HAV IgM ELISA kit description:

"Export Use Only"
The HAV IgM ELISA test is an enzyme-linked immunosorbent assay (ELISA) which is used for the qualitative determination of IgM-class antibodies to hepatitis A virus (HAV-IgM) in serum/plasma. The purpose of the HAV IgM ELISA Test is for diagnosing and monitoring of patients suspected of hepatitis A virus.




Materials Provided with HAV IgM ELISA Kit:
1. Microwell strips: anti-IgM antibodies coated wells
2. Negative Control: Protein-stabilized buffer
3. Positive Control: Purified anti-HAV IgM antibodies diluted
4. HRP Conjugate: HRP-HAV antigen
5. Stock Wash Buffer 20x
6. Chromogen Solution A: Urea peroxide solution.
7. Chromogen Solution B: TMB
8. Stop Solution
9. Plastic Sealable Bag
10. Cardboard Plate Cover

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




HAV IgM ELISA Test Background Information:
Infections usually onset in early childhood, HAV can adversely affect the liver, but acute complications or chronic illness do not necessarily occur because Hepatitis A is a self-limited disease. Causes of the infection are connected to unsanitary conditions and poor hygiene within densely populated areas. Thus, the fecal-oral route of the disease is easily transmitted in this type of setting. One single infected person could cause an entire outbreak. Hepatitis A is a non-enveloped positive strand RNA virus with a linear single strand genome, encoded for only one known serotype. Within HAV are four major, structural polypeptides, concentrated exclusively in the cytoplasm of human hepatocites. An HAV infection causes a strong immunological response, and within a few days after the onset of symptoms, elevated levels of IgM, and subsequently IgG, are identified. The identification of anti-HAV IgM serves as a critical serological marker in detection and observation of how the disease has manifested in a clinical setting. After approximately three weeks of exposure, increasing levels of anti-HAV IgM are detectable, with the highest titter occurring after four to six weeks.





HAV IgM ELISA Test Principle:
The principle method of the HAV IgM ELISA is a solid phase two-step incubation, antibody capture ELISA assay. In this HAV IgM ELISA kit, polystyrene microwell strips are pre-coated with antibodies to human immunoglobulin M proteins (anti-u chain). During the first incubation, the serum or plasma sample is added. At this point, any IgM antibodies will be captured in the wells. Then HAV antigens conjugated to HRP-Conjugate is added, and any specific HAV IgM captured during the solid phase will be identified at this time. In the course of second incubation, the HRP-conjugated antigens will react only with the HAV IgM antibodies.



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* Not to be sold, shipped or distributed in The United States.