Lyme Disease IgG ELISA Test description:

Lyme Disease IgG ELISA test kit is a B. burgdorferi ELISA test system designed to detect IgG class antibodies to B. burgdorferi in human sera.




Material Provided with B burgdorferi IgG ELISA Kit:
1. Plate: wells coated with inactivated B burgdorferi (B31 strain) antigen
2. HRP-Conjugate goat anti-human IgG
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Absorbent Solution: goat anti-human
7. Sample Diluent
8. TMB
9. Stop Solution
10. Wash Buffer Concentrate 10X
11. Package Insert

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




B burgdorferi IgG ELISA Kit Background Information:
Borrelia burgdorferi is a spirochete that causes Lyme disease. The organism is transmitted by ticks of the genus Ixodes. Patients with early infection produce IgM antibodies during the first few weeks after onset of erythema migrans (EM) and produce IgG antibodies more slowly. Although IgM only may be detected during the first month after onset of illness, the majority of patients develop IgG antibodies within one month. Both IgG and IgM antibodies can remain detectable for years. Serological testing methods for antibodies to B. burgdorferi include indirect fluorescent antibody (IFA) staining, immunoblotting, and enzyme immunoassay (EIA). B. burgdorferi is antigenically complex with strains that vary considerably. Early antibody responses often are to flagellum which has cross reactive components. Patients in early stages of infection may not produce detectable levels of antibody. Also, early antibiotic therapy after EM may diminish or abrogate good antibody response. Some patients may never generate detectable antibody levels. Thus, serological tests for antibodies to B. burgdorferi are known to have low sensitivity and specificity and because of such inaccuracy, these tests cannot be relied upon for establishing a diagnosis of Lyme disease.


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B burgdorferi IgG ELISA Test Principle:
Borrelia burgdorferi ELISA test kit is designed to detect IgG class antibodies to B. burgdorferi in human sera. Wells of plastic microwell strips are sensitized by passive absorption with B. burgdorferi antigen. The test procedure involves three incubation steps. For additional details please refer to the instructions for use.



This Lyme Disease elisa kit is designed to detect IgG class antibodies to B. burgdorferi in human sera. Diagnostic Automation provides two other Lyme Disease ELISA Test Kits:
1. Lyme Disease IgM ELISA Test Kit, designed to detect IgG antibodies to B. burgdorferi in human sera.
2. Combination Lyme Disease IgG/IgM ELISA Test Kit, designed to detect IgM and IgG class antibodies to B. burgdorferi in human sera.

For more information about these Lyme Disease ELISA Test Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .