Adenovirus IgM ELISA kit description:

The Diagnostic Automation Adenovirus IgM Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgM antibodies against Adenovirus in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of Diagnostic Automation.





Materials Provided with Adenovirus IgM ELISA Kit:
1. Adenovirus IgM Test Microtiter, wells coated with a Adenovirus Hexon
2. Adenovirus IgM EIA Kit Calibrator A (Negative Control)
3. Adenovirus IgM EIA Kit Calibrator B (Cut-Off Standard)
low concentration of IgG antibodies against Adenovirus
4. Adenovirus IgM EIA Kit Calibrator C (Weak Positive Control)
medium concentration of IgG antibodies against Adenovirus
5. Adenovirus IgM EIA Kit Calibrator D (Positive Control)
high concentration of IgG antibodies against Adenovirus
6. Adenovirus IgM Elisa Kit Enzyme Conjugate, anti-human-IgM-HRP (rabbit)
7. Adenovirus IgM Elisa Kit Substrate, TMB
8. Stop Solution
9. Sample Diluent
10. Adenovirus IgM Elisa Kit Test Washing Buffer
11. Plastic Foils
12. Plastic Bag

Materials & Instrumentations required but not included:
1. Reagent tubes for the serum dilution
2. Bidistilled water
3. Precision pipettes
4. ELISA Kit Microplate Washer
5. ELISA Kit Microplate Reader at 450





Adenovirus IgM Antibody ELISA Kit Background Information
The adenovirus is a ubiquitous pathogen of humans and animals. Adenoviruses are characterized by location inside the cell nucleus, common complement-fixing antigens and marked stability to environmental effects. Adenoviruses are endemic in all populations throughout the year. The infection is spread both through the aerial-droplet route and the routes characteristic for intestinal infections. The incubation period is between five and seven days. Adenoviruses mainly infest respiratory and intestinal mucosa, but also the cornea. They are accumulated in the epithelial cells and regional lymph nodes. Adenoviruses cause the widest variety of illnesses of the known respiratory viruses. The adenovirus infection is the most frequently caused viral disease of the respiratory tract among preschool children. Acute diseases of the upper respiratory tract occur predominantly. An acute adenoviral infection can be detected by virus isolation and/or serology. The serologic tests are particularly important because they document actual infection in the patient and can be applied to large-scale epidemiologic investigations. The CF and ELISA tests measure predominantly the antibodies directed against the group-specific determinants on the hexon component. The recommended tests for measuring type-specific antibodies are hemagglutinin inhibition and serum neutralization. The type-specific antigenic determinants of adenoviruses are located at the fibers on the capsid. Because of the ubiquity of the adenoviruses and numerous cross-reactions between related serotypes, seroconversion involving a fourfold or greater rise in antibody infection is necessary to document infection.




Adenovirus IgM Antibody ELISA Test Principle
Adenovirus antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Adenovirus antigen takes place. After one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color.




Adenovirus IgG ELISA test kit Qualitative EvaluationThe calculated absorptions for the patient sera are compared with the value for the cutoff standard. If the value of the sample is higher, there is a positive result. For a value below the cut-off standard, there is a negative result. It is recommended to define a range of +/- 20 % around the value of the cut-off as a grey zone. In such a case the repetition of the test with the same serum or with a new sample of the same patient, taken after 2-4 weeks, is recommended. Both samples should be measured in parallel in the same run. The positive control must show at least the double absorption compared with the cut-off standard.




Adenovirus IgM Antibody Related ELISA Kits:

Adenovirus IgA ELISA kit

http://www.rapidtest.com/index.php?i=Adenovirus-IgA-ELISA-kit&id=90&cat=16

Adenovirus IgG ELISA kit

http://www.rapidtest.com/index.php?i=Adenovirus-IgG-ELISA-kit&id=91&cat=16



Diagnostic Automation Inc. also provides other Infectious Disease ELISA Kits:

http://www.rapidtest.com/index.php?product=Infectious-Disease-ELISA-kits&cat=16



For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.