Total Human IgE ELISA kit description:

The Total Human IgE ELISA Kit is for quantitative measurement of total human Immunoglobulin E (IgE) specific for common allergens in human serum.




Material Provided with Total Human IgE ELISA Kit:
1.Antibody-coated microtiter wells, 96 wells plate
2.Reference standards, 0, 10, 50, 100, 400, 800 IU/ml
3.Total Human IgE EIA Kit Zero Buffer
4.Enzyme Conjugate Reagent
5.Total Human IgE Elisa kit TMB Substrate
6.Stop Solution
7.Total Human IgE Elisa Test Wash Buffer (50X)

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm filter




Total Human IgE ELISA Kit Background Information:
Patients with atopic allergic diseases such as atopic asthma, atopic dermatitis, and hay fever have been shown to exhibit increased total immunoglobulin E (IgE) levels in blood. In general, elevated levels of IgE indicate an increased probability of an IgE-mediated hypersensitivity, responsible for allergic reactions. Parasitic infestations such as hookworm, and certain clinical disorders including aspergillosis, have also been demonstrated to cause high levels of IgE. Decreased levels of IgE are found in cases of hypogammaglobulinemia, autoimmune diseases, ulcerative colitis, hepatitis, cancer, and malaria. Cord blood or serum IgE levels may have prognostic value in assessing the risk of future allergic conditions in children. Certain groups of white blood cells, including basophils and tissue mast cells, have membrane receptors for the IgE molecule. These target cells, through a series of complex reactions, form a combination of a specific allergen with antibody-sensitized basophils such as histamine, into the blood stream. As a result of these biochemical mediators, there is a constriction of smooth muscles, dilation of small blood vessels, activation of blood platelets, and irritation of skin nerve endings characteristic of allergic reactions.Typical clinical symptoms of immediate hypersensitivity are inflammation and itching in a skin reaction, or congestion in a bronchial reaction. The IgE serum concentration in a patient is dependent on both the extent of the allergic reaction and the number of different allergens to which patient is sensitized. Nonallergic normal individuals have IgE concentrations that vary widely and increase steadily during childhood, reaching their highest levels at age 15 to 20, and thereafter remaining constant until about age 60, when they slowly decline.

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Total Human IgE ELISA Test Principle:
This Total Human IgE ELISA kit is an IgE quantitative Test Kit based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-IgE antibody for solid phase (microtiter wells) immobilization and another anti-IgE antibody in the antibody-enzyme
horseradish peroxidase conjugate solution. For additional details please refer to the instructions for use.




Notable features of the Total Human ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Allergy ELISA kits follow a similar method. See the Total Human IgE ELISA Test Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation also provides other Human Allergen Specific ELISA kits: Human IgA ELISA Test kit, Human Allergen Specific IgM ELISA Test kit, and Human Allergen Specific IgG ELISA. Additionally, Total Human ELISA Kits are available: Total Human IgA ELISA Test kit, Total Human IgM ELISA Test kit, Total Human IgG ELISA Test kit, and Total Human IgE ELISA Test kit. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .