Leptospira IgG IgM ELISA kit description:

The Leptospira IgG IgM ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative detection of antibodies to Leptospira biflexa (serovar patoc 1) in serum and plasma.




Material Provided with Leptospira IgG IgM ELISA kit:
1. Test Strips Microwells: 96 test wells containing Leptospira antigen
2. Conjugate IgG/IgM: anti-human IgG/IgM antibody conjugated to peroxidase
3. Positive Control: diluted surrogate positive control
4. Negative Control: diluted negative human serum
5. TMB Substrate Solution: TMB
6. Wash Concentrate 20X
7. Dilution Buffer
8. Stop Solution: 1 M phosphoric acid

Materials Required, Not Provided:
1.Pipettes
2.Squeeze bottle for washing strips (narrow tip is recommended)
3.Reagent grade (DI) water
4.Graduated cylinder
5.Sample Dilution Tubes
6.Absorbent paper
7.ELISA plate reader with a 450 nm and a 620-650 nm filter (optional if results are read visually




Leptospira IgG IgM ELISA kit Background Information:
Leptospira infections in humans are transmitted from infected animals, or by exposure to areas inhabited by contaminated animals. Another source of infection is derived from swimming or bathing in contaminated water, as the infection enters through mucosal eye surfaces or skin contusions. Infected animals may include rats and mice, but also larger mammals (such as dogs, sheep, cows, deer, rabbits, etc.) are primary sources of infection. The leptospira bacteria are shed through the urine of infected animals. Incubation period of the infection in humans is normally 10 to 12 days, but can also range from 3 to 30 days. Antibody detection occurs around the 6th to 10th day of disease, and within 3 to 4 weeks, they reach their peak level. Even though antibody levels slowly abate, they may persist and show up in diagnosis years afterward. The extent of symptoms can range anywhere from mild inflammation of the mucous membrane to jaundice, where severe kidney and liver infections are detected. The Leptospira ELISA kit is the ideal test for acute leptospirosis infections.




Leptospira IgG IgM ELISA Test Principle:
The principle of the Leptospira ELISA test is a three-incubation process with the first incubation involving the coating of the wells with purified Leptospira Patoc1 antigen. Next, the wells must be washed to remove test sample and then Enzyme Conjugate is added. In the presence of this enzyme complex and peroxide, a substrate is added, and as a result of this combination, the substrate color will turn blue. The reaction is ended with the stop solution, and what appears is a yellow color instead of the blue.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Leptospira IgG IgM ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.