Posts Tagged ‘HCV ab ELISA Kit’

HCV ELISA kit | HCV-Ab Elisa kit, HCV-IgM ELISA kit

HCV ELISA KIT

 

Los Angeles based Diagnostic Automation/Cortez Diagnostics, Inc., offers a top quality HCV ELISA Kit that can immediately detect any hepatitis C infection.  This HCV ELISA Kit is used for screening blood donors and diagnosing patients infected with the hepatitis C virus.  Our company is committed to offering the best quality HCV ELISA Kit to our international customers.*  This convenient and easy-to-use HCV ELISA Kit is for in vitro diagnostic use only.

 

HCV ELISA kit

HCV ELISA kit

HCV ELISA KIT – DETECTION OF HEPATITIS C VIRUS

 

How do doctors find hepatitis C antibodies in the blood? To determine if hepatitis C antibodies are in the blood, doctors use a screening test called ELISA, which stands for Enzyme-Linked Immunosorbent Assay.  There are many different ELISA tests, so doctors must use one specifically for hepatitis C.  This is where the HCV ELISA Kit comes in.  The HCV ELISA Kit will search the blood sample for the hepatitis C antibody.  This HCV ELISA Kit is very sensitive and picks up approximately 95% of people infected with hepatitis C antibodies.  If the HCV ELISA Kit test finds any hepatitis C antibodies, that means an HCV infection is present.  The HCV ELISA Kit is tempered by its sensitivity.  For example, if the HCV ELISA Kit tests negative, patients are most likely free of hepatitis C infection.  However, if the HCV ELISA Kit test is positive, there is a small possibility that the result could be incorrect, resulting in a “false-positive.”  False-positives on this HCV ELISA Kit usually indicate more testing is required.

Since the introduction in 1990 of anti-HCV screening of blood donations, like this HCV ELLISA Kit, the incidence of this infection in transfusion recipients has been significantly reduced. The first generation of HCV ELISA Kit tests showed limited sensitivity and specificity and was produced using recombinant proteins complementary to the NS4 (c100-3) region of the HCV genome as antigens.  Second generation HCV ELISA Kit tests, which included recombinant / synthetic antigens from the Core (c22) and nonstructural regions NS3 (c33c, c100-3) and NS4 (c100-3, c200), resulted in a remarked improvement in sensitivity and specificity. Clinical studies show that a significant amount of HCV infected individuals develop antibodies to NS5 non-structural protein of the virus.  For this, the third generation HCV ELISA Kit tests include antigens from the NS5 region of the viral genome in addition to NS3 (c200), NS4 (c200) and the Core (c22).  Third generation HCV ELISA KIT tests have improved sensitivity and shorten the time between infection with HCV and the appearance of detectable antibodies (window period) to 60 days.

 

HCV ELISA KIT – TWO KINDS OF TESTS

This HCV ELISA Kit is available in two unique formats.   One easy-to-use HCV ELISA Kit, also known as HCV ab ELISA Kit, is an enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies to hepatitis C virus in human serum or plasma. This particular HCV ELISA Kit is intended for screening blood donors and diagnosing patients related to infection with hepatitis C virus.  This particular HCV ELISA Kit employes solid phase, indirect ELISA assay for detection of antibodies to HCV in a two-step incubation procedure. This HCV ELISA Kit is intended for in vitro use only.  This HCV ELISA kit is intended ONLY for testing of individual serum or plasma samples. Do not use it for testing of cadaver samples, saliva, urine or other body fluids, or pooled (mixed) blood.

 

The other HCV ELISA Kit, also known as HCV IgM EIA kit,  is an enzyme-linked immunosorbent assay for qualitative detection of IgM-class antibodies to hepatitis C virus in human serum or plasma. This HCV ELISA Kit is intended for clinical diagnosis, management and follow-up of patients related to infection with hepatitis C virus.  The HCV ELISA Kit is a two-step incubation, indirect principle procedure.  This HCV ELISA Kit for IgM-class antibodies is intended for in vitro use only.

 

 

*not available in Europe and U.S.

 

 

OTHER EFFECTIVE HEPATITIS TEST KITS

 

Diagnostic Automation/Cortez Diagnostics, Inc. also offers the best quality HCV Rapid Tests to detect the Hepatitis C virus.  This One Step Strip Style Anti-HCV Test (Hepatitis C Rapid Test) is a rapid direct binding test for the visual detection of hepatitis C antibodies (anti-HCV).  This Hepatitis C Rapid Test is used as an aid in the diagnosis of hepatitis C infection.   The One Step Hepatitis C Rapid Test is based on the principle of double antigen sandwich immunoassay for determination of anti-HCV in serum.  Purified recombinant antigens are employed to identify anti-HCV specifically.  This One Step Hepatitis C test is very sensitive and only takes 10-20 minutes for the result to be read.  Test results are read visually without any instrument.

