Cysticercosis IgG ELISA kit description:

Cysticercosis ELISA kit is an ELISA test for the qualitative identification of serum antibodies (IgG) to Taenia solum antigen.




Material Provided with Cysticercosis ELISA kit:
1.Test Strips Microwells: 96 test wells containing T. solium antigens
2. Enzyme Conjugate: Protein A conjugated to peroxidase
3. Positive Control: diluted positive rabbit serum
4. Negative Control: diluted negative human serum
5. TMB Substrate Solution: TMB
6. Wash Concentrate 20X
7. Dilution Buffer: buffered protein solution
8. Stop Solution: 0.73 M phosphoric acid

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm and a 620 - 650 nm filter





Cysticercosis ELISA kit Background Information:
The causative agents of Taenia solum infection are generally associated with contaminated food, infected workers, gastric reflux in tapeworm carriers, or contaminated water. These contamination routes are generally created by ingestion of T. solum eggs. Cysticercosis is known as an infection of the larval form (cysticeri) of Taenia in organs or tissues. Areas most affected by the infection revolve around the central nervous system. In addition, Cysticercosis can cause seizures and cranial pressure in the brain. This Cysticercosis ELISA test should not be the sole method of diagnosis. Because of possible cross reactions with Echinococcosis, other testing procedures should be done so that the Echinococcus infection can be ruled out. Even though cyst vesicular antigen aids in sensitivity and specificity, other non-serological tests are recommended to verify accurate results of the T. solum infection.





Cysticercosis ELISA Test Principle
Before the first incubation, the microwells are coated with T. solum cyst antigen. The patient sera is added, and if there are any antibodies present, they will bind to the wells during the first incubation. Next, the wells must be washed of any test sample, and added to the wells at this point is the Enzyme Conjugate. During the second incubation, the Enzyme Conjugate will bind to any antibodies present. Then a chromogen TMB is added and color develops.



Notable features of the Parasitology ELISA Kits
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Cysticercosis ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.