HBeAg ELISA kit description:

"Export Use Only"
This HBeAg ELISA kit is for the qualitative detection of hepatitis B viruse antigen (HBeAg) in human serum or plasma. It is for diagnosis and monitoring of patients related to infection with the hepatitis B virus.




Materials included with HBeAg ELISA test kit:
1. Microwell Plate: 96 wells coated with monoclonal antibodies reactive to HBeAg
2. Negative Control: Protein-stabilized buffer non-reactive for HBeAg
3. Positive Control: Recombinant, non-infective HBeAg diluted in protein-stabilized buffer
4. HRP-Conjugated Reagent: HRP-conjugated anti-HBe antibodies
5. Stock Wash Buffer: PH 7.4, 20x PBS
6. Chromogen Solution A: Urea peroxide solution
7. Chromogen Solution B: TMB solution
8. Stop Solution: Diluted sulfuric acid solution
9. Plastic Sealable Bag
10. Cardboard Plate Cover
11. Package Insert

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate washer
5. EIA kit Microplate Reader with 450nm or dual wavelength 450 & 630nm




HBeAg ELISA test Background Information:
The Hepatitis B, e-antigen, is a virus protein that is closely associated with the hepatitis B virus
replication, indicating a high degree of infectivity. HBeAg normally appears shortly after HBsAg, and is detectable from a few days to several weeks later. During treatment and recovery, the titer of HBeAg declines and is replaced by the corresponding antibody (anti-HBe). In chronic hepatitis B infections, elevated levels of HBeAg can be detected for years, which is a sign for a heavy quantity of the virus. In some chronic HBsAg positive patients, HBeAg is undetectable due to HBV mutations suggesting low levels of viral replication. The anti-HBeAg antibody appearance in the blood is recognized to be a clinical sign of recovery from the infection, respectively, if HBeAg is considered the specific marker of infectivity in that patient.




HBeAg ELISA test Principle:
This Hepatitis B kit has a sandwich ELISA method in which, polystyrene microwell strips are precoated with monoclonal antibodies specific to HBeAg. Human serum or plasma specimen is added to the microwell together with a second monoclonal antibody conjugated to horseradish peroxidase (HRPConjugate). The specific immunocomplex formed in case of presence of HBeAg in the sample is captured on the solid phase, during incubation.


Most Infectious Disease ELISA kits follow a similar method. See the HCV Ab ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

*Not to be sold, shipped, or distributed in The United States.