Customers who use the HCV ELISA KIT and HCV Rapid Test also inquire about our Hepatitis B ELISA Test Kit and Hepatitis B Rapid Test that detect the Hepatitis B virus in human serum or plasma.  These widely-used Hepatitis ELISA Test Kits and Hepatitis Rapid Tests are the most convenient and inexpensive Hepatitis ELISA Test Kits and Hepatitis Rapid Tests on the market today.  For more information about the various Hepatitis ELISA Test Kits or Hepatitis Rapid Test Kits, please contact our Customer Service department.

 

Also available at Diagnostic Automation/Cortez Diagnostics, Inc. are HAV ELISA Test Kits, HBV ELISA Test Kits, HDV ELISA Test Kits, HEV ELISA Test Kits, HGV ELISA Test Kits, and HTLV ELISA Test Kits.

 

RELATED PRODUCTS

 

Diagnostic Automation/Cortez Diagnostics, Inc. is a USA based company manufacturing and distributing ELISA Test Kits (Autoimmune diseases ELISA Kit, Cardiac Markers, Allergy ELISA kits, HIV ELISA kits, etc.) and Rapid Tests (HIV Rapid Test, Drug Tests or Drugs of Abuse Rapid Tests, Cardiac Rapid Tests, Allergy Rapid Tests, Cancer Rapid Tests, Pregnancy Rapid Tests, Urine Reagent Strips, etc.) and IFA Kits (Autoimmune diseases IFA Kits, Autoimmune Disease Kits, Bacterial Disease IFA Kits, etc.) and Chemilluminescence immuno assays (Infectious Disease kits, Fertility Assays/CLIA kits, Diabetes Assays/CLIA kits, Cardio-Vascular/CLIA kits, Tumor Marker Assays kits, etc.) and ELISA readers – ELISA washers and Serology test kits.

 

Please contact our Customer Service Department for information about this unique HCV ELISA Kit, or ELISA Test Kits, CLIA Kits, IFA Kits, or any other drug testing products of interest.

 

HCV ELISA kit – HCV ab ELISA Kit – HCV igM ELISA Kit

USING HEPATITIS ELISA TEST KITS

(HCV ELISA KITS) to detect HEPATITIS C VIRUS

 

Los Angeles based Diagnostic Automation/Cortez Diagnostics, Inc., a leading provider of Hepatitis ELISA Test Kits, offers top quality HCV ELISA kits at competitive prices and in a timely manner.  Our company is committed to offering the best quality HCV Elisa kits worldwide.  These convenient and easy-to-use HCV Elisa Kits (Hepatitis C ELISA Test Kits) can immediately detect any Hepatitis C infection.

 

Hepatitis C virus (HCV) is an envelope, single stranded positive sense RNA (9.5 kb) virus belonging to the family of Flaviviridae. Six major genotypes and series of subtypes of HCV have been identified. Isolated in 1989, HCV is now recognized as the major cause for transfusion associated non-A, non-B hepatitis. The disease is characterized with acute and chronic form although more than 50% of the infected individuals develop severe, life threatening chronic hepatitis with liver cirrhosis and hepatocellular carcinomas. Since the introduction in 1990 of anti-HCV screening of blood donations, the incidence of this hepatitis C infection in transfusion recipients has been significantly reduced.

 

There are two kinds of HCV ELISA kits available through Diagnostic Automation/Cortez Diagnostics, Inc.  One easy-to-use HCV ELISA Kit, also known as HCV ab ELISA Kit, is an enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies to hepatitis C virus in human serum or plasma. It is intended for screening blood donors and diagnosing patients related to infection with hepatitis C virus.  The other convenient HCV ELISA Kit is an enzyme-linked immunosorbent assay for qualitative detection of IgM-class antibodies to hepatitis C virus in human serum or plasma. It is intended for clinical diagnosis, management and follow-up of patients related to infection with hepatitis C virus.

