Crypto/Giardia Ag Combo ELISA kit description:

Crypto/Giardia ELISA kit test is an in vitro immunoassay for the qualitative determination of Giardia and Cryptosporidium antigen in feces.




Material Provided with Crypto/Giardia Ag combo Fecal ELISA kit:
1. Test Strips Microwells: 96 test wells anti-Giardia and anti-Cryptosporidium antibodies
2. Enzyme Conjugate: anti-Giardia and anti-Cryptosporidium antibodies
3. Giardia Positive Control: diluted Giardia positive formalinized stool supernatant
4. Cryptosporidium Positive Control: Cryptosporidium positive formalinized stool supernatant
5. Negative Control: a G/C negative formalinized stool supernatant
6. Chromogen: TMB
7. Wash Concentrate 20X
8. Dilution Buffer: a buffered protein solution with Thimerosal
9. Stop Solution: 5% phosphoric acid

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm and a 620 - 650 nm filter




Crypto/Giardia ELISA kit Background Information:
Giardia lamblia is the protozoan parasite responsible for the disease giardiasis. Symptoms of acute giardiasis include diarrhea, nausea, weight loss, malabsorption, abdominal cramps, flatulence and anemia. The disease may manifest itself as an acute, chronic or as an asymptomatic infection. Giardiasis is the most prevalent parasitic disease in the United States and is responsible for an estimated 100 million mild infections and 1 million severe infections each year. The mode of transmission of Giardia is through fecal-oral ingestion of cysts. Epidemics of giardiasis have been documented in day care centers and by drinking contaminated water. Day care centers may be directly or indirectly responsible for 45% of diagnosed Giardia infections in the United States. Another important source of Giardia infection is among homosexual men. Prevalence rates of 5 to 19% for this population have been reported. Cryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men.




Crypto/Giardia ELISA kit Test Principle:
During the first incubation, Giardia and/or Cryptosporidium antigens present in stool specimens are captured by antibodies attached to the microwells. The wells are incubated and washed before anti-Giardia and anti-Cryptosporidium antibodies conjugated to peroxidase are added. The enzyme conjugate will sandwich any antigen bound to the wells. After washings to remove any unbound enzymes, a Chromogen is added, which develops a blue color in the presence of the enzyme complex.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Crypto/Giardia ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.