PAPP-A ELISA kit description:

The Diagnostic Automation PAPP-A ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Pregnancy associated plasma protein A (PAPP-A) in human serum and plasma.




Material Provided with PAPP-A ELISA Kit:
1. Microtiterwells: wells coated with anti-PAPP-A antibody (polyclonal)
2. Standards: Concentrations: 0; 1; 2.5; 5.0; 15.0; 30.0 ug/mL
3. Controls: (low and high)
4. Assay Buffer
5. Enzyme Conjugate 11X concentrate
6. Conjugate Diluent
7. Substrate Solution
8. Stop Solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




PAPP-A ELISA Kit Background Information:
PAPP-A is a protein produced by the developing placenta Its concentration in the maternal blood increases rapidly after the 7th week of pregnancy. The measurement of PAPP-A in the first trimester of pregnancy has been reported as a useful marker in antenatal screening for Down Syndrome and other fetal aneuploidies. Reduced PAPP-A values in combination with maternal age, the measurement of free Beta-HCG and the ultrasonic determination of nuchal translucency in pregnancy weeks 11 to 14 may detect up to 90 % of pregnancies with Down syndrome. Diagnostic Automation PAPP-A ELISA EIA-2397 may be used for the risk assessment of Down's syndrome (trisomy 21) in the first trimester of pregnancy.




PAPP-A ELISA Test Principle:
This PAPP-A ELISA Kit is a solid phase ELISA based on the sandwich principle. The microtiter wells are coated with a polyclonal anti PAPP-A antibody. An aliquot of patient sample containing endogenous PAPP-A is incubated in the coated well with assay buffer. After incubation the unbound material is washed off. In the second incubation step a sandwich complex is formed with a polyclonal anti PAPP-A antibody peroxidase conjugate. Having added the substrate solution, the intensity of color developed is proportional to the concentration of PAPP-A in the patient sample. For additional details please refer to the instructions for use.





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