E.coli Antigen In Food ELISA Kit description:

The Diagnostic Automation, INC. E. coli O157 assay is an enzyme-linked immunosorbent assay (ELISA) to screen food products for the presence of E. coli O157 antigen. E. coli O157 ELISA is a rapid and reliable test which significantly reduces the time required to screen foods for the presence of E. coli O157.



Materials Provided with E. coli O157 Elisa Kit:
1. Microwell test strips containing anti- E. coli O157 polyclonal antibodies
2.Test strip holder
3. Enzyme Conjugate: containing peroxidase conjugated anti- E. coli O157 polyclonal antibody
4. Positive control: containing 2 ml of killed E. coli O157 antigen in a buffered base
5. Negative control:containing 2 ml of buffered base
6. Chromogen: containing 11 ml of chromogen tetramethylbenzidine (TMB)
7. Wash Concentrate 20X: containing concentrated buffer and surfactant
8. Stop solution: containing 1 M phosphoric acid

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




E. coli O157 Elisa Kit Background Information:
Strains of Escherichia coli that produce Shiga-like toxins (SLTs), also known as Verocytotoxins (VTs), are an important cause of human disease. Clinical manifestations of infection include diarrhea, hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Although Shiga-like toxin producing E.coli (SLTEC) belong to many O serogroups, serotypes O157:H7 and O157:H are the predominant SLTEC associated with HC and HUS. Outbreaks of HC and HUS due to E. coli O157 have been linked to consumption of undercooked ground beef. Prevalence studies indicate that up to 3.7% of retail meats may contain E. coli O157. Although this may seem low compared to some other foodborne pathogens, the seriousness of disease caused by E. coli O157 has made this pathogen a major food safety concern. Detection of E. coli O157:H7 in meats by cultural methods is time consuming, requiring several days to obtain presumptive positive results. Typically, meat samples are cultured in primary enrichment broth which is plated onto sorbitol-MacConkey (SMAC) agar. Non-sorbitol fermenting colonies are then subcultured for identification by serotyping or related methods.



E. coli O157 Elisa Test Principle:
The Diagnostic Automation, INC. E. coli O157 ELISA is a double antibody (sandwich) ELISA utilizing specific anti-E. coli O157 antibodies coated to microwells. Primary enrichment cultures grown for 8-16 hours can be tested in less than one hour, allowing ELISA-negative product to be released within 24 hours. Enrichment broths presumptively positive for E. coli O157 on the basis of positive ELISA tests can be cultured further for confirmation by standard methods.




For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.