Ascaris IgG ELISA kit description:

The Diagnostic Automation Ascaris ELISA test is a qualitative enzyme immunoassay for the detection of antibodies to Ascaris, in samples of human serum or plasma.





Material Provided with Ascaris IgG ELISA Kit:
1. Plate: 96 Microwells containing Ascaris antigens
2. Enzyme Conjugate: Protein A conjugated to peroxidase
3. Positive Control: diluted rabbit sera
4. Negative Control: diluted human sera.
5. Chromogen:TMB
6. Wash Concentrate 20X: buffer and surfactant
7. Dilution Buffer: buffered protein solution
8. Stop Solution: 1 M phosphoric acid

Materials Required, not Provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate washer
5. EIA kit Microplate Reader with 450nm or dual wavelength 620-650 nm filter






Ascaris IgG ELISA Kit Background Information:
Ascaris lumbricoides has probably been infecting humans for thousands of years. It is the most common nematode parasite infecting humans and over 1 billion people are believed to be infected. Children who live in moist, warm climates are the most at risk to become infected. Ingesting embryonated eggs from contaminated soil is the primary means of infection. The eggs will hatch in either the stomach or small intestine where the larvae penetrate through the intestine wall. Larvae are carried to the heart and then to the lungs, where they stay for approximately 10 days. Larvae will then go into the alveoli and migrate via the bronchi to the trachea and pharynx.The larvae are coughed up, swallowed, and returned to the intestine where they mature and mate, eventually producing eggs. This process occurs over 8-12 weeks. Eggs will get passed into the environment via feces. Fertilized eggs will become infective within 2 weeks if they are kept in warm, moist soil. The primary means of preventing the spread of Ascaris infection is through the use of appropriate sanitation facilities and practices, such as frequent washing of the hands.





Ascaris IgG ELISA Test Principle:
The microwells are coated with Ascaris antigen. During the first incubation with the diluted patients sera, any antibodies which are reactive with the antigen will bind to the coated wells. After washing to remove the rest of the sample, the Enzyme Conjugate is added. If antibodies have been bound to the wells, the Enzyme Conjugate will then bind to these antibodies. After another series of washes, a chromogen (TMB) is added. If the Enzyme Conjugate is present, the peroxidase will catalyze a reaction that consumes the peroxide and turns the chromogen from clear to blue. Addition of the Stop Solution ends the reaction and turns the blue color to a bright yellow color. The reaction may then be read visually or with an ELISA reader.For additional details please refer to the instructions for use.





Diagnostic Automation Inc. also provides other Parasitology ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .