West Nile IgG ELISA kit description:

The West Nile IgG Test is an ELISA assay system for the detection of antibodies in human serum to West Nile Virus (WNV) derived recombinant antigen (WNRA). This test is to aid in the diagnosis of human exposure to the West Nile Virus.




Materials Provided with West Nile IgG ELISA Kit:
1. West Nile EIA Plate: coated with monoclonal antibody bound to recombinant WN antigen
2. Sample Dilution Buffer for IgG
3. WN IgG Positive Control (IgG P)
4. WN IgG Negative Control (IgG N)
5. Enzyme Conjugate-HRP for WN IgG
6. 10X Wash Buffer
7. Wash Solution
8. Liquid TMB Substrate
9. Stop Solution


Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm filter




West Nile ELISA Assay Kit Background Information:
Exposure to West Nile Virus causes a disease with a number of symptoms including encephalitis. West Nile Virus is becoming widespread and has been detected in over half of the 50 states. This test has been developed and refined using reagents produced by CDC. The West Nile Detect assay employs a recombinant antigen called WNRA, which can be used as a rapid serological marker for WNV infection. The WNRA protein is a recombinant antigen, which consists of a stretch of peptides from two WNV antigens.




West Nile ELISA Test Principle:
The West Nile Detect IgG ELISA consists of one enzymatically amplified two-step
sandwich-type immunoassay. Wells are incubated with standards, controls or
unknown serum samples. After washing, the wells are treated with an antibody specific for human IgG and labeled with the enzyme HRP. After a second incubation and washing step, the wells are incubated with the TMB substrate. An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. For additional details please refer to the instructions for use.





Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the West Nile IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.