Dengue IgG/IgM ELISA kit description:

"Export Use Only"
Diagnostic Automation Inc.Dengue IgG/IgM ELISA Test is an enzyme linked immunosorbent assay for the qualitative identification of antibodies to dengue in human serum.




Materials Provided with Dengue IgG/IgM ELISA Kit:
1. Dengue IgG/IgM Elisa Microplate: 96 wells coated with Antigens
2. Enzyme Conjugate Anti- human IgG + IgM
3. Dengue IgG/IgM EIA Test Positive Control (+ human serum)
4. Dengue IgG/IgM EIA Test Negative Control (- human serum)
5. Chromogen Solution
6. Wash Concentrate 20X
7. Dilution Buffer
8. Stop Solution

Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Dengue IgG/IgM ELISA Kit Background Information:
Dengue fever, caused by any of the four serotypes of dengue virus is endemic in Southeast Asia and South and Central America. Repeat infection with a second type of dengue virus is thought to cause dengue hemorrhagic fever in about 10 percent of infected people. Dengue antibodies do not confer immunity beyond 3-6 weeks to a second dengue virus type. Infection with dengue virus can result in a wide disease spectrum, from a mild fever to life-threatening dengue hemorrhagic fever and dengue shock syndrome. Symptoms of classical dengue fever, following a 5-8 day incubation period, include rash, severe headache, nausea, vomiting, chills, malaise, macular rash and may include lymphadenopathy. Hemorrhagic dengue fever involves increased blood vessel permeability which can lead to shock and death in about 10% of reported cases. Dengue fever can only be treated by supportive care and is prevented by mosquito control.
In primary infections, circulating IgM antibody to the viral coat proteins is detected 5-6 days after the onset of illness, and gradually decreases within 1-2 months of onset. IgG antibody to dengue virus is detected approximately 14 days after onset in primary infections, and by day 2 in secondary infections. In secondary infections, IgM antibody may reappear but gradually diminishes, while IgG antibody persists, often at high titer. These patterns of dengue antibody development permit serological differentiation of primary and secondary infections. Characteristically, acute patients with primary infections have a higher IgM:IgG ratio than are found in secondary infections. Patients with secondary infections generally have higher IgG levels. Acute or recent infections are identified by a rise in antibody titer as well as high IgM levels.



Dengue IgG/IgM ELISA Test Principle:
Dengue IgG/IgM ELISA kit is a semi-quantitative enzyme immunoassay for the detection of antibodies to dengue in samples of human serum. The microwells of the Dengue IgG/IgM ELISA kit are coated with purified dengue virus antigen from Vero cell cultured type 1-4 dengue. For additional details please refer to the instructions for use.



Dengue IgG/IgM ELISA Kit provides an excellent methodology for specifically detecting antibodies to dengue virus in human serum. The Dengue ELISA Kits are available in several formats: Dengue IgG ELISA Kit, Dengue IgM ELISA Kit, Dengue IgG/IgM ELISA Kit, and Dengue NS1 ELISA Kit for detection of NS1 antigens in human serum. For more information on any of the Dengue ELISA Kits or Dengue NS1 ELISA Kit, please contact our Customer Service Department at 818-591-3030.


* Not to be sold, shipped or distributed in The United States.