Syphilis (TPA) IgG ELISA Test description:

Syphilis (TPA) IgG ELISA kit is an enzyme-linked immunosorbent assay for the qualitative detection of IgG antibodies to T. palladium in serum specimens




Material Provided with Syphilis (TPA) IgG ELISA Kit:
1. Microwell Strips: wells coated T. pallidum antigens
2. High titre reactive control (HTR): human serum
3. Low Titre reactive control (LTR): human serum
4. Non-reactive control (N): human serum
5. Conjugate-HRP
6. Wash Buffer 20X
7. Sample Diluent
8. Substrate Solution
9. Plate Sealers

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




Syphilis (TPA) IgG ELISA Kit Background Information:
Syphilis is a disease, usually sexually transmitted, and caused by infection with the spirochete Treponema pallidum (T.pallidum). Clinical diagnostic serum specimens which are reactive in reagin tests are typically confirmed using treponemal tests such as the Microhaemagglutination-T. pallidum (MHA-TP) or the Fluorescent Treponemal Antibody-Absorption (FTA-ABS) test. In contrast to the non-treponemal tests, treponemal test reactivity will persist following treatment in approximately 85% of the cases often for the life of the patient. Any sera giving reactive or equivocal results on initial treponemal based assays must be supplemented with a quantitative non-treponemal test (such as RPR or VDRL) to distinguish from active disease and assist in ruling out false positives. Syphilis-IgG is a treponemal test for T. pallidum IgG class antibodies. The enzyme immunoassay format allows use of a micorplate reader which eliminates subjective interpretation of results and the procedures for the syphilis test kit can be automated for high-volume testing.


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Syphilis (TPA) IgG ELISA Test Principle:
Microtitration wells coated with T. pallidum antigens are exposed to test specimens. After an incubation period, unbound components in the test sample are washed away. Specifically-bound IgG reacts with a conjugated horseradish peroxidase monoclonal antibody during a second incubation period. Following a second wash cycle, specifically bound enzyme conjugate is detected by reaction with TMB. After stopping the enzymatic reaction, the assay is measured. For additional details please refer to the instructions for use.





Diagnostic Automation Inc. also provides other Infectious Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .