Toxocara IgG ELISA kit description:

The Toxocara IgG ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative detection of IgG antibodies to Toxocara in human serum.




Materials Provided with Toxocara ELISA Kit:
1. Toxocara EIA Plate: containing Toxocara antigens
2. Enzyme Conjugate: Protein A conjugated to peroxidase.
3. Positive Control: of diluted positive rabbit serum
5. Negative Control: diluted negative human serum
4. TMB Substrate Solution
6. Toxocara EIA Wash Concentrate 20X
7. Dilution Buffer: buffered protein solution
8. Stop Solution: 11ml of 1 M phosphoric acid

Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Toxocara ELISA Assay Kit Background Information:
Infected humans cannot pass the Toxocara infection to other humans. Causes of the toxocariasis infection stem from swallowing dirt containing Toxocara eggs from animal feces. The infection is caused by roundworm larvae of two species of Toxocara - (T. canis) from dogs and (T.cati) from cats. Toxocara is not contagious from one human to another, and because the eggs become hatched after 2 to 5 weeks, humans do not acquire this infection from fresh feces. Toxocara larva migrans has been labeled the second most common parasitic worm in many countries throughout the world. Toxocara infections migrate to different parts of the body and consequently can develop into varied symptoms. One common type is OLM (ocular larval migrans), where the larva migrates to the eye. Symptoms range from asymptomatic to serious eye infections. Another type of manifestation is VLM (visceral larval migrans), where the body's organs, or central nervous system, are infected. Patients with VLM may experience serious fatal conditions, develop a blood disorder, or show no signs of symptoms at all.




Toxocara ELISA Test Principle:
The principle of the Toxocara ELISA test is a three-incubation process with the first incubation involving the coating of the wells with excretory/secretory antigen from the Toxocara larvae. Then incubation with the diluted patients sera, antibodies which are reactive with the antigen will bind to the coated wells. After washing, the Enzyme Conjugate is added. The Enzyme Conjugate will then bind to these antibodies. After another series of washes, in the presence of this enzyme complex and peroxide, a substrate is added, and as a result of this combination, the substrate color will turn blue. The reaction is ended with the stop solution, and what appears is a yellow color instead of the blue. For additional details please refer to the instructions for use.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Toxocara IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.