Prolactin (PRL) ELISA kit description:

The Diagnostic Automation PRL EIA test kit is for the quantitative determination of human Prolactin (PRL) concentration in human serum.




Material Provided with PRL ELISA Kit:
1. Prolactin EIA Kit antibody coated microtiter wells
2. Reference standard 2 sets
3. Enzyme Conjugate Reagent
4. Prolactin Elisa Test TMB Substrate
5. Stop Solution, 12 ml
6. Prolactin Elisa Kit Wash Buffer Concentrate 50X

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm





Prolactin ELISA Kit Background Information:
Human prolactin is secreted from the anterior pituitary gland in both men and woman. During pregnancy, prolactin levels increase progressively to between 10 and 20 times normal values, declining to non-pregnant levels by 3-4 weeks post-partum. Breast-feeding mothers maintain high levels of prolactin, and it may take several months for serum concentrations to return to non-pregnant levels. The determination of prolactin concentration is helpful in diagnosing hypothalamic-pituitary disorders. Microadenomas may cause hyperprolactinemia, sometimes associated with male impotence. High prolactin levels are commonly associated with galactorrhea and amenorrhea. Prolactin concentrations have been shown to be increased by estrogens, TRH, and several drugs affecting dopaminergic mechanism. Prolactin levels are elevated in renal disease and hypothyroidism, and in some situations of stress, exercise, and hypoglycemia. Mildly elevated prolactin concentrations should be evaluated taking these considerations into account. Prolactin concentrations may also be increased by drugs such as chloropromazine and reserpine, and may be lowered by bromocyptine and L-dopa.





Prolactin ELISA Test Principle:
The Prolactin quantitative test kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-prolactin antibody for micro titer wells immobilization and another mouse monoclonal anti-prolactin antibody in the antibody- enzyme horseradish peroxidase conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in the prolactin molecules being sandwiched between the solid phase and enzyme-linked antibodies. For additional details please refer to the instructions for use.





Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .