Leishmania ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for
the qualitative screening of IgG antibodies to visceral Leishmania in serum.




Material Provided with Leishmania ELISA kit:
1.Test Strips Microwells: 96 test wells containing Leishmania antigens
2. Enzyme Conjugate: anti-human Ig Peroxidase-HRP
3. Positive Control: diluted Leishmania-positive human sera
4. Negative Control: diluted Leishmanianegative human sera
5. TMB Substrate Solution: TMB
6. Wash Concentrate 20X
7. Dilution Buffer
8. Stop Solution: 1 M phosphoric acid

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Leishmania ELISA kit Background Information:
Leishmania is a widespread disease affecting millions of people around the world. Globally, Leishmania is a serious infection that has spread over several continents, particularly Europe, India, Asia, Africa, and the Middle East. Transmission of Visceral Leishmaniasis, or VL, is induced by the bite of an infected sandfly, a parasitic member of the L. donovani complex. The sand fly is the host of Leishmania after it has already been in contact with an infected agent, such as a dog. Because visceral leishmaniasis attacks visceral organs such as the liver and spleen and the immune system, typically Leishmania has been closely associated with AIDS infections. Visceral Leishmania is a serious disease with high mortality rates.




Leishmania ELISA Test Principle:
The principle of the Leishmania ELISA test is a three-incubation process with the first incubation involving the coating of the wells with Leishmania antigen. Next, the wells must be washed to remove test sample and then Enzyme Conjugate is added. In the presence of this enzyme complex and peroxide, a substrate is added, and as a result of this combination, the substrate color will turn blue. The reaction is ended with the stop solution, and what appears is a yellow color instead of the blue.



Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Leishmania ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.