Dengue NS1 ELISA kit - Antigen description:

"Export Use Only"
Diagnostic Automation offers a Dengue NS1 Antigen ELISA kit which is an Enzyme Linked Immunosorbent Assay for the detection of the Dengue virus. This Dengue NS1 ELISA kit will aid in the early diagnosis of Dengue virus in human serum even prior to the presence of lgM or lgG antibodies. The Dengue NS1 ELISA kit is a highly sensitive, rapid and reliable assay.




Material Provided with Chagas ELISA kit:
1. Test Strips Microwells: 96 test wells containing Dengue NS1
2. Enzyme Conjugate: 100x Conjugate for Dengue NS1
3. Dengue NS1 Positive Control
4. Dengue NS1 Negative Control
5. Dengue NS1 Cut-Off Control: contains the secondary antibody
6. Sample Diluent for Dengue NS1
7. Conjugate Diluent for Dengue NS1
8. Wash Buffer 10X
9. TMB Substrate Solution: TMB
10. Stop Solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm and a 620 - 650 nm filter





Dengue NS1 Antigen ELISA kit Background Information
Dengue is an acute viral disease which is transmitted by Aedes aegypti mosquitoes. Dengue is characterized clinically by biphasic fever, rash and hematopoietic depression, and by constitutional symptoms such as malaise, arthralgia, myalgia, and headache. Infrequently, more severe disease is seen, manifested by hemorrhage fever which may progress to lethal shock. Dengue NS1 (non-structural) protein is a hexametric secreted protein. It is believed to play a role in viral RNA replication. Dungue NS1 is strongly immunogenic eliciting antibodies with complement fixing activity. The use of the Dengue NS1 ELISA kit can detect NS1 antigen in circulating blood during acute Dengue infection. In addition, this Dengue NS1 ELISA kit can detect NS1 antigen in serum samples almost immediately following infection.





Dengue NS1 Antigen ELISA Test Principle
This Dengue NS1 ELISA kit uses one enzymatically amplified, two-step sandwich-type immunoassay to detect low levels of NS1 in serum. In this Dengue NS1 ELISA kit, controls and unknown serum samples are diluted in sample dilution buffer, containing secondary antibody, and incubated in microtitration wells. These wells on the Dengue NS1 ELISA kit have been coated with a highly effective NS1 antibody and then blocked. NS1 antigens present in the samples on this Dengue NS1 ELISA kit are then sandwiched between the capture and secondary antibodies. The presence of NS1 antigen on this Dengue NS1 ELISA kit is confirmed by the colorimetric response obtained using an enzyme-conjugate-HRP and liquid TMB substrate. Product inserts for most Parasitology ELISA kits follow a similar method. See the Dengue NS1 ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.




The Dengue ELISA Kits are available in two formats: Dengue ELISA Kit for detection of lgG, lgM and lgG/lM antibodies, and Dengue NS1 ELISA Kit for detection of NS1 antigens in human serum. For more information on either the Dengue ELISA Kit or Dengue NS1 ELISA Kit, please contact Customer Service.




Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

* Not to be sold, shipped or distributed in The United States.