T3 ELISA kit description:

The T3 ELISA is intended for the quantitative determination of the Free Triiodothyronine (T3) concentration in human serum.




Material Provided with T4 ELISA Kit:
1. Microplate: Anti-T3 Antibody-Coated Wells
2. T3 HRPO Conjugate Concentrate
3. T3 HRPO Conjugate Diluent
4. Reference Standard
5. TMB Substrate
6. Stop Solution
7. Wash Buffer Concentrate 50X

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm





T3 ELISA Kit Background Information:
The hormones thyroxine (T4) and 3,5,3- thiiodothyronine (T3) circulate in the bloodstream, mostly bound to the plasma protein, thyroxine binding globulin (TBG). The concentration of T3 is much less than that of T-4, but its metabolic potency is much greater. T3 determinations an important factor in the diagnosis of thyroid disease. Its measurement has uncovered a variant of hyperthyroidism in thyrotoxic patients with elevated T3 values and normal T4 values. An increase in T3 without an increase in T4 is frequently a forerunner of recurrent thyrotoxicosis in previously treated patients. The clinical significance of T3 is also evident in patients in whom euthyroidism is attributable only to normal T3, although their 4 values are subnormal. T3 determination is also useful in monitoring both patients under treatment for hyperthroidism and patients who have discontinued anti-thyroid drug therapy. In addition to hyperthyroidism, T3 levels are elevated in women who are pregnant, and in women receiving oral contraceptives or estrogen treatment, paralleling TBG increases in a manner analogous to T4 levels. Likewise, a reduction in TBG concentration decreases T3 concentration. These changes in the T3 level, however, are not a true reflection of thyroid status.




T3 ELISA Test Principle:
The T3 EIA test is based on the principle of a solid phase enzyme-linked immunoassay. A measured amount of patient serum, and a constant amount of T3 conjugated with horseradish peroxidase are added to the microtiter wells coated with a certain amount of anti-T3 antibody. During incubation, the anti-T3 antibody is bound to the second antibody on the wells, and T3 and conjugated T3 compete for the limited binding sites on the anti-T3 antibody. For additional details please refer to the instructions for use.





Diagnostic Automation Inc. also provides other Thyroid ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .