Varicella-Zoster IgM ELISA Test description:

Varicella-Zoster Virus (VZV) IgM ELISA test kit is an Enzyme-linked Immunosorbent Assay intended for the detection and quantitative determination of IgM antibody to VZV in human sera as an aid in the diagnosis of primary infection or reactivation.




Materials provided with Varicella-Zoster IgM ELISA kit:
1. Plate: wells coated with inactivated Varicella-zoster virus antigen
2. Serum Diluent Type I
3. Calibrator: Human serum or defibrinated plasma
4. Positive Control: Human serum or defibrinated plasma
5. Negative Control: Human serum or defibrinated plasma
6. HRP-Conjugate goat anti-human IgM
7. Chromogen/Substrate Solution Type I
8.Wash Buffer 20X
9. Stop Solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm





Varicella-Zoster IgM elisa kit Background Information:
Varicella, more commonly known as chickenpox and herpes zoster are known clinical manifestations of infection with varicella-zoster virus (VZV). There are various methods of serodiagnositic tests for the detection of VZV antibodies in a patient's serum including: indirect immunofluorescence, neutralization, complement fixation and fluorescent antibody to membrane antigen (FAMA). FAMA is generally considered the most sensitive and specific of the methods, yet requires the use of cell culture which is cumbersome to perform. Clinical and correlation studies indicate that the ELISA methodology may be as sensitive and perhaps more specific than the FAMA assay. The sensitivity, specificity, and reproducibility of enzyme-linked immunoassays is comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination, and radioimmunoassays.




Varicella-Zoster IgM elisa Test Principle:
The Varicella-Zoster virus test kit is an Enzyme-Linked Immunosorbent Assay. Linked Immunosorbent Assay. Purified inactivated preparation of Varicella-zoster virus antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Varicella-Zoster IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. For additional details please refer to the instructions for use.



Diagnostic Automation Varicella-Zoster Virus IgM ELISA kit provides all the necessary reagents for the rapid quantitative determination of VZV IgM antibody in human sera.
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