Schistosoma IgG ELISA Kit description:

The Schistosoma IgG ELISA test is a qualitative enzyme immunoassay for the detection of antibodies to Schistosoma, in samples of human serum or plasma.




Materials Provided with Schistosoma ELISA Kit:
1. Schistosoma EIA Plate:containing Schistosoma recombinant antigens
2. Enzyme Conjugate: Protein A conjugated to peroxidase.
3. Schistosoma Elisa Kit Positive Control: diluted positive rabbit sera
4. Negative Control: diluted human sera
5. Chromogen: TMB
6. Schistosoma EIA Wash Concentrate 20X
7. Dilution Buffer: buffered protein solution
8. Stop Solution: 1 M phosphoric acid

Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Schistosoma ELISA Assay Kit Background Information:
Schistosomiasis (also known as bilharzia, bilharziosis or snail fever) is a collective name of parasitic diseases caused by several species of trematodes belonging to the genus Schistosoma. Snails serve as the intermediary agent between mammalian hosts. Individuals within developing countries who cannot afford proper water and sanitation facilities are often exposed to contaminated water containing the infected snails. Although it has a low mortality rate, schistosomiasis often is a chronic illness that can damage internal organs and, in children, impair growth and cognitive development. The urinary form of schistosomiasis is associated with increased risks for bladder cancer in adults. Schistosomiasis is the second most socioeconomically devastating parasitic disease after malaria.



Schistosoma ELISA Test Principle:
The microwells are coated with Schistosoma recombinant antigen. During the first incubation with the diluted patients sera, antibodies which are reactive with the antigen will bind to the coated wells. After washing, the Enzyme Conjugate is added. The Enzyme Conjugate will then bind to these antibodies. After another series of washes, in the presence of this enzyme complex and peroxide, a substrate is added, and as a result of this combination, the substrate color will turn blue. The reaction is ended with the stop solution, and what appears is a yellow color instead of the blue. For additional details please refer to the instructions for use.


Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Schistosoma IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.