash Solution 40X

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm





SHBG ELISA Kit Background Information:
SHBG is a Beta-globulin that specifically binds steroid hormones. The major site of SHBG synthesis is thought to be the hepatocytes. Its production is regulated by androgen/estrogen balance, thyroid hormones, insulin and dietary factors, among others. SHBG is involved in the transport of sex steroids in plasma. Its concentration is a major factor regulating their distribution between protein-bound and free states. Determination of SHBG concentration is mainly of importance in the evaluation of mild disorders of androgen metabolism and it allows identification of women with hirsutism who are likely to respond to estrogen therapy. Testosterone/ -ratios correlate well with both measured and calculated values for free testosterone, and help to discriminate between subjects with excessive androgen activity and normal individuals.





SHBG ELISA Test Principle:
SHBG ELISA kit is a solid phase ELISA based on the sandwich principle. The microtiter wells are coated with a monoclonal mouse antibody directed towards a unique antigenic site of the SHBG molecule. An aliquot of sample containing endogenous SHBG is incubated in the coated well. After a washing step, enzyme conjugate is added, which is a monoclonal anti-SHBG antibody conjugated with HRP. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration SHBG in the sample. For additional details please refer to the instructions for use.







Diagnostic Automation Inc. also provides other Steroid ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .