Trichinella IgG ELISA Kit description:

The Trichinella ELISA Test is an enzyme-linked immunosorbent assay for the qualitative detection of IgG antibodies to Trichinella in serum.




Materials Provided with Trichinell IgG ELISA Kit:
1. Trichinell EIA Plate: containing Trichinella antigens
2. Enzyme Conjugate: Protein A conjugated to peroxidase.
3. Positive Control: of diluted positive rabbit serum
5. Negative Control: diluted negative human serum
4. TMB Substrate Solution
6. Trichinell EIA Wash Concentrate 20X
7. Dilution Buffer: buffered protein solution
8. Stop Solution: 1 M phosphoric acid

Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter




Trichinell ELISA Assay Kit Background Information:
One of the most widespread parasites in the world is the nematode Trichinella spiralis, which causes Trichinosis. This nematode parasite invades animals, and the worms travel through the intestine, blood, and lymphatic system, eventually being carried to muscles. Humans are infected when they ingest uncooked meats of these animals, but the primary host of Trichinosis is that belonging to the swine. Serological testing is one of the most common methods used for early identification of trichinosis. ELISA tests have been shown to be an important procedure in detecting trichinosis infections. Other methods include Latex agglutination, Indirect hemagglutination, and Bentonite flocculation. Several important studies have been carried out to test the performance of the various methods. Besides the ELISA test, the Bentonite Flocculation (BFT) has been frequently used; however, its nonspecific reactions, difficulty in performing the test, and lack of sensitivity (3-4 weeks to detect antibodies), have been problematic when screening for Trichinella. What has been found to have a high specificity in many studies is an excretory-secretory (ES) antigen that comes from larvae of infected swine.




Trichinell ELISA Test Principle:
The principle of the Toxocara ELISA test is a three-incubation process with the first incubation involving the coating of the wells with purified Trichinella antigen. Then incubation with the diluted patients sera, antibodies which are reactive with the antigen will bind to the coated wells. After washing, the Enzyme Conjugate is added. The Enzyme Conjugate will then bind to these antibodies. After another series of washes, in the presence of this enzyme complex and peroxide, a substrate is added, and as a result of this combination, the substrate color will turn blue. The reaction is ended with the stop solution, and what appears is a yellow color instead of the blue. For additional details please refer to the instructions for use.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Trichinell IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.