Toxo IgM ELISA kit
| Name |
Toxo IgM ELISA Test |
|---|---|
| Full name |
Human Toxoplasma gondii IgM (Toxo IgM) ELISA Test Kit |
| Category Name | Infectious Disease ELISA kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | Sandwich Complex |
| Detection Range | Qualitative elisa assay- Positive, Negative and Cut-off |
| Sample | 10ul |
| Specificity | 100% |
| Sensitivity | 100% |
| Total Time | ~75min |
| Shelf Life | 12 Months from the manufacturing date |
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Toxo IgM ELISA kit description:
The Diagnostic Automation Toxoplasma IgM is intended for use in the detection of IgM to Toxoplasma gondii.
Materials Provided with Toxo IgM ELISA Kit:
1. Microassay plate:wells coated with Purified Toxoplasma gondii antigen
2. Calibrator: Human serum or defibrinated plasma
3. Positive Control: Human serum or defibrinated plasma
4. Negative Control: Human serum or defibrinated plasma
5. HRP Conjugate: Ready to use, Goat anti-human IgM
6. Serum Diluent Plus, Ready to use
7. Wash Buffer Type I, 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: Ready to use,1N H2SO4 solution
Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450 single & 600-650nm dual wavelength
Toxo IgM ELISA Kit Background Information:
Toxoplasmosis is caused by the intracellular parasite Toxoplasma gondii and may be contracted by consuming contaminated meat or by contact with cat feces containing oocysts. In adolescence and adulthood, most infections are subclinical. However, if a pregnant woman contracts toxoplasmosis, it may be passed through the placenta to the fetus, resulting in congenital toxoplasmosis, which is a cause of mortality and malformation. Asymptomatic infants may develop anomalies later in life.
Toxo IgM ELISA Test Principle:
Purified Toxoplasma gondii antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Toxoplasma gondii IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibodyantigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results of the toxo IgM test are read by a microwell reader compared in a parallel manner with calibrator and controls.
For more information about the Toxoplasma Gondii Test, ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.
Product Note:
When using DAI Toxoplasma IgM ELISA, store serum between 2 and 8 C if testing will take place within two days. If specimens are to be kept for longer periods, store at -20 C or colder. Do not use a frost-free freezer because it may allow the specimens to go through freeze-thaw cycles and degrade antibody. Samples that are improperly stored or are subjected to multiple freeze-thaw cycles may yield erroneous results.