HBcAb IgM ELISA kit description:

"Export Use Only"
The HBcAb IgM ELISA is an enzyme-linked immunosorbent assay for the qualitative identification of IgM class antibodies to hepatitis B core antigen in human serum/plasma. The HBcAb IgM ELISA test result is an aid for clinical lab diagnosis of hepatitis B infected patients.




Materials included in HBcAb IgM ELISA test kit:
1. Microwell Plate: 96 wells coated with anti-IgM antibodies
2. Negative Control: Protein-stabilized buffer non-reactive for anti-HBc IgM
3. Positive Control: anti-HBc IgM antibodies diluted in protein-stabilized buffer
4. HRP-Conjugated Reagent: HRP-conjugated, HBcAg, labeled with monoclonal anti- HBc
5. Stock Wash Buffer: PH 7.4, 20x PBS
6. Chromogen Solution A: Urea peroxide solution
7. Chromogen Solution B: TMB solution
8. Stop Solution: 2.0M H2SO4
9. Plastic Sealable Bag
10. Cardboard Plate Cover
11. Package Insert

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate washer
5. EIA kit Microplate Reader with 450nm or dual wavelength 450 & 630nm




HBcAb IgM ELISA test Background Information :
As part of the Hepadnaviridae family, HBV is an enveloped, double-stranded DNA virus that is a primary cause of hepatitis transmission through blood. In order to classify hepatitis B infection, the serological markers need to be identified during the three phases of the infection - incubation, acute, and convalescent. The main component of the virus is Hepatitis B core antigen (HBcAg). This core antigen is comprised of a single polypeptide of approximately 17kD that is discharged upon disaggregation of the core particles. At least one immunological determinant is present in the antigen. Shortly after the onset of HBsAg, antibodies to HBcAg (anti-HBc total antibody and IgM) appear and are always present. In isolated cases, a Hepatitis B infection can be contracted without immunologically detectable anti-HBc. This is found usually in immunosuppressed patients. During the acute stages of hepatitis infection, particles of anti-HBc IgM synthesis are detectable. This presence confirms reactivation of HBV in hepatocites and causes permanent IgM low titers. The presence of IgM and total anti-HBc signifies a recent or ongoing HBV infection. A laboratory diagnosis, or a confirmation of a negative HBV infection, can be reached when this test is performed concurrently with tests for other HBV serological markers.




HBcAb IgM ELISA test Principle:
The principle of the HBcAb IgM ELISA is based on a two-step incubation, solid phase antibody capture ELISA. Polystyrene microwell strips are pre-coated with antibodies intended for human immunoglobulin M proteins (anti-u chain).



Most Infectious disease ELISA kits follow a similar method. See the HCV Ab ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.


For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

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