 

RESULTS OF HCV ELISA TESTS

 

 

HCV ELISA kit (HCV ab ELISA Kit)

This anti-HCV employs solid phase, indirect ELISA method for detection of antibodies to HCV in two-step incubation procedure. Polystyrene microwell strips are pre-coated with recombinant, highly immunoreactive antigens corresponding to the core and the non-structural regions of HCV (third generation HCV ELISA). During the first incubation step, anti-HCV specific antibodies, if present, will be bound to the solid phase pre-coated HCV antigens. The wells are washed to remove unbound serum proteins, and rabbit anti-human IgG antibodies (anti-IgG) conjugated to the enzyme horseradish peroxidase (HRP-Conjugate) is added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody(IgG) complexes previously formed and the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the antigen-antibody-anti-IgG (HRP) immunocomplex, the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and it is proportional to the amount of antibody captured in the wells, and to the amount of antibody in the sample respectively. Wells containing samples negative for anti-HCV remain colorless.

 

HCV ELISA Kit (HCV IgM ELISA Kit)

This kit employs indirect ELISA assay for detection of IgM antibodies to HCV in two-step incubation procedure. Polystyrene microwell strips are pre-coated with recombinant, highly immunoreactive antigens corresponding to the core and non-structural regions of HCV. During the first incubation step, HCV-IgM specific antibodies, if present, will be bound to the solid phase pre-coated HCV antigens. The wells are washed to remove unbound materials, and then, rabbit anti-human IgM antibodies (anti-IgM) conjugated to horseradish peroxidase (HRP-Conjugate) are added. During the second incubation step, these labeled antibodies will be bound to any antigen-IgM complexes previously formed and the unbound HRP-Conjugat is removed by washing.  Chromogen solutions  containing tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the antigen- antibody-anti-IgM (HRP) immunocomplex; the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for HCV-IgM remain colorless.

 

EFFECTIVE HEPATITIS TEST KITS

 

Diagnostic Automation/Cortez Diagnostics, Inc. also offers the best quality HCV Rapid Tests to detect the Hepatitis C virus.  This One Step Strip Style Anti-HCV Test (Hepatitis C Rapid Test) is a rapid direct binding test for the visual detection of hepatitis C antibodies (anti-HCV).  This Hepatitis C Rapid Test is used as an aid in the diagnosis of hepatitis C infection.   The One Step Hepatitis C Rapid Test is based on the principle of double antigen sandwich immunoassay for determination of anti-HCV in serum.  Purified recombinant antigens are employed to identify anti-HCV specifically.  This One Step Hepatitis C test is very sensitive and only takes 10-20 minutes for the result to be read.  Test results are read visually without any instrument.

Customers who use the HCV ELISA KITS and HCV Rapid Tests also inquire about our Hepatitis B ELISA Test Kits and Hepatitis B Rapid Tests that test for the Hepatitis B virus in human serum or plasma.  These widely-used Hepatitis ELISA Test Kits and Hepatitis Rapid Tests are the most convenient and inexpensive Hepatitis ELISA Test Kits and Hepatitis Rapid Tests on the market today.  For more information about the various Hepatitis ELISA Test Kits or Hepatitis Rapid Test Kits, please contact our Customer Service department.

 

Also available at Diagnostic Automation/Cortez Diagnostics, Inc. are HAV ELISA Test Kits, HBV ELISA Test Kits, HDV ELISA Test Kits, HEV ELISA Test Kits, HGV ELISA Test Kits, and HTLV ELISA Test Kits.

 

RELATED PRODUCTS

 

Diagnostic Automation/Cortez Diagnostics, Inc. is a USA based company manufacturing and distributing ELISA Test Kits (Autoimmune diseases ELISA Kit, Cardiac Markers, Allergy ELISA kits, HIV ELISA kits, etc.) and Rapid Tests (HIV Rapid Test, Drug Tests or Drugs of Abuse Rapid Tests, Cardiac Rapid Tests, Allergy Rapid Tests, Cancer Rapid Tests, Pregnancy Rapid Tests, Urine Reagent Strips, etc.) and IFA Kits (Autoimmune diseases IFA Kits, Autoimmune Disease Kits, Bacterial Disease IFA Kits, etc.) and Chemilluminescence immuno assays (Infectious Disease kits, Fertility Assays/CLIA kits, Diabetes Assays/CLIA kits, Cardio-Vascular/CLIA kits, Tumor Marker Assays kits, etc.) and ELISA readers – ELISA washers and Serology test kits.

 

Please contact our Customer Service Department for information about any of these ELISA Test Kits, CLIA Kits, IFA Kits, or any other drug testing products of interest.