Histamine ELISA kit description:

Diagnostic Automation Inc. offers a Histamine Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative determination of Histamine in Food. This Histamine Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect histamine in food.




Materials Provided with Histamine Food ELISA Kit:
1. Microtiter plate: wells coated with histamine conjugate
2. Histamine Standards (0; 2; 4; 10; 40; 100 ng/mL): 6 vials
3. Reaction Solution
4. Neutralizing Solution
5. Anti-Histamine Antibody (mouse)
6. Conjugate (anti-mouse-IgG-HRP)
7. Substrate Solution (TMB)
8. Stop Solution (0.5 M H2SO4)
9. Sample Diluent (PBS)
10. Washing Solution (PBS + Tween 20) 10x
11. Two plastic foils
12. Plastic bag to store unused microtiter strips
13. Instruction Manual

Materials required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




Histamine Food Elisa Kit Background Information:
Histamine is a biogenic amine, which is formed by enzymatical decarboxylation from the amino acid histidine. It occurs in the mast cells and basophilic white cells bound to heparin. In the course of a type I allergy, endogenic histamine from the mast cells and basophilic leucocytes is released after antigen binding to membrane-associated IgE, and typical allergical reactions appear. But histamine can also enter the human body via the nutrition and can thus cause pseudoallergic food intolerances. The appearing symptoms are not distinguishable from those of a real allergy. The term, food allergy is however only used, if the pathologic symptoms develop in the course of an immunological reaction. Non-immunological reactions to food are defined as food intolerances. Food intolerances, which are caused by increased histamine concentrations, are clinically characterized by rash, diarrhea, vomiting, nausea, itching, headache and asthma. The extent of the reaction is dependent on the ingested amount of histamine. These reactions can be enhanced by a lack of diaminoxidase. Separated from histamine intolerances toxic reactions exist, which are caused by very high histamine concentrations. Toxic histamine concentrations, which can cause the so-called scombroid reaction, appear after bacterial degradation of protein-rich food, especially fish. Histamine is found above all in cheese, fish, spinach, smoked products and red wine. After an appropriate sample preparation on this Histamine Food ELISA Test Kit, 40 samples can be tested in duplicate within 80 minutes.




Histamine Food Elisa Test Principle:
The Histamine Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. A histamine conjugate is bound on the surface of a microtiter plate. Samples or standards containing derivatized histamine and an antibody directed against histamine are given into the wells of the microtiter plate on the Histamine ELISA Kit. Immobilized and free histamine compete for the antibody binding sites.



Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.