Chlamydia Trachomatis IgA ELISA Kit description:

The Diagnostic Automation Chlamydia Trachomatis IgA ELISA test kit is intended for the determination of specific IgA antibody to Chlamydia in a single human serum sample, by an Enzyme-Linked Immunosorbent Assay.



Materials Provided with the Chlamydia Trachomatis IgA ELISA kit:
1. Microwell strips: wells coated with Chlamydia Trachomatis antigen
2. Sample Diluent: Blue Color Solution
3. Negative Control
4. Calibrator
5. Positive Control
6. Washing concentrate 20x
7. Enzyme conjugate
8. Chromogenic Substrate
9. Stop solution: 2 N HCl.


Materials & Instrumentations Required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Dry incubator or water bath
4. Microshaker for mixing
5. EIA kit Microplate Washer
6. EIA kit Microplate Reader at 450nm wavelength



Chlamydia Trachomatis IgA ELISA Test Background Information:
Chlamydia Trachomatis is one of the most common human pathogens. Of the 15 recognized serotypes, 4 (A, B, Ba, and C) have been shown to cause hyperendemic blinding trachoma, a disease which afflicts hundreds of millions of people in developing countries. Three serotypes (L-1, L-2, and L-3) are the causes of lymphogranuloma venereum (LGV), a sexually transmitted systemic disease. The other serotypes (D through K) have been associated with genital tract infections and sporadic cases of conjunctivitis in industrialized societies. These agents are the major recognized cause of nongonococcal urethritis in men, in whom they may also cause epididymitis. In women, C. trachomatis causes cervicitis and has been associated with acute salpingitis. Infants born through an infected birth canal may contract the infection and then develop inclusion conjunctivitis of the newborn and/or the characteristic chlamydial pneumonia syndrome. High levels of anti-Chlamydia IgG antibody are of diagnostic value in chronic or systemic infections such as salpingitis, mechanical infertility, perihepatitis, epididymitis, Reiter syndrome and pneumonitis.



Chlamydia Trachomatis IgA ELISA Test Principle:
Purified Chlamydia Trachomatis antigen is coated on the surface of microwells.
Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgA specific
antibody, if present, binds to the antigen. All unbound materials are washed away.
After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess
enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The
enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the
color generated is proportional to the amount of IgA specific antibody in the sample.
The results are read by a microwell reader compared in a parallel manner with
calibrator and controls.



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