HSV-1 IgM ELISA kit
| Name |
HSV-1 IgM ELISA Test |
|---|---|
| Full name |
Human Herpes Simplex 1 IgM (HSV-1 IgM) ELISA Test kit |
| Category Name | Infectious Disease ELISA kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | ELISA principle- Indirect; Antigen Coated Plate |
| Detection Range | Qualitative elisa assay- Positive, Negative and Cut-off |
| Sample | 5uL Serum |
| Specificity | 96% |
| Sensitivity | 75% |
| Total Time | ~75 min |
| Shelf Life | 12 Months from the manufacturing date |
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HSV-1 IgM ELISA kit description:
The Diagnostic Automation HSV1 IgM is intended for the detection of IgM antibodies to herpes simplex virus (HSV) 1.
Materials Provided with HSV1 IgM ELISA kit:
1. Microwell Plate coated with purified HSV1 antigen
2. Absorbent Solution
3. Calibrator
4. Negative Control
5. Positive Control
6. Washing Concentrate 20X
7. Enzyme Conjugate
8. TMB
9. Stop Solution
Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. Microshaker
4. ELISA kit Microplate washer
5. ELISA kit Microplate Reader with 450nm or dual 450 & 630nm wavelength
HSV1 IgM ELISA Kit Background Information:
Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. Diagnostic Automation HSV1 IgG Elisa test kit is an accurate serologic method to detect HSV specific antibody in serum sample.
HSV1 IgM ELISA Test Principle:
Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the HSV 1 IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate
catalytic reaction is stopped at a specific time. For additional details please refer to the instructions for use.
For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030 818-591-3030.