Chloramphenicol ELISA kit description:

Diagnostic Automation Inc. offers a Chloramphenicol Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative determination of Chloramphenicol in Food. This Chloramphenicol Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect Chloramphenicol in food.




Materials Provided with Chloramphenicol Elisa Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with an antibody binding protein
2. Chloramphenicol Standards (0; 0.05; 0.1; 0.5; 1; 5 ng/mL): 6 vials with 1 mL each
3. Anti-Chloramphenicol Antibody (sheep): 6 mL
4. Conjugate (Chloramphenicol-Peroxidase): 6 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample diluent (PBS) 2x
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




Chloramphenicol ELISAKit Background Information:
Due to its outstanding antibacterial properties, chloramphenicol is an often used antibiotic in the production of milk, meat and eggs. In humans it leads to haematotoxic side effects like the chloramphenicol induced aplastic anemia. This has caused low limits in Germany, e.g. 1ug/kg for milk, meat and eggs. Until now, the chloramphenicol concentration was determined by radioimmunoassay or by gas chromatography. However, compared with conventional methods, enzyme immunoassays show some essential advantages. There is no need to work with radioactive material; the required assay time on this Chloramphenicol Food ELISA Test Kit is shorter and the sensitivity is better than with chromatographic methods. Forty samples on this Food ELISA test kit can be assayed in duplicate within 60 minutes.




Chloramphenicol Food ELISA Test Principle:
The Chloramphenicol Food ELISA Kit is a quantitative Chloramphenicol food test based on the principle of the enzyme-linked immunosorbent assay. An antibody binding protein is coated on the surface of a microtiter plate. Chloramphenicol containing samples or standards and an antibody directed against chloramphenicol are given into the wells of the microtiter plate. The chloramphenicol contained in samples or standards will bind to the antibody which reacts with the binding protein coated onto the microtiter plate




Diagnostic Automation Inc. offers several popular Food ELISA Test Kits: Fumonisin ELISA Kit, Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.