HAV IgG ELISA kit description:

"Export Use Only"
The HAV IgG ELISA test is an enzyme-linked immunosorbent assay (ELISA) which is used for the qualitative determination of IgG-class antibodies to hepatitis A virus (HAV-IgG) in serum/plasma. The purpose of the HAV IgG ELISA Test is for diagnosing and monitoring of patients suspected of hepatitis A virus.


Materials Provided with HAV IgG ELISA Kit:
1. Microwell strips: purified HAV antigens coated wells
2. Negative Control: Protein-stabilized buffer tested non-reactive for HAV-IgG
3. Positive Control:HAV-IgG antibodies diluted
4. HRP Conjugate: HRP-HAV-IgG antibodies
5. Stock Wash Buffer 20x
6. Chromogen Solution A: Urea peroxide solution.
7. Chromogen Solution B: TMB
8. Stop Solution
9. Plastic Sealable Bag
10. Cardboard Plate Cover
11. Package Inserts

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength



HAV IgG ELISA Test Background Information:
Infections usually onset in early childhood, HAV can adversely affect the liver, but acute complications or chronic illness do not necessarily occur because Hepatitis A is a self-limited disease. Causes of the infection are connected to unsanitary conditions and poor hygiene within densely populated areas. Thus, the fecal-oral route of the disease is easily transmitted in this type of setting. One single infected person could cause an entire outbreak. Hepatitis A is a non-enveloped positive strand RNA virus with a linear single strand genome, encoded for only one known serotype. Within HAV are four major, structural polypeptides, concentrated exclusively in the cytoplasm of human hepatocites. An HAV infection causes a strong immunological response, and within a few days after the onset of symptoms, elevated levels of IgM, and subsequently IgG, are identified. Past infection and immunity to HAV are indicated by the presence of IgG. Thus, the phase, classification, and source of the infection can be confirmed when the serological identification of HAV IgG is known.



HAV IgG ELISA Test Principle:
The principle method of the HAV IgG ELISA is a solid phase assay that features a one-step incubation competitive principle. When HAV IgG antibodies are present, they compete with monoclonal HAV IgG antibodies designated with HRP-Conjugate for a fixed amount of purified HAV antigens that were pre-coated in the wells. If HAV IgG is not present, HAV IgG (HRP labeled) will be bound together with antigens inside the wells.


For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

* Not to be sold, shipped or distributed in The United States.