E.coli Verotoxin (Fecal) ELISA kit description:

The Diagnostic Automation Inc. E. coli Verotoxin ELISA kit is an in vitro procedure for the qualitative determination of verotoxin in feces. It is a double antibody (sandwich) ELISA using anti-verotoxin antibodies to capture the antigen from the stool supernatant. A second anti-verotoxin monoclonal antibody cocktail is then added, which binds to the complex. This reaction is visualized by the addition of anti-mouse antibodies conjugated to peroxidase. The resulting blue color following the addition of the chromogen indicates the presence of verotoxin being bound by the anti-verotoxin antibodies.



Materials Provided with E. coli Verotoxin ELISA kit:
1. Test Strips, 96 Microwells coated with rabbit anti-verotoxin (VT1 &VT2)
2. Verotoxin EIA monoclonal anti-verotoxin antibodies, 11ml
3. Verotoxin EIA anti-mouse antibody HRP-conjugate, 11ml
4. Verotoxin ELISA Positive Control 1 vial, 2 ml
5. Verotoxin ELISA Negative Control 1 vial, 2 ml
6. Chromogen, 1 bottle, 11 ml of TMB and peroxide
7. Verotoxin ELISA Wash solution (20X), 25 ml
8. Stop Solution 1 bottle, 11 ml

Materials Required But Not Provided:
1. Precision pipettes
2. Reagent grade DI water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 & 620-650 nm filters



E. coli Verotoxin ELISA kit Background information
The verotoxin (VT), or shiga-like toxin family is a group of closely related toxins produced by certain
strains of Escherichia coli.4 These strains are a significant cause of human hemorrhagic colitic (HC) and hemolytic uremic syndrome (HUS). In addition, they are both water borne and food borne and may also be transmitted from person-to-person by the oral-fecal route. In adults, illness caused by verotoxin
may last several days, while in children and the elderly, the illness can be fatal. Infections occur as sporadic illnesses or outbreaks, and are distributed throughout North and South America, Europe, and Asia. The peak incidence of infection occurs during summer months.4 In the past, lengthy and cumbersome diagnostic procedures have been utilized for the detection of verotoxin. Currently, simple to perform enzyme-linked immunosorbent assays (ELISA) are being used as an alternative to these longer tedious methods. These antigen-detection assays provide a cost-effective tool for the management of diarrheal disease with an accurate and rapid test.



E. coli Verotoxin ELISA Test Principle
During the first incubation, verotoxin present in the stool supernatant is captured by antibodies attached to the wells. The second incubation adds an additional anti-verotoxin antibody cocktail that sandwiches the antigen. The next incubation attaches horseradish peroxidase to the sandwich. After
washings to remove unbound enzyme, a chromogen is added which develops a blue color in the presence of the enzyme complex and peroxide. The stop solution ends the reaction and turns the blue color to yellow.



Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the E. coli Verotoxin ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.


Related ELISA Kit:

E.Coli O157 (Fecal) ELISA kit (E.coli ELISA kit)

Diagnostic Automation Inc. offers E.Coli O157 (Fecal) ELISA kit. E. coli O157 has been implicated as the causative organism of hemolytic uremic syndrome (HUS) and hemorrhagic colitis (HC).

http://www.rapidtest.com/index.php?i=E.Coli-O157-(Fecal)-ELISA-kit&id=186&cat=17

Diagnostic Automation Inc. also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.