The Diagnostic Automation Toxoplasma gondii (Toxo) IgG ELISA test is intended for the detection and quantitative determination of IgG antibody to Toxoplasma gondii in human sera.




Materials Provided with Toxo IgG ELISA Kit:
1. Microassay plate:wells coated with inactivated Toxoplasma gondii antigen
2. Serum Diluent Type I, Ready for use
3. Calibrator: Human serum or defibrinated plasma
4. Positive Control: Human serum or defibrinated plasma
5. Negative Control: Human serum or defibrinated plasma
6. HRP Conjugate: Ready to use, Goat anti-human IgG
7. Chromogen/Substrate Solution Type I
8. Wash Buffer Type I, 20X
9. Stop Solution: Ready to use,1N H2SO4 solution

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450 single & 600-650nm dual wavelength




Toxo IgG ELISA Kit Background Information:
Toxoplasmosis is caused by the intracellular parasite Toxoplasma gondii and may be contracted by consuming contaminated meat or by contact with cat feces containing oocysts. In adolescence and adulthood, most infections are subclinical. However, if a pregnant woman contracts toxoplasmosis, it may be passed through the placenta to the fetus, resulting in congenital toxoplasmosis, which is a cause of mortality and malformation. Asymptomatic infants may develop anomalies later in life.



Toxo IgG ELISA Test Principle:
Purified Toxoplasma gondii antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Toxoplasma gondii IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibodyantigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.




For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.